FastQCFastQC Report
Tue 10 May 2022
EGAF00003672435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27538
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA9233.3517321519355074No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT7082.570992809935362No Hit
AACATATACAGCATTCATTCGCTGAGTACCTGCCAAGAGCGAGGCTAGGT510.18519863461398794No Hit
GTACATGGGAGTGGCTGCATTCGGCGATACCGCTGGCCGATTCCACCACG510.18519863461398794No Hit
GTACTATGTTAGCCCCATAATTTTTTTTTTCCTTTTAAAACACTTCCATA480.17430459728375336No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG480.17430459728375336No Hit
GTGTTAGGATTAGGTATATATTTTAAAAGGCATGGCTGGGTGCGGTGGCT440.1597792141767739No Hit
GTCCAGGCACTGCTGCCCAGCCTCCAAGCTCCATCTCCACTCCAGATTTT420.1525165226232842No Hit
TCCATAATCTGGACTCCTCTGTCCAGGCACTGCTGCCCAGCCTCCAAGCT410.14888517684653935No Hit
GTACATGGGACTCTCCCTACCCATTCCTGCCAGCTCTGCCTCCTTTTCAA400.14525383106979448No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT400.14525383106979448No Hit
GTATACGGGCTCGAATCGCCACCGTGGGCCCTGTTCCTGCCGTGCCCCTG390.1416224852930496No Hit
CTCCCTACCCATTCCTGCCAGCTCTGCCTCCTTTTCAACTCTCCACATTT350.12709710218607015No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT330.11983441063258043No Hit
CATATACAGCATTCATTCGCTGAGTACCTGCCAAGAGCGAGGCTAGGTGC310.11257171907909072No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT310.11257171907909072No Hit
GTACATGGGTGTTTGTATATGCTAAGATCGCCATCATCAAAGGTTATGAG310.11257171907909072No Hit
GTACATGGGAAGATGGACACTGGGCCACCCTCCCAGGCACCAGACACAGG310.11257171907909072No Hit
GTACATGGGTCGACGCCGATCCGTACCTGGTCCACCACGAAGTGGACGAT300.10894037330234584No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA300.10894037330234584No Hit
GGATACGCCTGCTGACGCAAACAAGAGAGGCGATCAGCCAATCGGCGTGA290.10530902752560098No Hit
GATCTATACTACCCAAAGTGATCTACAGATTTAATGCAATTCATGTGAAA280.10167768174885612No Hit
GTACATGGGAGGTCGGAGTAGGCGTCGTTGTGGATCAGGTGGCTGGGCGT280.10167768174885612No Hit
GACATAGACATAGGCCTAGACACCGACACAGACACCGACACAGACACAGC280.10167768174885612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)