FastQCFastQC Report
Tue 10 May 2022
EGAF00003672321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338113
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC25110.7426511255112935No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT20220.5980249206626187No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13860.40992212662630545No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA13010.38478260226610633No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT11400.337165385536788No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11280.3336162762153481No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA11190.33095444422426823No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA10730.3173495251587487No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10690.31616648871826875No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10640.3146876931676688No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT10450.30906827007538895No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10090.2984209421110694No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA9860.2916184825783096No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT9260.27387293597111023No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC8930.2641128853371506No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8670.25642314847403086No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT8670.25642314847403086No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG8340.2466630978400712No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT7430.21974901881915218No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT7400.2188617414887922No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG7360.21767870504831224No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT7080.20939744996495255No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT6930.20496106331315272No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT6870.20318650865243276No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG6720.1987501220006329No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC6660.196975567339913No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG6640.196384049119673No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6520.19283493979823313No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT6310.18662399848571334No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA6290.18603248026547337No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG6140.18159609361367354No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC6050.17893426162259363No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT5950.17597667052139374No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT5880.17390635675055383No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG5760.17035724742911393No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA5690.168286933658274No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG5500.1626675105659942No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA5450.16118871501539428No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA5380.15911840124455434No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC5310.1570480874737144No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC5260.15556929192311444No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG5050.1493583506105947No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA5040.14906259150047468No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC5020.14847107328023473No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG4960.14669651861951477No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC4940.1461050003992748No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT4850.1434431684081949No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG4840.1431474092980749No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT4800.14196437285759494No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG4730.139894059086755No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG4700.13900678175639505No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA4670.13811950442603507No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG4660.1378237453159151No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG4650.1375279862057951No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA4540.13427463599447523No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG4540.13427463599447523No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC4480.1325000813337553No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA4470.1322043222236353No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG4310.12747217646171546No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA4250.12569762180099553No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA4180.12362730803015559No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG4180.12362730803015559No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA4160.12303578980991563No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC4140.12244427158967565No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT4040.11948668048847576No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC4030.11919092137835575No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC3940.11652908938727587No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA3940.11652908938727587No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC3890.1150502938366759No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA3820.11297998006583598No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG3790.112092702735476No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT3780.11179694362535601No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT3760.11120542540511605No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT3760.11120542540511605No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA3760.11120542540511605No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA3760.11120542540511605No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA3750.11090966629499605No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC3740.11061390718487606No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA3670.10854359341403615No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA3620.10706479786343617No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG3600.10647327964319621No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT3590.10617752053307622No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG3580.10588176142295623No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC3570.10558600231283623No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA3570.10558600231283623No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC3560.10529024320271627No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG3560.10529024320271627No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT3560.10529024320271627No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA3530.10440296587235628No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC3520.10410720676223628No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG3510.10381144765211629No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT3500.10351568854199632No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT3450.10203689299139637No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG3400.10055809744079641No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT3400.10055809744079641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)