FastQCFastQC Report
Tue 10 May 2022
EGAF00003672057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18787
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1090.5801884281684143No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT970.5163144727737266No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA920.4897003246926066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.43647202853036676No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA750.3992122212167989No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC700.37259807313567894No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT690.36727524351945495No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA620.330015436205887No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT540.28743279927609516No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT490.2608186511949752No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA480.2554958215787513No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG460.2448501623463033No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC460.2448501623463033No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA450.2395273327300793No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG440.23420450311385532No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT430.22888167349763133No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG430.22888167349763133No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT410.21823601426518338No Hit
GTACATGGGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTG390.2075903550327354No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC390.2075903550327354No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG380.2022675254165114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.2022675254165114No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA370.19694469580028742No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA360.19162186618406346No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA360.19162186618406346No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC360.19162186618406346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG360.19162186618406346No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG350.18629903656783947No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC350.18629903656783947No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG330.1756533773353915No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA330.1756533773353915No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG310.1650077181029435No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG300.15968488848671955No Hit
GTACATGGGGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAG300.15968488848671955No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA300.15968488848671955No Hit
GTACATGGGTCTGCGGATCAGTCTATCCTCGCTGATGCTCAGTGATACAC300.15968488848671955No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG300.15968488848671955No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC300.15968488848671955No Hit
GCATAGCAGATGCACGTTGAGTCCACGGGATAGAGTCTGAGACCTCGTGT300.15968488848671955No Hit
TCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTC300.15968488848671955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.15436205887049556No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA290.15436205887049556No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG290.15436205887049556No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT290.15436205887049556No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC290.15436205887049556No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC280.14903922925427157No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG280.14903922925427157No Hit
GTACATGGGAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGC270.14371639963804758No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT270.14371639963804758No Hit
GTATATGAGCTACCGAGTCGTTCTGATAGATCCCGCGTCAGGCATATAAT270.14371639963804758No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT260.1383935700218236No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT260.1383935700218236No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA260.1383935700218236No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG260.1383935700218236No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT260.1383935700218236No Hit
GTCTATCCTCGCTGATGCTCAGTGATACACGCTGATGCACGTAGTGAGCA260.1383935700218236No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT250.1330707404055996No Hit
GTATCAACGCAGAGTACATGGGAACGGTGTGCTTCAAATTGCGCTTGTCG250.1330707404055996No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG250.1330707404055996No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA250.1330707404055996No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC250.1330707404055996No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC250.1330707404055996No Hit
GTGATACACGCTGATGCACGTAGTGAGCATCCTGTGCTCGCATATACCGC250.1330707404055996No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT250.1330707404055996No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC240.12774791078937564No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG230.12242508117315165No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA230.12242508117315165No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG230.12242508117315165No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA230.12242508117315165No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG230.12242508117315165No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA230.12242508117315165No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA230.12242508117315165No Hit
GTCTGAGACCTCGTGTAGCATGTTGTGGCACCGTGATCCAGTATATGAGC230.12242508117315165No Hit
CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTA230.12242508117315165No Hit
GGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGG220.11710225155692766No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA220.11710225155692766No Hit
GGTACACAGATACGCGCTCAGCATAGCAGATGCACGTTGAGTCCACGGGA220.11710225155692766No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG220.11710225155692766No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA220.11710225155692766No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG210.11177942194070369No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC210.11177942194070369No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT210.11177942194070369No Hit
ATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTC210.11177942194070369No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA210.11177942194070369No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC210.11177942194070369No Hit
GCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGC210.11177942194070369No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG210.11177942194070369No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC210.11177942194070369No Hit
GTCTCGTCATATAACGATCACGGCAGTGTAGATCAGAGCATCGGACAGGT210.11177942194070369No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC200.1064565923244797No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA200.1064565923244797No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC200.1064565923244797No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA200.1064565923244797No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT200.1064565923244797No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT200.1064565923244797No Hit
GTGTCGGCTATGAGCGCACAGTCTCGTCATATAACGATCACGGCAGTGTA200.1064565923244797No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT200.1064565923244797No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT190.1011337627082557No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT190.1011337627082557No Hit
ATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTT190.1011337627082557No Hit
GGCATATAATAGGCGCAAGCTGATAGCATCCGAGCTAATGTATCAGCCTA190.1011337627082557No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT190.1011337627082557No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT190.1011337627082557No Hit
GTACATGGGAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGA190.1011337627082557No Hit
GATATGAGCCAGTGCTGCTGCTCTCTACGGAGTGTGCTCGGCTATAACAG190.1011337627082557No Hit
TCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATG190.1011337627082557No Hit
GGATAGAGTCTGAGACCTCGTGTAGCATGTTGTGGCACCGTGATCCAGTA190.1011337627082557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)