FastQCFastQC Report
Tue 10 May 2022
EGAF00003672264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44989
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA6081.3514414634688479No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT5281.1736202182755786No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG910.20227166640734404No Hit
GTACATGGGAACCCCCACCGGACCTACAACCCGCAGTCCCACATCATCTC830.18448954188801706No Hit
GTACATGGGTGGCCGTCAGCATCCCGATCATCAAGGCGCTGCGCGAAGGC830.18448954188801706No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC750.16670741736869013No Hit
GTACATGGGCATCGGCACTGCCATCGGGCAGATATTCTTTCAGGATAACT690.1533708239791949No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT690.1533708239791949No Hit
GTACATGGGCGGGCACGGAGAACATCTACAAGATCTACGGCGAGAGCTTC680.15114805841427906No Hit
CTCATAGACAGACCAAGAAATGGCGGTGGAGGGCATCTGGTAGATGACAC660.14670252728444733No Hit
GTACATGGAACCGCCGTCGCGCCATCCGCGATTTCGACAGAACCGCGGTC590.13114316833003622No Hit
GTTTTCAGTCACTGCAAATTTTCTTCCCTCCTCTGGGATCTAAGGATCCA590.13114316833003622No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG590.13114316833003622No Hit
GTCTATGAGTTCTTCAAGTACTTTCTCACCAAGCGCCAGCTGGAAAATCG580.12892040276512035No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA580.12892040276512035No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT580.12892040276512035No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG560.12447487163528863No Hit
GTACATGGGGGATCGCGGTGCTGCTGATGTCGGGCTTCTCGTCGGAACTG530.11780657494054102No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT520.11558380937562514No Hit
GTACATGGGTCCCTGCTCACTCTACTGTCGGAACTTGGTGTCGAACGGGC520.11558380937562514No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT460.10224721598612996No Hit
GTACTACGGCGCGGGCGCCGGCTCGGAACAGACCGCTTCTGCCGTGATCG450.10002445042121408No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA450.10002445042121408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)