FastQCFastQC Report
Tue 10 May 2022
EGAF00003671983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC650.6378802747791953No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT560.549558390578999No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC430.42198233562316No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG410.4023552502453386No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT360.35328753680078506No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG320.3140333660451423No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA310.3042198233562316No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT310.3042198233562316No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC300.2944062806673209No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT290.2845927379784102No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT290.2845927379784102No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT290.2845927379784102No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT270.2649656526005888No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC270.2649656526005888No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC270.2649656526005888No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG240.23552502453385674No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC230.22571148184494602No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC230.22571148184494602No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG230.22571148184494602No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA220.21589793915603533No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC210.20608439646712462No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG210.20608439646712462No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA210.20608439646712462No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT210.20608439646712462No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG200.19627085377821393No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA200.19627085377821393No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC200.19627085377821393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.19627085377821393No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT190.18645731108930325No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC190.18645731108930325No Hit
GTACATGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGC190.18645731108930325No Hit
GTATCAACGCAGAGTACATGGGTCGCCATAGAAGCCGATACCGTGAAAGA190.18645731108930325No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA190.18645731108930325No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT180.17664376840039253No Hit
GTACATGGGTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACG180.17664376840039253No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG170.16683022571148184No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT170.16683022571148184No Hit
GCCATATGCTGTATATTCTTGCGTTAACGGTTTCCGCGTGCGATCTCTCT170.16683022571148184No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG170.16683022571148184No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT170.16683022571148184No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA170.16683022571148184No Hit
CTCTTAAGCAGCGCACTTCTGCATAACTATGCCATACAGTTAGGTGCTAC160.15701668302257116No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG160.15701668302257116No Hit
GTACATGGGTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGA160.15701668302257116No Hit
GTACATGGGTCGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACT160.15701668302257116No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA160.15701668302257116No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA160.15701668302257116No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG160.15701668302257116No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA160.15701668302257116No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA150.14720314033366044No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT150.14720314033366044No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT150.14720314033366044No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC150.14720314033366044No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG150.14720314033366044No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC150.14720314033366044No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT150.14720314033366044No Hit
GCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCA150.14720314033366044No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA150.14720314033366044No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG150.14720314033366044No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA150.14720314033366044No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA150.14720314033366044No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG150.14720314033366044No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG140.13738959764474976No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC140.13738959764474976No Hit
ATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTT140.13738959764474976No Hit
AGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCT140.13738959764474976No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT140.13738959764474976No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC140.13738959764474976No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA140.13738959764474976No Hit
GTATATTCTTGCGTTAACGGTTTCCGCGTGCGATCTCTCTTGTGTTCAGA140.13738959764474976No Hit
CTCTTGAACCGTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAA140.13738959764474976No Hit
ATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATT140.13738959764474976No Hit
GTACATGGGGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTT140.13738959764474976No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG130.12757605495583907No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA130.12757605495583907No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT130.12757605495583907No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG130.12757605495583907No Hit
ATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCA130.12757605495583907No Hit
ACGCAGAGTACATGGGTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAG130.12757605495583907No Hit
GCTATGATGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA130.12757605495583907No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA130.12757605495583907No Hit
CCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGC130.12757605495583907No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT120.11776251226692837No Hit
GTACATGGGAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAG120.11776251226692837No Hit
TATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTT120.11776251226692837No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA120.11776251226692837No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA120.11776251226692837No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA120.11776251226692837No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA120.11776251226692837No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG120.11776251226692837No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG120.11776251226692837No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA110.10794896957801767No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT110.10794896957801767No Hit
TATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGC110.10794896957801767No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA110.10794896957801767No Hit
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC110.10794896957801767No Hit
GCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAGCAAAT110.10794896957801767No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC110.10794896957801767No Hit
GGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTC110.10794896957801767No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC110.10794896957801767No Hit
GTATCAACGCAGAGTACATGGGTCAGCTGCAATATGCACTGCCGCGTCTG110.10794896957801767No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC110.10794896957801767No Hit
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA110.10794896957801767No Hit
CATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCC110.10794896957801767No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC110.10794896957801767No Hit
GTATCAACGCAGAGTACATGGGTATCGCGACTCTTGGTGAGAATGCGAGT110.10794896957801767No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG110.10794896957801767No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC110.10794896957801767No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA110.10794896957801767No Hit
GTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCATTTCAAGAA110.10794896957801767No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT110.10794896957801767No Hit
GTACATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC110.10794896957801767No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT110.10794896957801767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)