FastQCFastQC Report
Tue 10 May 2022
EGAF00003670923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10686
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC390.36496350364963503No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA350.3275313494291597No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT320.2994572337638031No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT300.2807411566535654No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT300.2807411566535654No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT290.27138311809844656No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT270.25266704098820886No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA260.24330900243309003No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA260.24330900243309003No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC240.22459292532285235No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC240.22459292532285235No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT220.20587684821261462No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGC210.19651880965749577No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC200.18716077110237694No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGA200.18716077110237694No Hit
GTGCAAAGAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCT170.1590866554370204No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT170.1590866554370204No Hit
TTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGA160.14972861688190156No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA150.1403705783267827No Hit
GTGCTAGATGGCGCCTGGAAGGGGGGTCACCCAAGTCTCCCTGCTGTCAT150.1403705783267827No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC150.1403705783267827No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT140.13101253977166386No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC140.13101253977166386No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA140.13101253977166386No Hit
GTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGTGG140.13101253977166386No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT130.12165450121654502No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT130.12165450121654502No Hit
CTCTATTTGTACTTGGGAGCAGGGCTGTCCCGACATCAGGCGCAGCAGCT130.12165450121654502No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG130.12165450121654502No Hit
GTACATGGGACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGA130.12165450121654502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.12165450121654502No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT120.11229646266142618No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC120.11229646266142618No Hit
GTGTTGTATTTTTAAAAAAATCCAGGTTGTGCAGGTTGTTCTATTTACAT120.11229646266142618No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC120.11229646266142618No Hit
TGTCTGACGTCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCC120.11229646266142618No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC120.11229646266142618No Hit
CAGCTGCACTTGTCTGACGTCCCTTTGCAGATGCAGCCCTGGGCACACTT120.11229646266142618No Hit
GTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCTTCTCCTTGCC110.10293842410630731No Hit
CCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCA110.10293842410630731No Hit
CTCCTGCACCTGCGCCAGCTCCTGCAAGTGCAAAGAGTGCAAATGCACCT110.10293842410630731No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)