FastQCFastQC Report
Tue 10 May 2022
EGAF00003672056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18091
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1070.5914543142999281No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA880.48642971643358573No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG780.43115361229340554No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT760.4200983914653695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.3979879498092974No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC670.37034989773920735No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT660.3648222873251893No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA650.35929467691117134No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA620.3427118456691173No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT550.3040185727709911No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG500.276380520700901No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC500.276380520700901No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA430.2376872478027749No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC420.23215963738875683No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC420.23215963738875683No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC410.2266320269747388No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC390.21557680614670277No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG390.21557680614670277No Hit
GTACATGGGAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGC380.21004919573268474No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA380.21004919573268474No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG370.20452158531866677No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC360.1989939749046487No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT350.1934663644906307No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA350.1934663644906307No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT350.1934663644906307No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC350.1934663644906307No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT340.18793875407661267No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG340.18793875407661267No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC340.18793875407661267No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG340.18793875407661267No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT330.18241114366259464No Hit
GTACATGGGTCTGCGGATCAGTCTATCCTCGCTGATGCTCAGTGATACAC330.18241114366259464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.18241114366259464No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT320.17688353324857664No Hit
GTATATGAGCTACCGAGTCGTTCTGATAGATCCCGCGTCAGGCATATAAT320.17688353324857664No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC310.17135592283455864No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT310.17135592283455864No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG300.16582831242054058No Hit
GTACATGGGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTG300.16582831242054058No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT300.16582831242054058No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA290.16030070200652258No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA290.16030070200652258No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG280.15477309159250457No Hit
CTATAAGACTCTGACATTAGCGTATAGGCTGATACATTAGCTCGGATGCT280.15477309159250457No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA270.14924548117848654No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG270.14924548117848654No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA270.14924548117848654No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA270.14924548117848654No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT260.1437178707644685No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA260.1437178707644685No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG260.1437178707644685No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG260.1437178707644685No Hit
GTCTAGCACTGTAGCTGGTGCATGTACTCGACTGCCGCTGCATCTACTAT260.1437178707644685No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT250.1381902603504505No Hit
GATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCAC250.1381902603504505No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG250.1381902603504505No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA250.1381902603504505No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT250.1381902603504505No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT250.1381902603504505No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT240.13266264993643248No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG240.13266264993643248No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC240.13266264993643248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.13266264993643248No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC240.13266264993643248No Hit
GCTATAACAGCGAGTGCTACGCCTAAACTGGCTGTCTAGCACTGTAGCTG240.13266264993643248No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA240.13266264993643248No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG240.13266264993643248No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG240.13266264993643248No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA240.13266264993643248No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA240.13266264993643248No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT240.13266264993643248No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC230.12713503952241445No Hit
GTCTTATCGAGCACAGTGTCGGCTATGAGCGCACAGTCTCGTCATATAAC220.12160742910839643No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA220.12160742910839643No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA220.12160742910839643No Hit
GATATGAGCCAGTGCTGCTGCTCTCTACGGAGTGTGCTCGGCTATAACAG220.12160742910839643No Hit
ATCGAGCACAGTGTCGGCTATGAGCGCACAGTCTCGTCATATAACGATCA220.12160742910839643No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT220.12160742910839643No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC220.12160742910839643No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT220.12160742910839643No Hit
GTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGT220.12160742910839643No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG210.11607981869437842No Hit
GTACACAGATACGCGCTCAGCATAGCAGATGCACGTTGAGTCCACGGGAT210.11607981869437842No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA210.11607981869437842No Hit
GTACATGGGACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCT210.11607981869437842No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC210.11607981869437842No Hit
GGATAGAGTCTGAGACCTCGTGTAGCATGTTGTGGCACCGTGATCCAGTA210.11607981869437842No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC210.11607981869437842No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG200.11055220828036041No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG200.11055220828036041No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT200.11055220828036041No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT200.11055220828036041No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG200.11055220828036041No Hit
CCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGC200.11055220828036041No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC200.11055220828036041No Hit
GAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTT200.11055220828036041No Hit
GTCTTATAGTAGATGCAGCGGCAGTCGAGTACATGCACCAGCTACAGTGC200.11055220828036041No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG200.11055220828036041No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC200.11055220828036041No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA190.10502459786634237No Hit
CGTCCGCACAGCGTGGTCTTATCGAGCACAGTGTCGGCTATGAGCGCACA190.10502459786634237No Hit
GCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGC190.10502459786634237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)