FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003671195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG50.17959770114942528No Hit
AAGCTATTCCGTATCTGTCTGGAAACAAACAAAAATTCCCCAAACAAAAA50.17959770114942528No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG40.14367816091954022No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.14367816091954022Clontech Universal Primer Mix Long (96% over 26bp)
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA40.14367816091954022No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC40.14367816091954022No Hit
ACCCTGCTCCCCCGACGTCCACCGTGATCCACATCCGCAGCGAGACCTCC40.14367816091954022No Hit
CTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTA40.14367816091954022No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG40.14367816091954022No Hit
GTTTATGAGCAAGGTGGGTCTCAGAGGTGATCGGCGATCAGAGGGCGATG40.14367816091954022No Hit
CTGTTAACGTGGTTCATGACTTTCTGTTTAAGCTGTGCCACCTGTTCCCT40.14367816091954022No Hit
GTATAAATACTGGCTCTAAGCTTTCATCACTTCATTGACAAACTGAATGC40.14367816091954022No Hit
GCCCTCCTGGGGAGACGTGACTCTGGTGGACGCAGAGCACTTAGTTACCC40.14367816091954022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.14367816091954022No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC40.14367816091954022No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG30.10775862068965517No Hit
ATGTTGGCCAGGCTGGTCTTAAACTCCTGACCTTGCGATCAATCCACCTC30.10775862068965517No Hit
GATGAACACACAACTAACGCCATCCTAAACAGGGAAAAGCTGAAAGCCTT30.10775862068965517No Hit
GTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGGGTGGTGGT30.10775862068965517No Hit
ATTAAGTTACTAACCCAAAATCAAGAATGTTACTTAATTGAGGCCGGGCA30.10775862068965517No Hit
CTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCGCGCCACGATGCCCGGC30.10775862068965517No Hit
ACTTTCTACCACTCCTTTAAATTTGTTTTCTTGGCTGGTATTTTCTTGCC30.10775862068965517No Hit
ATCAACGCAGAGTACATGGGAGTTGCTGCTGCAGCTGAGGTACAGCGGCG30.10775862068965517No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT30.10775862068965517No Hit
CTCCTGGCCTCAAGGGACCCACCCGCCTAGGCCTCCCAAAGCGCTGGGAT30.10775862068965517No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTT30.10775862068965517No Hit
TAACTAGGCTCAAGGCAGGGAAGGAATCCCATTTTTAATCAGTGCTTAAG30.10775862068965517No Hit
TCAACGCAGAGTACATGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGG30.10775862068965517No Hit
CCTCGGGGGCACAGGTGCCTTTGAGATAGAGATAAATGGACAGCTGGTGT30.10775862068965517No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGACCCCGGCCGGGGGGCGGG30.10775862068965517No Hit
CCTCTGTGGTGCGCAGCCCGTACAGCTCCCCGATCAAGGGGGGCACCCAG30.10775862068965517No Hit
GGGCTCAAGTGATCCTCCCACCTCAGCCTCCCAAAGGGCTGGGATTATGG30.10775862068965517No Hit
CTCCTGGGTAGCTGGGATTACAGGCGCAGACCACCACGCCCGGCTAATTT30.10775862068965517No Hit
CACAGAGCCCGGAAAGGCTTTCTTCTCTCTAGAACTAGAGCATTTTCTCC30.10775862068965517No Hit
ACCTTAGAACGGAGTGACTCTGGAGACTCGAGCATATGAAGAAGTTCTGA30.10775862068965517No Hit
CTGTAAACAGAGGCGGGATGATGGAAATGTCCTCGTTATTCCTCTGAGCC30.10775862068965517No Hit
GGTGTAGCCAGACCTCTCTGAGAGACATGGATATCACAGGATGAAGGTAG30.10775862068965517No Hit
GGCGTGATCTTGGTTCACCACCACCTCCGCCTCCTGGGTTCAAGCGATTC30.10775862068965517No Hit
CACGCAGGCACCTATGAGGTTAGATTCTTCGACGAGGAGTCCTACAGCCT30.10775862068965517No Hit
AAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCT30.10775862068965517No Hit
GCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGAC30.10775862068965517No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT30.10775862068965517No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT30.10775862068965517No Hit
CTGCAAACTCTGCCTTCTGGGTTCAAGCGATTCTTGTGCCTCAGCCTCCT30.10775862068965517No Hit
TCGTGAGCCCCACCGACTGGTCGCTGCGCCCCTTCTTCACGCTCTGGAGC30.10775862068965517No Hit
TCCTTGAGCAGATGGGGCAACTGCTGGGTAACCAGCTCTTTGAACTCGTT30.10775862068965517No Hit
TCCCAACAGCTGCTGACCAGCTTCCGATCCTCGCAAGCCCCCTCTGCGGC30.10775862068965517No Hit
ATACAGGACTCGGCTCCGGGGGCAGGGCGAGGAATGGAAGTTGGAGTACG30.10775862068965517No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA30.10775862068965517No Hit
GTTCTCTTCTCTGCAGGGCGAGGGGCAGGGAGCTAGTAGAACCTCGCAAT30.10775862068965517No Hit
GTGGCTGGGAGGGAGAGACCCCCTCGGGTGGCCACACTGAGCTGGAGGGA30.10775862068965517No Hit
TCGTTATGGCCGTCGCCGGAGCAGCTGCGGGAGCTGGAGGCCGAAGAACG30.10775862068965517No Hit
GCATACCACTTTCCGGCGATGGCCCAGAGCAATCCAGAACCAATGAAGTA30.10775862068965517No Hit
GGGCGGCCACCGCGCTGCTCATGGTAGGCGGCTGCCTCTTGTGCTGCGGC30.10775862068965517No Hit
CTCCTGGCCTCAGTGATGCCTCCTGATCTATCCATAGGCCTGGAAGATCA30.10775862068965517No Hit
CTCTCTCTCTGCTGACAATATCTCGGGTCACATCTACCTCAGCATCCACA30.10775862068965517No Hit
ACCATGAAAAGGGCCTTTGCTGGGGGAGCCAACTCCCTGAGGGCGGCGTC30.10775862068965517No Hit
GTGAGGGACGGTGCGGTGGGCTCCATGGTGGAGGCGGGAGATGGCGCTTC30.10775862068965517No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC30.10775862068965517No Hit
AGCTAGTAGAACCTCGCAATGACAGCCGCAATGGCAGACCCAATGGAGCC30.10775862068965517No Hit
GCTAAGGACCGGAAGCCTGGAACTTGGAGAGCTGAGGCAACAGAGAGGCT30.10775862068965517No Hit
ATTCAGGATCTTCCGATGACATTGATTGCTGCATTCCGCTCAACGCTTGA30.10775862068965517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)