FastQCFastQC Report
Tue 10 May 2022
EGAF00003671984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003671984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC730.7163886162904809No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT580.5691854759568205No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT500.49067713444553485No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC480.47105004906771347No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC360.35328753680078506No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC360.35328753680078506No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC320.3140333660451423No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG310.3042198233562316No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC310.3042198233562316No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT310.3042198233562316No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG300.2944062806673209No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT290.2845927379784102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.2845927379784102No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT270.2649656526005888No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC270.2649656526005888No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA250.24533856722276742No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG250.24533856722276742No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA250.24533856722276742No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG240.23552502453385674No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT230.22571148184494602No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC220.21589793915603533No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT210.20608439646712462No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG210.20608439646712462No Hit
GTACATGGGTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACG210.20608439646712462No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT210.20608439646712462No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT200.19627085377821393No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC200.19627085377821393No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA200.19627085377821393No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC200.19627085377821393No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA200.19627085377821393No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT200.19627085377821393No Hit
GTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCATTTCAAGAA200.19627085377821393No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT190.18645731108930325No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA190.18645731108930325No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC190.18645731108930325No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG190.18645731108930325No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT190.18645731108930325No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT180.17664376840039253No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG180.17664376840039253No Hit
GTACATGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGC180.17664376840039253No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA180.17664376840039253No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG170.16683022571148184No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA170.16683022571148184No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG170.16683022571148184No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG170.16683022571148184No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC170.16683022571148184No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG170.16683022571148184No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA170.16683022571148184No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA160.15701668302257116No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG160.15701668302257116No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC160.15701668302257116No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG160.15701668302257116No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA160.15701668302257116No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC150.14720314033366044No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA150.14720314033366044No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA150.14720314033366044No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG150.14720314033366044No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT150.14720314033366044No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG150.14720314033366044No Hit
ATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCA150.14720314033366044No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT150.14720314033366044No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA140.13738959764474976No Hit
CCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATT140.13738959764474976No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT140.13738959764474976No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA140.13738959764474976No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC140.13738959764474976No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA140.13738959764474976No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG140.13738959764474976No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA140.13738959764474976No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT130.12757605495583907No Hit
GTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATG130.12757605495583907No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC130.12757605495583907No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA130.12757605495583907No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA130.12757605495583907No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC130.12757605495583907No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG130.12757605495583907No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC130.12757605495583907No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT130.12757605495583907No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC130.12757605495583907No Hit
GGCTATATTTGGTCCGGGTTGTTATCGTCGTGGTTACCGTAATACTTCAG130.12757605495583907No Hit
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG130.12757605495583907No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT130.12757605495583907No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA130.12757605495583907No Hit
ATTCTATCTATACGCCAGTTTCCCGTGTATCTTATCAGGTAGAGAACACT120.11776251226692837No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT120.11776251226692837No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA120.11776251226692837No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT120.11776251226692837No Hit
GTACATGGGTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGA120.11776251226692837No Hit
GTACATGGGTCGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACT120.11776251226692837No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA120.11776251226692837No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA120.11776251226692837No Hit
GTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAACAGAAAGATC120.11776251226692837No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC120.11776251226692837No Hit
CCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCT120.11776251226692837No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA120.11776251226692837No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG120.11776251226692837No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA120.11776251226692837No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA120.11776251226692837No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA120.11776251226692837No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT110.10794896957801767No Hit
TCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATC110.10794896957801767No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC110.10794896957801767No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC110.10794896957801767No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC110.10794896957801767No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT110.10794896957801767No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA110.10794896957801767No Hit
GAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCG110.10794896957801767No Hit
AGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCT110.10794896957801767No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC110.10794896957801767No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA110.10794896957801767No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG110.10794896957801767No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG110.10794896957801767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA110.10794896957801767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)