FastQCFastQC Report
Tue 10 May 2022
EGAF00003672046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5631
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.8524240809802877No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT360.6393180607352157No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT320.5682827206535251No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA290.5150062155922571No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC270.47948854555141185No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC270.47948854555141185No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG270.47948854555141185No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG260.4617297105309891No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT250.4439708755105665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.4084532054697212No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC220.3906943704492985No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG220.3906943704492985No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC210.37293553542887586No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC200.3551767004084532No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC200.3551767004084532No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA200.3551767004084532No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT190.33741786538803054No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC180.31965903036760784No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG170.3019001953471852No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA170.3019001953471852No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA170.3019001953471852No Hit
GTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGT170.3019001953471852No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG170.3019001953471852No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC160.28414136032676257No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA160.28414136032676257No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC160.28414136032676257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.28414136032676257No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT150.26638252530633993No Hit
GTACATGGGTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAA150.26638252530633993No Hit
GCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGT150.26638252530633993No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.26638252530633993No Hit
CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTA150.26638252530633993No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA140.24862369028591727No Hit
ACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAG140.24862369028591727No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG140.24862369028591727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG140.24862369028591727No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG140.24862369028591727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.23086485526549455No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC130.23086485526549455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.23086485526549455No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA130.23086485526549455No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA130.23086485526549455No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG120.21310602024507191No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT120.21310602024507191No Hit
GTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTA120.21310602024507191No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG120.21310602024507191No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC120.21310602024507191No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT110.19534718522464925No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG110.19534718522464925No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA110.19534718522464925No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT110.19534718522464925No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA110.19534718522464925No Hit
GTACATGGGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTA110.19534718522464925No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA100.1775883502042266No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT100.1775883502042266No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC100.1775883502042266No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC100.1775883502042266No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG100.1775883502042266No Hit
TTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCG100.1775883502042266No Hit
GCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAA100.1775883502042266No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC100.1775883502042266No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA100.1775883502042266No Hit
AACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGGAAAA100.1775883502042266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG100.1775883502042266No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT90.15982951518380392No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG90.15982951518380392No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA90.15982951518380392No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA90.15982951518380392No Hit
GTACATGGGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTTT90.15982951518380392No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA90.15982951518380392No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT90.15982951518380392No Hit
GAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCC90.15982951518380392No Hit
TCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTC90.15982951518380392No Hit
AATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTT90.15982951518380392No Hit
GTATCAACGCAGAGTACATGGGACTCTGTCTCAGGGGATCTGCATATGTT90.15982951518380392No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC90.15982951518380392No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG90.15982951518380392No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT90.15982951518380392No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG90.15982951518380392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA90.15982951518380392No Hit
ACGCAGAGTACATGGGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTA90.15982951518380392No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA80.14207068016338129No Hit
GTACATGGGTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATT80.14207068016338129No Hit
GTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAG80.14207068016338129No Hit
CAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGG80.14207068016338129No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT80.14207068016338129No Hit
GGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACC80.14207068016338129No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.14207068016338129No Hit
GACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTA80.14207068016338129No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT80.14207068016338129No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC80.14207068016338129No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC80.14207068016338129No Hit
CCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAA80.14207068016338129No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT80.14207068016338129No Hit
TCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGC70.12431184514295864No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT70.12431184514295864No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC70.12431184514295864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT70.12431184514295864No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC70.12431184514295864No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA70.12431184514295864No Hit
CCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGA70.12431184514295864No Hit
GATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAA70.12431184514295864No Hit
ATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTA70.12431184514295864No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG70.12431184514295864No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC70.12431184514295864No Hit
GTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGA70.12431184514295864No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA70.12431184514295864No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC70.12431184514295864No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC70.12431184514295864No Hit
CTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTC70.12431184514295864No Hit
CTCTTGAACCGTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAA70.12431184514295864No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.12431184514295864No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG70.12431184514295864No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA70.12431184514295864No Hit
ACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGG70.12431184514295864No Hit
CGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGT70.12431184514295864No Hit
CGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCC70.12431184514295864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG70.12431184514295864No Hit
CCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGAT60.10655301012253596No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA60.10655301012253596No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC60.10655301012253596No Hit
GTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCATAC60.10655301012253596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60.10655301012253596No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG60.10655301012253596No Hit
ACATGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGAT60.10655301012253596No Hit
GGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATC60.10655301012253596No Hit
GTACATGGACCCCAGCAGAAGCCGGTGATGCCGGCCTTGCTGGTGTCGCC60.10655301012253596No Hit
GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA60.10655301012253596No Hit
GTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGA60.10655301012253596No Hit
CCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGG60.10655301012253596No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG60.10655301012253596No Hit
GACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGC60.10655301012253596No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT60.10655301012253596No Hit
GTATCAACGCAGAGTACATGGGTAAGCTCGGCAATCGCTCTTGCCGTGCG60.10655301012253596No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA60.10655301012253596No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT60.10655301012253596No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG60.10655301012253596No Hit
GGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAAC60.10655301012253596No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT60.10655301012253596No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA60.10655301012253596No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG60.10655301012253596No Hit
TCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGT60.10655301012253596No Hit
GACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTT60.10655301012253596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA60.10655301012253596No Hit
CTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGC60.10655301012253596No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG60.10655301012253596No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC60.10655301012253596No Hit
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT60.10655301012253596No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC60.10655301012253596No Hit
GATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCAC60.10655301012253596No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA60.10655301012253596No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT60.10655301012253596No Hit
TATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATAC60.10655301012253596No Hit
GAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCG60.10655301012253596No Hit
CATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAAT60.10655301012253596No Hit
CTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGT60.10655301012253596No Hit
GTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACC60.10655301012253596No Hit
TTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGA60.10655301012253596No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG60.10655301012253596No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA60.10655301012253596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)