FastQCFastQC Report
Tue 10 May 2022
EGAF00003671975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140096
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA3510.2505424851530379No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC2430.17345248972133395No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT1930.137762677021471No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA1900.1356212882594792No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC1820.12991091822750114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.12491434444952033No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC1690.12063156692553678No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1610.1149211968935587No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1560.1113522156235724No Hit
TATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCATCAG1450.10350045682960257No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT1420.10135906806761079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)