FastQCFastQC Report
Tue 10 May 2022
EGAF00003671568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697930
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16220.23240153023942228No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15720.22523748800022925No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT15680.2246643646210938No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15200.21778688407146848No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14440.20689753986789508No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13980.20030662100783747No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG13900.1991603742495666No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13800.19772756580172796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11860.16993108191365897No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11260.16133423122662732No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC10600.1518776954708925No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10220.1464330233691058No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC9710.13912570028512888No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT9650.1382660152164257No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA9580.1372630493029387No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9180.13153181551158424No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT9110.13052884959809724No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9030.12938260283982633No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8990.1288094794606909No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC8820.12637370509936527No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG8740.12522745834109436No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG8590.12307824566933646No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC8580.1229349648245526No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC8450.1210723138423624No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC8310.11906638201538836No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT8310.11906638201538836No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8210.11763357356754975No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT8040.11519779920622411No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG7970.11419483329273708No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC7970.11419483329273708No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC7870.11276202484489849No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7760.111185935552276No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC7570.10846359950138265No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG7550.10817703781181494No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG7410.10617110598484089No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT7240.10373533162351527No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7240.10373533162351527No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7130.10215924233089278No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7050.10101299557262189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)