FastQCFastQC Report
Tue 10 May 2022
EGAF00003672055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18091
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1320.7296445746503786No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1040.574871483057874No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA860.4753744956055498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.40904317063733353No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT680.37587750815322535No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC670.37034989773920735No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA580.32060140401304515No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG550.3040185727709911No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA520.287435741528937No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA510.281908131114919No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG480.26532529987286496No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG470.25979768945884696No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG420.23215963738875683No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT410.2266320269747388No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA400.22110441656072083No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT390.21557680614670277No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC390.21557680614670277No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG390.21557680614670277No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA390.21557680614670277No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG390.21557680614670277No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC390.21557680614670277No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC380.21004919573268474No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT370.20452158531866677No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG370.20452158531866677No Hit
GTACATGGGTCTGCGGATCAGTCTATCCTCGCTGATGCTCAGTGATACAC370.20452158531866677No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG370.20452158531866677No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT350.1934663644906307No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC350.1934663644906307No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC340.18793875407661267No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC340.18793875407661267No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT340.18793875407661267No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA330.18241114366259464No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA330.18241114366259464No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC330.18241114366259464No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT320.17688353324857664No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC310.17135592283455864No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC310.17135592283455864No Hit
GTCTAGCACTGTAGCTGGTGCATGTACTCGACTGCCGCTGCATCTACTAT310.17135592283455864No Hit
GTCTATCCTCGCTGATGCTCAGTGATACACGCTGATGCACGTAGTGAGCA310.17135592283455864No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT300.16582831242054058No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA300.16582831242054058No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT300.16582831242054058No Hit
GTATACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA290.16030070200652258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.16030070200652258No Hit
CTATAAGACTCTGACATTAGCGTATAGGCTGATACATTAGCTCGGATGCT290.16030070200652258No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG280.15477309159250457No Hit
GGATAGAGTCTGAGACCTCGTGTAGCATGTTGTGGCACCGTGATCCAGTA280.15477309159250457No Hit
GCTATAACAGCGAGTGCTACGCCTAAACTGGCTGTCTAGCACTGTAGCTG270.14924548117848654No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA270.14924548117848654No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC260.1437178707644685No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG260.1437178707644685No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC260.1437178707644685No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC260.1437178707644685No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG260.1437178707644685No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA260.1437178707644685No Hit
ATCGAGCACAGTGTCGGCTATGAGCGCACAGTCTCGTCATATAACGATCA260.1437178707644685No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA250.1381902603504505No Hit
GCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA250.1381902603504505No Hit
GTACATGGGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTG250.1381902603504505No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT250.1381902603504505No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT250.1381902603504505No Hit
GTCTCGTCATATAACGATCACGGCAGTGTAGATCAGAGCATCGGACAGGT250.1381902603504505No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG240.13266264993643248No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT240.13266264993643248No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT240.13266264993643248No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG240.13266264993643248No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG240.13266264993643248No Hit
TCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTC240.13266264993643248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.13266264993643248No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT230.12713503952241445No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT230.12713503952241445No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA230.12713503952241445No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT230.12713503952241445No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA230.12713503952241445No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA230.12713503952241445No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT230.12713503952241445No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG230.12713503952241445No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG220.12160742910839643No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC220.12160742910839643No Hit
GTACATGGGGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAG220.12160742910839643No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA220.12160742910839643No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT220.12160742910839643No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA220.12160742910839643No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG220.12160742910839643No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT220.12160742910839643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.12160742910839643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.12160742910839643No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTATACGAGTGGCACGA210.11607981869437842No Hit
CGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGA210.11607981869437842No Hit
ATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGT210.11607981869437842No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG210.11607981869437842No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT210.11607981869437842No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC210.11607981869437842No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA210.11607981869437842No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG200.11055220828036041No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT200.11055220828036041No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT200.11055220828036041No Hit
CGTATAGGCTGATACATTAGCTCGGATGCTATCAGCTTGCGCCTATTATA200.11055220828036041No Hit
GTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAG200.11055220828036041No Hit
GTGTAGATCAGAGCATCGGACAGGTACACAGATACGCGCTCAGCATAGCA200.11055220828036041No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC200.11055220828036041No Hit
GTCTGAGACCTCGTGTAGCATGTTGTGGCACCGTGATCCAGTATATGAGC200.11055220828036041No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA200.11055220828036041No Hit
GTACATGGGAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGC190.10502459786634237No Hit
GGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGG190.10502459786634237No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC190.10502459786634237No Hit
CTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAA190.10502459786634237No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT190.10502459786634237No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC190.10502459786634237No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA190.10502459786634237No Hit
CGTCCGCACAGCGTGGTCTTATCGAGCACAGTGTCGGCTATGAGCGCACA190.10502459786634237No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA190.10502459786634237No Hit
GTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGA190.10502459786634237No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG190.10502459786634237No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG190.10502459786634237No Hit
CTACGGAGTGTGCTCGGCTATAACAGCGAGTGCTACGCCTAAACTGGCTG190.10502459786634237No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC190.10502459786634237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)