FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003672525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGCCCTCATCCTGCACCACGACGGCGTGCTGCGCCAGCCGCTCCT38.333333333333332No Hit
CAGGATGCAGATTGGTGGCTTTGGTTTAACACTGGGCACACGAGAAGCCA25.555555555555555No Hit
GATGACCCGTCGGATCTCCGACCTCACCGAGCACCTCAAGGAGCACAAGC25.555555555555555No Hit
CCTGTCGAGCGCGGACTTCGACCTGCTGCACGCCTTGATCCGAAACGGCG25.555555555555555No Hit
TCGCCAAACCCTGGGCCTGGCGAGCGACACCGCGTCCAATCTCGTAACCG25.555555555555555No Hit
CTCCGGCACCGCGGCCGAGGACGACAGCACGGTGACCGACTCGCACGCGA12.7777777777777777No Hit
ACCATGCAAAGTTTGGCCTCTTCTGGGCTCAGACGAACACCAACAGCTGC12.7777777777777777No Hit
GTGCTCTGGTGGAGGTAGCAGGGCAGTGAATTGAACTCTTTGAGGGTCCT12.7777777777777777No Hit
CCCTAGCACTCCTTCTTGGCTGGACACGGCTCGAACGGCGCATTCCCAAA12.7777777777777777No Hit
CCTGCGGACACTCCGCGTCCTCGCGCGCTCCTGCGGAGGAACCCGACGAC12.7777777777777777No Hit
GTTCACACTCCAGCGGGTCGGACAGCGGCCGACGGTTGGAAATAAGTCAA12.7777777777777777No Hit
GGTAAAGCGATGGAGCGTACGTATACCCCTTTACTCGCTACCGTCAGATT12.7777777777777777No Hit
GTGTCGGAAACGACGGGGACCGACACCTCGCGCATGCGGTCCGACGTGCT12.7777777777777777No Hit
GGCGGCGCCCACGCGGGGATGCGCCGCGCCCAGCGTCTGGAGGGCGGCCT12.7777777777777777No Hit
GCCCTTTTCACAATCGACCAAGGTTCAGCCGAAAGGCTTACGCCATAACG12.7777777777777777No Hit
TGCATGAATCGGGCGCGATCCGTCCGGGAATCGAGAGGGACACCGTGAGC12.7777777777777777No Hit
CATCGCGCGTCGCGACCAGCCCGGGTGCCACCTACCCCTCCTCGACCACG12.7777777777777777No Hit
GTTGGTTGTAATGATGACAATAATGATCAAACCAACTCCAAACAACCGAA12.7777777777777777No Hit
CTCCTGAGTAGTGGGTGGGACGTCTGCCTGGGGCAGAATTAGTCTCCCTC12.7777777777777777No Hit
CCACGACGCCGAAGCCGCTGACAGCCAGCCCGTAGCTCACCACTGCCCAG12.7777777777777777No Hit
GGACGACCTGTGCATCACTGACGGTGAGCCTGAGTGGCTATCCAAGGATT12.7777777777777777No Hit
GATGAGGATGTTGAGCAGTCAATCGAGAACGTCCAGCAGGACTTCGACGT12.7777777777777777No Hit
GACAACAACTCTTGTGCCCCGTCGCCGGTTGCAAAAAAGTGGTGAGACAA12.7777777777777777No Hit
GTTCACACTCCAGCGGGTCGGACAGCGGCCGACGGTTGGAAAAAAGTCAA12.7777777777777777No Hit
TCCTGCTTCCTGTGCAGTGAGTACGCCCCAGGCCCTATTGCTGTTGCCTT12.7777777777777777No Hit
GCCAAGCATTCGCCGACGCAGTCGCGGGTGACTATGGCGGTGCACCTTTC12.7777777777777777No Hit
CCGGAGAGGGACCGGATCTTCGTCATCCTCAGGCCCAGCTCCTCGACGAT12.7777777777777777No Hit
CCGCCGGTTTGGGCGACGCGCTTTGATTGTCGCCGCCACCCGCACCAGCT12.7777777777777777No Hit
GTCGTTCGCCCCGTGGTTGCCGAAACTACTGCTCTGGGCGCTGCCTACGC12.7777777777777777No Hit
GGTGAGAACGCCATCGTACAATGTCCCCGGCGACCTGGATGTCATCCCGG12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)