FastQCFastQC Report
Tue 10 May 2022
EGAF00003670654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761644
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAACTGTACGCTGTACCTGCATCAGCATTAGTAATCAACCTGTTA21760.28569778006522734No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9460.12420500916438652No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9300.12210429019331867No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8830.11593342821580685No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8680.11396400418043076No Hit
GTATGTAGGTAGCCACTGAAAGAATTTGGGCCCCTTGGGAGGATGGCAGT8560.11238846495212987No Hit
GTACCTGCATCAGCATTAGTAATCAACCTGTTAATCCAAGGTCTTTAGAA7870.10332911438939976No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7810.10254134477524933No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7660.10057192073987323No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT7620.10004674099710627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)