FastQCFastQC Report
Tue 10 May 2022
EGAF00003672171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17295
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA370.21393466319745588No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA360.20815264527320035No Hit
TCTATGATCTGTCCCTGCTCCAATTTGTAAAAAACGCAAGATTCATCAGA330.19080659150043364No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA330.19080659150043364No Hit
ACCTACACGTGTCCGTGGAGTCCCGTGGCCAGCCTGTCAAGATCTATGCC280.16189650187915583No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA260.1503324660306447No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA250.14455044810638912No Hit
GCTATGCATTGCATGGCATCCGCTACAAGAACGACCATATCAACCTGGCC250.14455044810638912No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG240.13876843018213356No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC230.132986412257878No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA230.132986412257878No Hit
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA210.12142237640936687No Hit
GTATAAAATCAGGCAGTATTTTGATCACGTTTATTGTAAGCCGTCAGCAT200.11564035848511131No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT200.11564035848511131No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA190.10985834056085574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG190.10985834056085574No Hit
GTACATGGGAGGTGTTTTTCCAGGTCTGACCACCTGGCCCTGCACATGAA190.10985834056085574No Hit
GTACATGGGAACAGCTATGCATTGCATGGCATCCGCTACAAGAACGACCA190.10985834056085574No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG190.10985834056085574No Hit
GTACATGGGGGATCCACATCCGCAGCGAGACCTCCGTGCCTGACCATGTC190.10985834056085574No Hit
GTATCAACGCAGAGTACATGGGAAGGGATCAACCTTTCCCGGCAAGGCGG190.10985834056085574No Hit
ATATCAACCTGGCCGTGCTGGCGGATGCCGAGGGCAAGCCCTACCTACAC190.10985834056085574No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT180.10407632263660017No Hit
GCATTGAACATTCGTTCTGTCAGCATCCGCTCCAGCTTCACTGCATCAGC180.10407632263660017No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG180.10407632263660017No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT180.10407632263660017No Hit
GTACATGGGAACCTGGGAAGGAATTCAGGCTGGAAAGGAGCTCGAGGAGC180.10407632263660017No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG180.10407632263660017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)