FastQCFastQC Report
Tue 10 May 2022
EGAF00003672436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27538
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA8393.046699106688939No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT7772.8215556685307575No Hit
GTGTTAGGATTAGGTATATATTTTAAAAGGCATGGCTGGGTGCGGTGGCT610.22151209238143654No Hit
GTACATGGGAGTGGCTGCATTCGGCGATACCGCTGGCCGATTCCACCACG520.18882998039073282No Hit
GTACTATGTTAGCCCCATAATTTTTTTTTTCCTTTTAAAACACTTCCATA470.1706732515070085No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG440.1597792141767739No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG440.1597792141767739No Hit
AACATATACAGCATTCATTCGCTGAGTACCTGCCAAGAGCGAGGCTAGGT410.14888517684653935No Hit
GTCCAGGCACTGCTGCCCAGCCTCCAAGCTCCATCTCCACTCCAGATTTT360.13072844796281502No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA350.12709710218607015No Hit
TCATAAAGAAGAGAAGAGAGACAAGATTAAGCATGAGACCATGAGGCTGC340.12346575640932529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.12346575640932529No Hit
GTACATGGGTCGACGCCGATCCGTACCTGGTCCACCACGAAGTGGACGAT330.11983441063258043No Hit
ATGTTAGCCCCATAATTTTTTTTTTCCTTTTAAAACACTTCCATAATCTG330.11983441063258043No Hit
GTACATGGGAGGTCGGAGTAGGCGTCGTTGTGGATCAGGTGGCTGGGCGT330.11983441063258043No Hit
GTACATGGGTTCGGCCTGGGGAGCAGCCGACCCGGTACCCGGTCTGCTGG320.11620306485583558No Hit
GTACATGGGACTCTCCCTACCCATTCCTGCCAGCTCTGCCTCCTTTTCAA320.11620306485583558No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT320.11620306485583558No Hit
GACATAGACATAGGCCTAGACACCGACACAGACACCGACACAGACACAGC310.11257171907909072No Hit
TCCATAATCTGGACTCCTCTGTCCAGGCACTGCTGCCCAGCCTCCAAGCT310.11257171907909072No Hit
CTCCCTACCCATTCCTGCCAGCTCTGCCTCCTTTTCAACTCTCCACATTT300.10894037330234584No Hit
GTACATGGGAGTGCGGGTTGCGCCAGCAGCCTTGGCGGCGGCGGCGCCTC300.10894037330234584No Hit
TTGTAAAACAGAGCTGAAGTCACAGGAAGTAGGGAACTTTGCACCCAACA300.10894037330234584No Hit
CATATACAGCATTCATTCGCTGAGTACCTGCCAAGAGCGAGGCTAGGTGC290.10530902752560098No Hit
GATCTATACTACCCAAAGTGATCTACAGATTTAATGCAATTCATGTGAAA290.10530902752560098No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT280.10167768174885612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)