FastQCFastQC Report
Tue 10 May 2022
EGAF00003672065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5630
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.7460035523978685No Hit
GACCACCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTCGGCTCGTCCA380.6749555950266429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.47957371225577267No Hit
GTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACAAC250.4440497335701598No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT240.42628774422735344No Hit
ACGCAGAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGCATCCG240.42628774422735344No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT200.3552397868561279No Hit
CCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTCGGCTCGTCCAGCAGC200.3552397868561279No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA190.33747779751332146No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG190.33747779751332146No Hit
GTGCCGCGGGTGGCGACCATCGACGTCGTCGCCGAGCCGTTGATCGTCAA180.3197158081705151No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA170.3019538188277087No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA160.2841918294849023No Hit
GTTGTTGACCTTGCGACCGCCGAGCCGCGTCAGGTGCTGAGCATCCGCCG160.2841918294849023No Hit
GACCACAGCCGCTCCGGAATGTTGAGCCGGCGCAGCAGTTTGCCTGCACG160.2841918294849023No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC160.2841918294849023No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT150.2664298401420959No Hit
TCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTC150.2664298401420959No Hit
GTACATGGGTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCA140.24866785079928952No Hit
GTCCGAGATGAATCCGCGTGCAATGTTGACGCGCTGCTGCTCGCCGCCGG140.24866785079928952No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA140.24866785079928952No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA140.24866785079928952No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC140.24866785079928952No Hit
GTATCAACGCAGAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGG130.23090586145648315No Hit
ATGTCAGCCAGTTCCTGCGCGCGGTGCCGCGGGTGGCGACCATCGACGTC130.23090586145648315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.23090586145648315No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT130.23090586145648315No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG130.23090586145648315No Hit
TATCAACGCAGAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGC120.21314387211367672No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG120.21314387211367672No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC120.21314387211367672No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA120.21314387211367672No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT120.21314387211367672No Hit
AGATGAATCCGCGTGCAATGTTGACGCGCTGCTGCTCGCCGCCGGAGAAG120.21314387211367672No Hit
CTCTTAGAGCGTGTATCGCAAAGCACGGTTCTTCCATGGCAAGTACGGGA110.19538188277087032No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG110.19538188277087032No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC110.19538188277087032No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC110.19538188277087032No Hit
CAATCAGCTCGACCACCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTC110.19538188277087032No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG110.19538188277087032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG110.19538188277087032No Hit
GTGCTGAGCATCCGCCGCTCGACGATCGGCTATGTCAGCCAGTTCCTGCG100.17761989342806395No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT100.17761989342806395No Hit
GTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGACTACCCG100.17761989342806395No Hit
GAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACA100.17761989342806395No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG100.17761989342806395No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT100.17761989342806395No Hit
GTCAGGTGCTGAGCATCCGCCGCTCGACGATCGGCTATGTCAGCCAGTTC90.15985790408525755No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT90.15985790408525755No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC90.15985790408525755No Hit
GTACATGGGAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATT90.15985790408525755No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG90.15985790408525755No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA90.15985790408525755No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG90.15985790408525755No Hit
TATCAACGCAGAGTACATGGGAACGCTTGAGGAAGTAAAAGAAGCGGATT90.15985790408525755No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA90.15985790408525755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT90.15985790408525755No Hit
GTCCAGCAGCAGGATCGGCAGGTCCGAGATGAATCCGCGTGCAATGTTGA90.15985790408525755No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA90.15985790408525755No Hit
GATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCAC90.15985790408525755No Hit
CTGCTGCTCGCCGCCGGAGAAGGTCGACGGCGGCAAGGACCACAGCCGCT90.15985790408525755No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG80.14209591474245115No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT80.14209591474245115No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA80.14209591474245115No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT80.14209591474245115No Hit
GGATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACAACGGCAGGG80.14209591474245115No Hit
ACGCAGAGTACATGGGAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATT80.14209591474245115No Hit
GTACATGGGTCTGGTCTCAAATCAGGTAGGATCACCCGCTGAACTTAAGC80.14209591474245115No Hit
GGTTATTATCGTCTCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC80.14209591474245115No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG80.14209591474245115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA80.14209591474245115No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC80.14209591474245115No Hit
GTACATGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCG70.12433392539964476No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG70.12433392539964476No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12433392539964476No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC70.12433392539964476No Hit
GTACATGGGAGACTGGAGGAACAAACAATTGTTTCTTGGAATGCAATCAT70.12433392539964476No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA70.12433392539964476No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA70.12433392539964476No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12433392539964476No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG70.12433392539964476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT70.12433392539964476No Hit
GATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAA70.12433392539964476No Hit
GCTCGACCACCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTCGGCTCG70.12433392539964476No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT70.12433392539964476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG70.12433392539964476No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT70.12433392539964476No Hit
GGTGGCGACCATCGACGTCGTCGCCGAGCCGTTGATCGTCAACGGCGTGG70.12433392539964476No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA70.12433392539964476No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12433392539964476No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT70.12433392539964476No Hit
CATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAAT70.12433392539964476No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT60.10657193605683836No Hit
TCCAGCAGCAGGATCGGCAGGTCCGAGATGAATCCGCGTGCAATGTTGAC60.10657193605683836No Hit
CGACCACCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTCGGCTCGTCC60.10657193605683836No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAAGCGTCTTACAT60.10657193605683836No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT60.10657193605683836No Hit
GCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCA60.10657193605683836No Hit
CCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATT60.10657193605683836No Hit
GCATCCGCCACAACGGCAGGGTTGTTGACCTTGCGACCGCCGAGCCGCGT60.10657193605683836No Hit
AGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGA60.10657193605683836No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA60.10657193605683836No Hit
GGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAAC60.10657193605683836No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTATCCTTAATCTCGTATG60.10657193605683836No Hit
CTCGCGGGCTTCCTCGCGCGCCACGCCGTTGACGATCAACGGCTCGGCGA60.10657193605683836No Hit
CTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTC60.10657193605683836No Hit
GTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAG60.10657193605683836No Hit
ACGATCGGCTATGTCAGCCAGTTCCTGCGCGCGGTGCCGCGGGTGGCGAC60.10657193605683836No Hit
CCGCTCGACGATCGGCTATGTCAGCCAGTTCCTGCGCGCGGTGCCGCGGG60.10657193605683836No Hit
CTGCGACAGCGGCCGGATCGGCATCCGCCACAACGGCAGGGTTGTTGACC60.10657193605683836No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA60.10657193605683836No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA60.10657193605683836No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC60.10657193605683836No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT60.10657193605683836No Hit
ATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCA60.10657193605683836No Hit
ATGTTGACGCGCTGCTGCTCGCCGCCGGAGAAGGTCGACGGCGGCAAGGA60.10657193605683836No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG60.10657193605683836No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA60.10657193605683836No Hit
GTCAGCCAGTTCCTGCGCGCGGTGCCGCGGGTGGCGACCATCGACGTCGT60.10657193605683836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60.10657193605683836No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA60.10657193605683836No Hit
CTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTA60.10657193605683836No Hit
ATCAACGCAGAGTACATGGGTCTGGTCTCAAATCAGGTAGGATCACCCGC60.10657193605683836No Hit
CTGCAGACCCTGCAGAACCAGGTGAACACCAGCGTCACCCAGTTCGTTGG60.10657193605683836No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA60.10657193605683836No Hit
GTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCG60.10657193605683836No Hit
GATCGGCATCCGCCACAACGGCAGGGTTGTTGACCTTGCGACCGCCGAGC60.10657193605683836No Hit
TTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCG60.10657193605683836No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG60.10657193605683836No Hit
CCCATTTTGAGCTGCAATCCCAAACAACTCGACTCTTAGAGCGTGTATCG60.10657193605683836No Hit
GTATCAACGCAGAGTACATGGGTCTGGTCTCAAATCAGGTAGGATCACCC60.10657193605683836No Hit
GGTATCAACGCAGAGTACATGGGAACGCTTGAGGAAGTAAAAGAAGCGGA60.10657193605683836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA60.10657193605683836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)