FastQCFastQC Report
Tue 10 May 2022
EGAF00003671066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323243
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC25860.8000173244277524No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT18560.574181034082718No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14210.43960735421958097No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12750.394440096150574No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT11670.3610286997707607No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA11170.3455604607060323No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10610.3282360329535365No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC10310.3189550895146995No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT10060.3112209699823353No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9930.30719922782550585No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9880.30565240391903303No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA9730.30101193219961453No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT8550.2645068880068555No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA8110.25089483762989456No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT7860.24316071809753034No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT7800.24130452940976294No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT7760.24006707028458468No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT7760.24006707028458468No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG7660.23697342247163897No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT7610.23542659856516612No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC7540.23326104509610418No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA7300.22583629034503452No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG7230.22367073687597255No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT7180.2221239129694997No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7100.21964899471914318No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7030.21748344125008123No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG6970.21562725256231383No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6520.20170583740405823No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG6490.20077774306017454No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT6450.19954028393499626No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG6170.19087807005874838No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG5670.17540983099401997No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC5450.1686038058055395No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT5410.1673663466803612No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA5370.16612888755518296No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG5310.16427269886741552No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC5140.15901349758540786No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC5080.15715730889764049No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC5020.15530112020987308No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT5010.15499175542857851No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG5000.15468239064728395No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG4990.15437302586598936No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA4950.15313556674081108No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA4950.15313556674081108No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT4920.1522074723969274No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA4890.15127937805304367No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT4880.1509700132717491No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG4840.14973255414657086No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG4820.14911382458398173No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT4760.1472576358962143No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA4700.1454014472084469No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA4580.14168906983291207No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC4560.14107034027032295No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG4550.14076097548902838No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA4550.14076097548902838No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT4530.14014224592643923No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG4500.13921415158255554No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA4500.13921415158255554No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA4420.136739233332199No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC4410.13642986855090442No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA4400.13612050376960985No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG4390.1358111389883153No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA4370.13519240942572616No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT4350.134573679863137No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA4330.13395495030054788No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA4330.13395495030054788No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG4320.13364558551925332No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT4310.13333622073795875No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC4300.1330268559566642No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG4250.13148003205019135No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA4250.13148003205019135No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC4230.13086130248760222No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA4220.13055193770630763No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG4210.13024257292501307No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC4200.1299332081437185No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC4190.12962384336242394No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT4190.12962384336242394No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG4180.12931447858112935No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA4100.12683956033077282No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT4090.12653019554947825No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA4080.1262208307681837No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA4010.1240552772991217No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA3980.123127182955238No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT3940.12188972383005973No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG3890.1203428999235869No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA3870.11972417036099778No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC3860.1194148055797032No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC3800.11755861689193578No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG3780.11693988732934665No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA3750.11601179298546295No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC3670.11353687473510642No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC3670.11353687473510642No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA3650.11291814517251726No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG3620.11199005082863356No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC3620.11199005082863356No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC3610.111680686047339No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC3530.10920576779698246No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT3520.1088964030156879No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC3500.10827767345309876No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA3490.1079683086718042No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG3470.10734957910921504No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT3470.10734957910921504No Hit
ACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTCAA3460.10704021432792048No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT3460.10704021432792048No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA3440.10642148476533134No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT3440.10642148476533134No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA3430.10611211998403677No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT3420.10580275520274221No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG3420.10580275520274221No Hit
CACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTG3410.10549339042144763No Hit
CTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGG3390.1048746608588585No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG3370.10425593129626937No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC3350.10363720173368024No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT3290.10178101304591283No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC3280.10147164826461826No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA3270.10116228348332368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)