FastQCFastQC Report
Tue 10 May 2022
EGAF00003672221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28038
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA3671.308937870033526No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT3511.2518724588059063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.7739496397745916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.5064555246451244No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1070.38162493758470645No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.31385976175190816No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT860.30672658534845565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.2995934089450032No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC800.28532705613809833No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC800.28532705613809833No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT770.27462729153291965No Hit
GTACATGGGAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC750.26749411512946714No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG710.2532277623225622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.2389614095156573No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA640.22826164491047862Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG640.22826164491047862No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC630.22469505670875242No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC580.20686211570012125No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.19616235109494257No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT550.19616235109494257No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT510.18189599828803768No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA510.18189599828803768No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG490.1747628218845852No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT470.16762964548113277No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT460.1640630572794065No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGG440.15692988087595408No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC440.15692988087595408No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT440.15692988087595408No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.15336329267422782No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCG420.1497967044725016No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT420.1497967044725016No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG420.1497967044725016No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC410.14623011627077537No Hit
GTACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT410.14623011627077537No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG410.14623011627077537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT410.14623011627077537Clontech Universal Primer Mix Long (96% over 26bp)
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG410.14623011627077537No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT390.1390969398673229No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT390.1390969398673229No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC380.13553035166559668No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA380.13553035166559668No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA370.13196376346387045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG370.13196376346387045No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC370.13196376346387045No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA350.124830587060418No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG350.124830587060418No Hit
GTACATGGGAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC340.12126399885869178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.11769741065696555No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT320.11413082245523931No Hit
GTACATGGGAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACC320.11413082245523931No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA320.11413082245523931No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG320.11413082245523931No Hit
CCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT310.11056423425351308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.11056423425351308No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG310.11056423425351308No Hit
GTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTCAGCCCCTC300.10699764605178687No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT290.10343105785006063No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG290.10343105785006063No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG290.10343105785006063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)