FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003671194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT70.2571638501102131No Hit
GGGCAGGGAGCTAGTAGAACCTCGCAATGACAGCCGCAATGGCAGACCCA50.18368846436443792No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG50.18368846436443792No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT50.18368846436443792No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.1469507714915503Clontech Universal Primer Mix Long (96% over 26bp)
GTGCACGCAGGTGCGCGTGGAATTCATGGACGACACGAGCCGATCCATCA40.1469507714915503No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG40.1469507714915503No Hit
GTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC40.1469507714915503No Hit
CCCATTGGCAATGGCCGCCGCGGACATCATCTTGGCTGCTATGGAGGACG40.1469507714915503No Hit
AGACTTTCTACCACACTGTGGGAAGCATCGATAAACAGTCATAATAATTA40.1469507714915503No Hit
GCCAACGTGGGCGCCTCCCTGGGGCCACAGGGTCCAGGGAGAGGTGGGCC40.1469507714915503No Hit
GTATCAACGCAGAGTACATGGGAACCCGGGGACTAACGGCGCCGGTGACG40.1469507714915503No Hit
ATTGAGGGAAGAAAGGGACTTTAGCCTTTCACACAATACACAAAAATTAT40.1469507714915503No Hit
AGTAGATCCACTAGCACATGTGGTTTATCATAACCAAAGGAGGTGGGGGC40.1469507714915503No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA40.1469507714915503No Hit
CAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCA40.1469507714915503No Hit
ATCTGGGCCTGTCTGTTCTGAATGGTCAGTTTCACTGTAATCCTCAGGCC30.11021307861866275No Hit
CCTTTGAGAAGAGACTGGAGGGATTCTTGGCAGGGGAGACCAACTGGTAA30.11021307861866275No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC30.11021307861866275No Hit
GTCTAGTCATCCTCGTCGGTCTTCTGCTTCTTGGTATCGACATCGTCATC30.11021307861866275No Hit
GTGGAGCCCAGATTCAGAGACTGGCCTTGCTGACTCTGAAGACCCTGTTT30.11021307861866275No Hit
AGGGCGAGGAATGGAAGTTGGAGTACGTGGGATACAGGTCATGGGCAGAG30.11021307861866275No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG30.11021307861866275No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT30.11021307861866275No Hit
CTCTGCCTCCCGGGTTCAAGTGATTCTTCTGCCTCAGCCTCCTGAGTAGC30.11021307861866275No Hit
GGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATC30.11021307861866275No Hit
CTCAGAACCTTTGGAATCCCACTTATCGCTCCTGAGCCCCAAGGGGCCCT30.11021307861866275No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTACCTT30.11021307861866275No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC30.11021307861866275No Hit
GTACATGGGCGAGGCTGCGGTGTCTGCTGCTATTCTCCGAGCTTCGCAAT30.11021307861866275No Hit
AGCGAATGCGCAGGCTGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCC30.11021307861866275No Hit
TCCTTAAATTGCCTCTGAGTAAGTAATCTGCAACTGGCCCTGCATTTGAG30.11021307861866275No Hit
GCACCAGGCCCAGCACGGTGGCGGCCACCGCGGTGATCAGGTAGCGCCGC30.11021307861866275No Hit
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCC30.11021307861866275No Hit
CAGTTGCTCCCAGTGACTGCAGAGTAGCCACAAGGCTGCCCGAGGCAACT30.11021307861866275No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC30.11021307861866275No Hit
AGGTAACTGTGAATCAGAAATAGATGAACAGACCCAAGGTGACCTCCTAA30.11021307861866275No Hit
ACGCAGAGTACATGGGAGGCTGAGGCAGGAGAATCACTTGAAACCAGGAG30.11021307861866275No Hit
GACATTGCCAAGGCAACGGGTGACTGGAAGGGCCTGAGGATTACAGTGAA30.11021307861866275No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC30.11021307861866275No Hit
GTACATGGGGTAGAAGAAGAGACGATGAGGCAGAAGTTCCAGAGAGAAAA30.11021307861866275No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT30.11021307861866275No Hit
ACCCTAGCTAAAATGAGTAAAAAGCAAACGACACTGGAGAGCTTCTTTGA30.11021307861866275No Hit
TGCCTTGACAGATGAAGTGATCATTCCTCATTCTGGTTGGGAGCGGGAAC30.11021307861866275No Hit
GAATTATCCTGGTCTTCTTGTTGTCACGCGCGGCGTTGCCAGCCAGCTCC30.11021307861866275No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA30.11021307861866275No Hit
CCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATT30.11021307861866275No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG30.11021307861866275No Hit
CTTGAGGTGTCAGTGGACGTAATCTTAAGGCTGAAAAGTTAGGGAGGAAT30.11021307861866275No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA30.11021307861866275No Hit
CCATGGGACTGGGTCGTCTGAAGGACATAGCCCTGGGGATGCAGACAGAA30.11021307861866275No Hit
TTGTTGCACAGGCTGGAGTGCAATGGTGCAATCTCAGCTCACTGCAACCT30.11021307861866275No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.11021307861866275Clontech SMART CDS Primer II A (96% over 26bp)
GTACACCTGCGAGTGTTCCAATCTCTCCCTCATGAAAACAAGCCCTTGAC30.11021307861866275No Hit
GTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGACTGTCTCTTATAC30.11021307861866275No Hit
TTGTGTTACTTTCTGTTCTGTTTCATTTGTTTTTGAAATATGTTTAGCAA30.11021307861866275No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT30.11021307861866275No Hit
ACAGAGGACCTGTCAGCGACGTCTCCGGTCTGGACTTCTGCTGCGTCTTC30.11021307861866275No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA30.11021307861866275No Hit
TATCAACGCAGAGTACATGGGCCACCACTACCTGTCCCAAGTTCTGCAGG30.11021307861866275No Hit
CTTCCATGGCATGGATCTTACCCGTGACAAAATGTGTTCCATGGTCAAAA30.11021307861866275No Hit
TTCACATACTGGTTCATGAGTGATGTCTTCCCGACTCCAGAATCTCCCAG30.11021307861866275No Hit
AGCAGATTCTTAGCCTTCCCACTCCCGCCCTCTCTCAAGCTCCGGTGCCC30.11021307861866275No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT30.11021307861866275No Hit
GAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCG30.11021307861866275No Hit
CTACCATTTTAGGCTGGGTGCAGTGGCTCACGCCTGTAATCCCAGTACTT30.11021307861866275No Hit
GTACAAGACCACACCGGATGTCATCTTTGTATTTGGATTCAGAACTCATT30.11021307861866275No Hit
GTATCAACGCAGAGTACATGGGGCAGCTTCTCGCTCTCGCCTGCCTGCCC30.11021307861866275No Hit
ACATGGGTCTCCGCCAGACCGCCGCCGCGCCGCCATCATGGACACCAGCC30.11021307861866275No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC30.11021307861866275No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT30.11021307861866275No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT30.11021307861866275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)