FastQCFastQC Report
Tue 10 May 2022
EGAF00003672068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.6997900629811057No Hit
GACCACCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTCGGCTCGTCCA310.5423372988103569No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA300.5248425472358292No Hit
GTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACAAC260.45486354093771875No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT250.437368789363191No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT250.437368789363191No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA240.4198740377886634No Hit
GTTGTTGACCTTGCGACCGCCGAGCCGCGTCAGGTGCTGAGCATCCGCCG240.4198740377886634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.3848845346396081No Hit
GATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACAACGGCAGGGT180.31490552834149754No Hit
TATCAACGCAGAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGC170.2974107767669699No Hit
ATGTTGACGCGCTGCTGCTCGCCGCCGGAGAAGGTCGACGGCGGCAAGGA170.2974107767669699No Hit
GACCACAGCCGCTCCGGAATGTTGAGCCGGCGCAGCAGTTTGCCTGCACG170.2974107767669699No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA160.27991602519244224No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.27991602519244224No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT160.27991602519244224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.2624212736179146No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG150.2624212736179146No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA140.244926522043387No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT140.244926522043387No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA140.244926522043387No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC140.244926522043387No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC140.244926522043387No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT130.22743177046885937No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC130.22743177046885937No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG130.22743177046885937No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC130.22743177046885937No Hit
GTATCAACGCAGAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGG120.2099370188943317No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG120.2099370188943317No Hit
CCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTG120.2099370188943317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.2099370188943317No Hit
AGATGAATCCGCGTGCAATGTTGACGCGCTGCTGCTCGCCGCCGGAGAAG120.2099370188943317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.2099370188943317No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.19244226731980404No Hit
ACGCAGAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGCATCCG110.19244226731980404No Hit
GTTGACGCGCTGCTGCTCGCCGCCGGAGAAGGTCGACGGCGGCAAGGACC110.19244226731980404No Hit
GTACATGGGAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATT110.19244226731980404No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT110.19244226731980404No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC110.19244226731980404No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG110.19244226731980404No Hit
GGATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACAACGGCAGGG110.19244226731980404No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA110.19244226731980404No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA110.19244226731980404No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT110.19244226731980404No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA110.19244226731980404No Hit
GTGCCGCGGGTGGCGACCATCGACGTCGTCGCCGAGCCGTTGATCGTCAA100.17494751574527642No Hit
CAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGG100.17494751574527642No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG100.17494751574527642No Hit
CTCCGGAATGTTGAGCCGGCGCAGCAGTTTGCCTGCACGCTCGCGGGCTT100.17494751574527642No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA100.17494751574527642No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG90.15745276417074877No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT90.15745276417074877No Hit
GTCCGAGATGAATCCGCGTGCAATGTTGACGCGCTGCTGCTCGCCGCCGG90.15745276417074877No Hit
CCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTCGGCTCGTCCAGCAGC90.15745276417074877No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG90.15745276417074877No Hit
CAATCAGCTCGACCACCACTGCGCGATTCGCCGCATCGAGCGAAGCGGTC90.15745276417074877No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT90.15745276417074877No Hit
GTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCT80.13995801259622112No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA80.13995801259622112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG80.13995801259622112No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA80.13995801259622112No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA80.13995801259622112No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT80.13995801259622112No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT80.13995801259622112No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA80.13995801259622112No Hit
CCTTGCGACCGCCGAGCCGCGTCAGGTGCTGAGCATCCGCCGCTCGACGA80.13995801259622112No Hit
CTCTTAGAGCGTGTATCGCAAAGCACGGTTCTTCCATGGCAAGTACGGGA70.1224632610216935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG70.1224632610216935No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAAGCGTCTTACAT70.1224632610216935No Hit
ATGTCAGCCAGTTCCTGCGCGCGGTGCCGCGGGTGGCGACCATCGACGTC70.1224632610216935No Hit
GTCAGGTGCTGAGCATCCGCCGCTCGACGATCGGCTATGTCAGCCAGTTC70.1224632610216935No Hit
GCATCCGCCACAACGGCAGGGTTGTTGACCTTGCGACCGCCGAGCCGCGT70.1224632610216935No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA70.1224632610216935No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC70.1224632610216935No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG70.1224632610216935No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG70.1224632610216935No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA70.1224632610216935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT70.1224632610216935No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1224632610216935No Hit
TCGCCGCATCGAGCGAAGCGGTCGGCTCGTCCAGCAGCAGGATCGGCAGG70.1224632610216935No Hit
GAGTACATGGGATTACCGCTGCGACAGCGGCCGGATCGGCATCCGCCACA70.1224632610216935No Hit
TTCCCAAACAACTCGACTCTTCGAAAGCGTCTTACATAGAATTGGTCACC70.1224632610216935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG70.1224632610216935No Hit
GGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCT70.1224632610216935No Hit
TGCGACCGCCGAGCCGCGTCAGGTGCTGAGCATCCGCCGCTCGACGATCG70.1224632610216935No Hit
TGTTGACCTTGCGACCGCCGAGCCGCGTCAGGTGCTGAGCATCCGCCGCT70.1224632610216935No Hit
CTGCAGACCCTGCAGAACCAGGTGAACACCAGCGTCACCCAGTTCGTTGG70.1224632610216935No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG70.1224632610216935No Hit
GTACATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCC70.1224632610216935No Hit
CCAAGGGACTTGTCCATAGAGCCGCACGGAAAACACGTCTCGAGATTACA60.10496850944716585No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG60.10496850944716585No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG60.10496850944716585No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT60.10496850944716585No Hit
GAGCATCCGCCGCTCGACGATCGGCTATGTCAGCCAGTTCCTGCGCGCGG60.10496850944716585No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA60.10496850944716585No Hit
TCCGAGATGAATCCGCGTGCAATGTTGACGCGCTGCTGCTCGCCGCCGGA60.10496850944716585No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA60.10496850944716585No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC60.10496850944716585No Hit
GCCATAATCATAATCATAATCATAATCATAATCACAACAGCAGCGGCCTG60.10496850944716585No Hit
GGCTATGTCAGCCAGTTCCTGCGCGCGGTGCCGCGGGTGGCGACCATCGA60.10496850944716585No Hit
ACCATCGACGTCGTCGCCGAGCCGTTGATCGTCAACGGCGTGGCGCGCGA60.10496850944716585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT60.10496850944716585No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG60.10496850944716585No Hit
ACCTTAGTAACGGCGAGTGAAGCGGTAAGAGCTCAAATTTGAAATCTGGT60.10496850944716585No Hit
GCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAA60.10496850944716585No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA60.10496850944716585No Hit
TTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATT60.10496850944716585No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA60.10496850944716585No Hit
GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT60.10496850944716585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.10496850944716585No Hit
GATATACCATGGGCAGCAGCCATAATCATAATCATAATCATAATCATAAT60.10496850944716585No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT60.10496850944716585No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG60.10496850944716585No Hit
GCCGCATCGAGCGAAGCGGTCGGCTCGTCCAGCAGCAGGATCGGCAGGTC60.10496850944716585No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT60.10496850944716585No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA60.10496850944716585No Hit
GTACATGGGTCTGGTCTCAAATCAGGTAGGATCACCCGCTGAACTTAAGC60.10496850944716585No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT60.10496850944716585No Hit
GTGCAATGTTGACGCGCTGCTGCTCGCCGCCGGAGAAGGTCGACGGCGGC60.10496850944716585No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA60.10496850944716585No Hit
GAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGCGAAGCGGG60.10496850944716585No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG60.10496850944716585No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA60.10496850944716585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)