FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003671193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCAGTGCCATGGGCTTCACTGCGGCGGGAATCGCCTCGTCCTCCAT50.18368846436443792No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG50.18368846436443792No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC50.18368846436443792No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG40.1469507714915503No Hit
AGCCTGGGCCACAGAGCAAGACTCCATCTCAAAAAAAAAAAAAGAAAGAA40.1469507714915503No Hit
GTTCTGAGAGTGATCACTCCAGAAATGGTTTTGATACTGATTCCAGCTGT40.1469507714915503No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC40.1469507714915503No Hit
CTCTTATTTTCTTCAGAATTCCATAATTTTTGTGTATTGTGTGAAAGGCT40.1469507714915503No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA40.1469507714915503No Hit
GTTGTTACACTCGGCCACAAATAAACACAGAAATAGTCCAGAATGTCACA40.1469507714915503No Hit
CACCAGGTGGGCTTCACGATGTACACATCCCAACAGCTGCTGACCAGCTT40.1469507714915503No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG40.1469507714915503No Hit
TAATGAGCATAATAATCGAATTGAAGCCCAGGAAAATTATATTCCAGATC30.11021307861866275No Hit
CTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC30.11021307861866275No Hit
GTACATGGGTGGGGGAAGCGGCGGCAGGCGCCATGTCCGGCCGCGAAGGT30.11021307861866275No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC30.11021307861866275No Hit
GCTCAGAGTAGTTTGATCATCTGAAGCCTTCTTCTCTCAACTCGTCAACG30.11021307861866275No Hit
CCTGAGGAGTATAGGGAGGGACCCTCAGTGCCTCAGTCCTTACCCTCAGG30.11021307861866275No Hit
GGTCAGGCCTCCTGCTCCTGCCAGGGAGCACGCGTGGTCTTCGGGTTGAG30.11021307861866275No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.11021307861866275Clontech Universal Primer Mix Long (96% over 26bp)
ATATGGGGAAATTAAAAACATTCATCTCAACCTCGACAGGCGAACAGGAT30.11021307861866275No Hit
CACTGAAGGTGCCAGCCTATGGGTGGACTCGTGATCTAGACCTAAAGGGT30.11021307861866275No Hit
CACCAACATTGGCCTTTGCAGTCCCCCTGACTTTCTTCATTCTGTTCTTG30.11021307861866275No Hit
AACTTACCCTACACAACTATCTACAGATATATGTTGCCCATGTACTCTGC30.11021307861866275No Hit
TATCAACGCAGAGTACATGGGCGCGACCTCAGATCAGACGTGGCGACCCG30.11021307861866275No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA30.11021307861866275No Hit
AAATTTAAGTCAGTCCTCAAAAATTCATCTTCATATTCTCAAAGTCCCTA30.11021307861866275No Hit
TTCCAATGCTGTCTGGAATCAATTTATTGACCACTTCTTTCAAGTCATTT30.11021307861866275No Hit
TCACTACACTCACTGTGAAAAGAAGCTTAGATGTCAGGGGATTCTGTCGT30.11021307861866275No Hit
GCTTTATTTAAAGGAGCATCTCAGTTACGTGTGGCGGACAAAAAAAAGGA30.11021307861866275No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT30.11021307861866275No Hit
CTAAAATAGTAAATGAAATTTCTTGGAAGTTCAAAAAAAAGATCTTTCAA30.11021307861866275No Hit
CTTTAATGGTTCCAGAGAGTTCTCTGGCTAAGGATCGGTGCCGCATCTGT30.11021307861866275No Hit
CCTTGGATCCTGTCAACATTGGGAGATGGTATAATGGAGGTGTGTCATCT30.11021307861866275No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC30.11021307861866275No Hit
GTATTGAATTGTGAAACTGTGACCAGTAGGCACCAATAGGATTGTGTCTC30.11021307861866275No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG30.11021307861866275No Hit
GTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCTGCCCGCCTT30.11021307861866275No Hit
AGTCCAACCTGAGCGTCCGAGCGAGACTCTATCTCAGAAAATAAAGACAG30.11021307861866275No Hit
ACGCAGAGTACATGGGATAGGCAGAGCGGGTGTCAGACTGAGGATTTCGC30.11021307861866275No Hit
CCCTCGCGACGCGCCCGGCCCGGAGCCCCCAGCGCAGCGGCCGCGTTTGA30.11021307861866275No Hit
AACCACAAATGGTTGATTTATTTCTGACTCTCAGCCCGTCTCTCACGAAG30.11021307861866275No Hit
GGGCTAGGCCGGGTCGCCGCCTGACGCGACGCGTCCTCACGGGCGCCTAC30.11021307861866275No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA30.11021307861866275No Hit
GGTATCAACGCAGAGTACATGGGAGTTGCTGCTGCAGCTGAGGTACAGCG30.11021307861866275No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG30.11021307861866275No Hit
GCCCAGTCTGGTATTTTCTTTTAAGTGTAGCTTTCTTTTTGTTGGCAGTC30.11021307861866275No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA30.11021307861866275No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC30.11021307861866275No Hit
GTCGATGCCGTGGGGCTCGCACGACTACTGGATCCTGCTGACCATCGTGG30.11021307861866275No Hit
ATTCTCGTGCCTCAGCCTCCCTAGTAGCTGGAATTAAAGGCATGCACCAC30.11021307861866275No Hit
GTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCAT30.11021307861866275No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC30.11021307861866275No Hit
CATCAGCAGTGACCAGTGTGGCCAAAGTGGTCAGGGTGGCCTCTGGCTCT30.11021307861866275No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA30.11021307861866275No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACCTGTCTCTTATAC30.11021307861866275No Hit
CCTCATGACCATTCTGCTCATCGTCATCCCAGTGCTGATCTTCCAGGCCT30.11021307861866275No Hit
AATCCCAACACTTTGGGAGGCCAAAGCGGGTGGATCACAAAGTCAGGAGT30.11021307861866275No Hit
CCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGA30.11021307861866275No Hit
AGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCG30.11021307861866275No Hit
GCTCAGCGCAACCTCCGGGCTTCTCGCTCTGACTCCAAAAGGGTGAGCAC30.11021307861866275No Hit
GCTCCGAAATCACCACCAAGGACTTAAAGGAGAAGAAGGAAGTTGTGGAA30.11021307861866275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA30.11021307861866275No Hit
CCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGG30.11021307861866275No Hit
TTCTTGGCTCTCTGCAAGCTCTGCCTCCCGGGTTCTCGCCATTCTCCTGC30.11021307861866275No Hit
CTGCAGAATCCTGCCCCTTCCACCCCAAGGGGCACAGCCCGGAGATGAAG30.11021307861866275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)