FastQCFastQC Report
Tue 10 May 2022
EGAF00003671958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884165
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG80520.910689746823274No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG64880.7337996867100597No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG50010.5656184083287621No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC47130.5330453026301651No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA34480.38997245989153606No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT32020.3621495987739845No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC31910.3609054870979964No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT31170.35253600855044026No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA29690.3357970514553279No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG28380.32098081240492443No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT25940.29338415341027974No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA25450.28784220139906014No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG19040.21534442100739115No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC19010.21500511782303078No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC18710.2116120859794269No Hit
GTACATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATAT18080.20448671910785884No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT17080.19317661296251265No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG16950.19170629916361764No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA16500.18661675139821188No Hit
ACCTTACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCT16250.18378922486187532No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC16000.1809616983255388No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC15920.1800568898339111No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG15730.17790796966629532No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA15600.17643765586740032No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA15440.17462803888414494No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG15300.17304462402379645No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC15270.17270532083943607No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTCAGCCT14630.16546685290641452No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC14420.16309173061589183No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA14360.16241312424717108No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC14250.161169012571183No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA13890.15709737435885834No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC13810.15619256586723065No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG13370.15121611916327835No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC12950.14646587458223295No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC12700.14363834804589642No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG12580.14228113530845488No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC12520.1416025289397341No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC12460.14092392257101333No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC12300.13911430558775792No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG12290.1390012045263045No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC12110.13696538542014217No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA11830.13379855569944524No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG11780.13323305039217795No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT11680.13210203977764332No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG11440.12938761430276025No Hit
GTGGTAAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAG11020.12463736972171484No Hit
CTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCT10960.12395876335299406No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA10730.12135743893956444No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT10590.11977402407921597No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC10140.11468447631381021No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT10110.11434517312944982No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAC10000.11310106145346174No Hit
TCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTAC9900.11197005083892711No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC9840.11129144447020636No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG9690.10959492854840443No Hit
AGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA9600.10857701899532327No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG9590.10846391793386981No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT9580.10835081687241635No Hit
TCATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTT9320.10541018927462634No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA9280.10495778502881249No Hit
CAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAG9140.10337437016846403No Hit
GCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGA9100.10292196592265018No Hit
GTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGT9090.10280886486119672No Hit
GTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGT8930.10099924787794134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.10009443938631364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)