FastQCFastQC Report
Tue 10 May 2022
EGAF00003672043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5653
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591.04369361401026No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA280.4953122235980895No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG270.47762250132672923No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC260.4599327790553688No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT260.4599327790553688No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA260.4599327790553688No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC260.4599327790553688No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG260.4599327790553688No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA240.42455333451264815No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT240.42455333451264815No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG220.38917388996992747No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC220.38917388996992747No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT220.38917388996992747No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG190.33610472315584644No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC180.3184150008844861No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT170.3007252786131258No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC170.3007252786131258No Hit
GTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAG170.3007252786131258No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC160.2830355563417654No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA150.2653458340704051No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG150.2653458340704051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.2653458340704051No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT140.24765611179904476No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA140.24765611179904476No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT140.24765611179904476No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG140.24765611179904476No Hit
GTATCAACGCAGAGTACATGGGACTCTGTCTCAGGGGATCTGCATATGTT140.24765611179904476No Hit
CTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGT140.24765611179904476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.24765611179904476No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA130.2299663895276844No Hit
GGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCG130.2299663895276844No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA130.2299663895276844No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT130.2299663895276844No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT130.2299663895276844No Hit
GTACATGGGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTTT130.2299663895276844No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG130.2299663895276844No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA130.2299663895276844No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG130.2299663895276844No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA130.2299663895276844No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC120.21227666725632408No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC120.21227666725632408No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT120.21227666725632408No Hit
GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA120.21227666725632408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.21227666725632408No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC120.21227666725632408No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT110.19458694498496373No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT110.19458694498496373No Hit
GTACATGGGTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAA110.19458694498496373No Hit
GCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGC110.19458694498496373No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG110.19458694498496373No Hit
CTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGC110.19458694498496373No Hit
GTACATGGGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTA110.19458694498496373No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG100.1768972227136034No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA100.1768972227136034No Hit
GGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTT100.1768972227136034No Hit
GTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTA100.1768972227136034No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA100.1768972227136034No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT100.1768972227136034No Hit
ACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAG100.1768972227136034No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG100.1768972227136034No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA100.1768972227136034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.1768972227136034No Hit
TTCTTTGGTCGCAATCCTGCAATCAACGCCGCAACTTTACGTCGGATTAG100.1768972227136034No Hit
TATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATAC100.1768972227136034No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA100.1768972227136034No Hit
CATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAAT100.1768972227136034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA100.1768972227136034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG100.1768972227136034No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA90.15920750044224305No Hit
CGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGA90.15920750044224305No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT90.15920750044224305No Hit
TACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACA90.15920750044224305No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC90.15920750044224305No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC90.15920750044224305No Hit
TCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTC90.15920750044224305No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT90.15920750044224305No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT90.15920750044224305No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC90.15920750044224305No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG90.15920750044224305No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA80.1415177781708827No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC80.1415177781708827No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT80.1415177781708827No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1415177781708827No Hit
GTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGA80.1415177781708827No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC80.1415177781708827No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT80.1415177781708827No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1415177781708827No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA80.1415177781708827No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG80.1415177781708827No Hit
CTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATC80.1415177781708827No Hit
CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTA80.1415177781708827No Hit
CTATGATAAACTTACACTTGTTTGGACTGATGGAAGCACTGGACCGAAAG80.1415177781708827No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA80.1415177781708827No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC80.1415177781708827No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA80.1415177781708827No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG80.1415177781708827No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG80.1415177781708827No Hit
CCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGAT70.12382805589952238No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC70.12382805589952238No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC70.12382805589952238No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG70.12382805589952238No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC70.12382805589952238No Hit
GATGGAAGCACTGGACCGAAAGAAGCAATTGCGCTTGGTTCAAAGATTTT70.12382805589952238No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT70.12382805589952238No Hit
GTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGA70.12382805589952238No Hit
GTATCAACGCAGAGTACATGGGAAGCGGCTACAGTGTTCTTCTTTCAGAT70.12382805589952238No Hit
CCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTG70.12382805589952238No Hit
GAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGT70.12382805589952238No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC70.12382805589952238No Hit
ACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAG70.12382805589952238No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC70.12382805589952238No Hit
CTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTC70.12382805589952238No Hit
CTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTA70.12382805589952238No Hit
ACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCC70.12382805589952238No Hit
CTCTTGAACCGTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAA70.12382805589952238No Hit
GACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTT70.12382805589952238No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA70.12382805589952238No Hit
AATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCC70.12382805589952238No Hit
AATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGA70.12382805589952238No Hit
GCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAA70.12382805589952238No Hit
CAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTA70.12382805589952238No Hit
GATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCAC70.12382805589952238No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA70.12382805589952238No Hit
ACATGGCAGTTGGCCCACATGGAATATCGGTTTGAGGCCCCGAGCTGGAG70.12382805589952238No Hit
CACAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAG70.12382805589952238No Hit
ACATGGGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAG70.12382805589952238No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG70.12382805589952238No Hit
AGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGT70.12382805589952238No Hit
GGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGATAGCGACTGGACAC70.12382805589952238No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG70.12382805589952238No Hit
ATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAAAAG60.10613833362816204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT60.10613833362816204No Hit
CCCTAATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAG60.10613833362816204No Hit
TGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAG60.10613833362816204No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA60.10613833362816204No Hit
TCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGT60.10613833362816204No Hit
ACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCG60.10613833362816204No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA60.10613833362816204No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT60.10613833362816204No Hit
ATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGT60.10613833362816204No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG60.10613833362816204No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG60.10613833362816204No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT60.10613833362816204No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT60.10613833362816204No Hit
GTACATGGGGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTT60.10613833362816204No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC60.10613833362816204No Hit
AATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGAC60.10613833362816204No Hit
GGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGA60.10613833362816204No Hit
AGGGAAACTAGTCAATCGTCTTTGCGAAGTCCGAGTCCAAGTTCTTCTAG60.10613833362816204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT60.10613833362816204No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC60.10613833362816204No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA60.10613833362816204No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA60.10613833362816204No Hit
TACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGA60.10613833362816204No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA60.10613833362816204No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC60.10613833362816204No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG60.10613833362816204No Hit
TCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTG60.10613833362816204No Hit
AGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAG60.10613833362816204No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT60.10613833362816204No Hit
GATTTAACCTTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGCAAGCC60.10613833362816204No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA60.10613833362816204No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG60.10613833362816204No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA60.10613833362816204No Hit
AAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGTTATT60.10613833362816204No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT60.10613833362816204No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC60.10613833362816204No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)