FastQCFastQC Report
Tue 10 May 2022
EGAF00003672045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5631
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.8524240809802877No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG410.7281122358373291No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG290.5150062155922571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.5150062155922571No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA250.4439708755105665No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT240.42621204049014383No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA230.4084532054697212No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC230.4084532054697212No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT220.3906943704492985No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT210.37293553542887586No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC200.3551767004084532No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT200.3551767004084532No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA200.3551767004084532No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC200.3551767004084532No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT190.33741786538803054No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC190.33741786538803054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.33741786538803054No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT180.31965903036760784No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA170.3019001953471852No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG170.3019001953471852No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC170.3019001953471852No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG170.3019001953471852No Hit
GTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGA160.28414136032676257No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA160.28414136032676257No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA160.28414136032676257No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT150.26638252530633993No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC150.26638252530633993No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA150.26638252530633993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG150.26638252530633993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG150.26638252530633993No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG140.24862369028591727No Hit
GGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCATACAGTAACC140.24862369028591727No Hit
GGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGG140.24862369028591727No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG140.24862369028591727No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG130.23086485526549455No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA130.23086485526549455No Hit
GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA130.23086485526549455No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG130.23086485526549455No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC130.23086485526549455No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT120.21310602024507191No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG120.21310602024507191No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG120.21310602024507191No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG120.21310602024507191No Hit
ACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGG120.21310602024507191No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC110.19534718522464925No Hit
ACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAG110.19534718522464925No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC110.19534718522464925No Hit
GTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGT110.19534718522464925No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC100.1775883502042266No Hit
GTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCATAC100.1775883502042266No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG100.1775883502042266No Hit
GGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTT100.1775883502042266No Hit
ATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGT100.1775883502042266No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT100.1775883502042266No Hit
GTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGG100.1775883502042266No Hit
GTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTA100.1775883502042266No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT100.1775883502042266No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG100.1775883502042266No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA100.1775883502042266No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC100.1775883502042266No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA100.1775883502042266No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT100.1775883502042266No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC100.1775883502042266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG100.1775883502042266No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA90.15982951518380392No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT90.15982951518380392No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA90.15982951518380392No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG90.15982951518380392No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA90.15982951518380392No Hit
GACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTT90.15982951518380392No Hit
TCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTC90.15982951518380392No Hit
GCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAGCAAAT90.15982951518380392No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC90.15982951518380392No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA90.15982951518380392No Hit
GTACATGGGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTA90.15982951518380392No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA80.14207068016338129No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC80.14207068016338129No Hit
GATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAA80.14207068016338129No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT80.14207068016338129No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA80.14207068016338129No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT80.14207068016338129No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC80.14207068016338129No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA80.14207068016338129No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC80.14207068016338129No Hit
GCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGC80.14207068016338129No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC80.14207068016338129No Hit
AATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCC80.14207068016338129No Hit
GTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCATTTCAAGAA80.14207068016338129No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT80.14207068016338129No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT80.14207068016338129No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA80.14207068016338129No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA80.14207068016338129No Hit
TATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATAC80.14207068016338129No Hit
GAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGGAAAACTGCGACTGT80.14207068016338129No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA80.14207068016338129No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG80.14207068016338129No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG80.14207068016338129No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT80.14207068016338129No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA70.12431184514295864No Hit
GGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTA70.12431184514295864No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA70.12431184514295864No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT70.12431184514295864No Hit
CGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGA70.12431184514295864No Hit
GCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGT70.12431184514295864No Hit
CTTTAAGCAGTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCA70.12431184514295864No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT70.12431184514295864No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT70.12431184514295864No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG70.12431184514295864No Hit
GGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAG70.12431184514295864No Hit
TTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTC70.12431184514295864No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA70.12431184514295864No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC70.12431184514295864No Hit
CTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTC70.12431184514295864No Hit
AGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGAC70.12431184514295864No Hit
CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTA70.12431184514295864No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG70.12431184514295864No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT70.12431184514295864No Hit
GAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCG70.12431184514295864No Hit
CTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGT70.12431184514295864No Hit
ACGCAGAGTACATGGGTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGC70.12431184514295864No Hit
CCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGAT60.10655301012253596No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT60.10655301012253596No Hit
GTTGCAAACTATGATAAACTTACACTTGTTTGGACTGATGGAAGCACTGG60.10655301012253596No Hit
CAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGG60.10655301012253596No Hit
GAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAA60.10655301012253596No Hit
ACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCG60.10655301012253596No Hit
GACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGC60.10655301012253596No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGG60.10655301012253596No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA60.10655301012253596No Hit
GTTCTTTAACCAAACATCCGTGCGATTCGTGCCACTCGTAGACGGCATCT60.10655301012253596No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA60.10655301012253596No Hit
GATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAG60.10655301012253596No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG60.10655301012253596No Hit
ACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTA60.10655301012253596No Hit
CCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCA60.10655301012253596No Hit
GTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTAC60.10655301012253596No Hit
GTTTCGTGGCGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACAT60.10655301012253596No Hit
GGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGA60.10655301012253596No Hit
GACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTA60.10655301012253596No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT60.10655301012253596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT60.10655301012253596No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA60.10655301012253596No Hit
CTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATC60.10655301012253596No Hit
GTACATGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATG60.10655301012253596No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG60.10655301012253596No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA60.10655301012253596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT60.10655301012253596No Hit
GATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCA60.10655301012253596No Hit
GTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAG60.10655301012253596No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG60.10655301012253596No Hit
TCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTG60.10655301012253596No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT60.10655301012253596No Hit
AGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGT60.10655301012253596No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA60.10655301012253596No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA60.10655301012253596No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)