FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003672526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGACGGTGGGAAAGCTCAAGCCGGTGTGAGCGGCGAGCGTGTCGATGT38.333333333333332No Hit
TCCCGGCTGACGAGGAAGACCGGCAGGACGAGGAACTCCGAGATGAGCAG25.555555555555555No Hit
GAACCATCAGACCGCGGCGGGTGTGGTGGTCCTGCTTGTGCTCCTTGAGG25.555555555555555No Hit
GCCTTGACATGGCTTCTCGTGTGCCCAGTGTTAAACCAAAGCCACCAATC25.555555555555555No Hit
GAAGTCATAGTTCTCAAGCTGTTCCTGCAGAAGGACAAACCACAGGATGG12.7777777777777777No Hit
ATCTTTTCATCGGCGAAACGGGCCGTCACCAGGCTTTCGGTGGCGCCGAG12.7777777777777777No Hit
CTTCAATACTTACGCCGTCAATTACTTTAGTTTTTTTACGATTAGCAAAT12.7777777777777777No Hit
ATCCACAATCGTCCACTCGCAATCTGCAGGGTATGATACTGTTTCAATGT12.7777777777777777No Hit
GCTCATCCTGTGCCTGACCATGACGGCGACGATGCGCAGTGCCAGAGGCT12.7777777777777777No Hit
GACCAGAACGGGGGGTTGTGGTGCTCGCCGATGGCGAAGACGTCGAGGCC12.7777777777777777No Hit
ATGTAGACTAGCACGATCTCTCTTCTGCCCACGCATCTTTGCCAGTGCCG12.7777777777777777No Hit
CCTCCACGGAGACTATCCATGAATCTACGCGGGAGACCGGACCATCGCGC12.7777777777777777No Hit
GTGAAGCGCCCCATGTGGTCGCGCCGCGCCCAGGAATATGAGGCGAAGAT12.7777777777777777No Hit
GTCGGGTTCCTGGCGTCATCGCCCAGCTTCTGACGCAGACGGCTCACCCG12.7777777777777777No Hit
GCTCGTGCGCACCCGGCGTCAGGGGCGGCTGCTCGCCGAGCTCGAGAGCG12.7777777777777777No Hit
CCCCGGGCAGTTCTGCCGGCAATCGGTGCTCTGATGATCGGCGGCGGGGA12.7777777777777777No Hit
ATGCTGATCAGATCTGGTACTCGGGCGGGAGAACTCCGCAACGCACAATA12.7777777777777777No Hit
GTTGCGCGGCCGCGCGGTCACCGTCACCGGTGAGCGCGGTCTGGACCTGG12.7777777777777777No Hit
TCACAGTGCTCAATGGAAGTCCAGGTTACATCAACCTGCTCGACTCGCTC12.7777777777777777No Hit
ACGTGATTGTTGGTGACGACGTGGCCGGCCGCGTCCCAGACGAAGCCGGT12.7777777777777777No Hit
CATCCCCGCCCGGAAGTATCCGGGGTGGATCAGGCAGGGCCTGACCTGGG12.7777777777777777No Hit
GAGTGGAAAGTTTTCTTAACCAAGTCTTTACAGCTTGGCTTTCAGCCCCT12.7777777777777777No Hit
TCCCCGAGGGGGCCGACGGCGACAAGGCCCGGGACCGCATCCAGCCCGCG12.7777777777777777No Hit
TCGCCGGCCTCTCCATCGTCTTCGAGGGCCAGTACGCGGTCGGGGACTTT12.7777777777777777No Hit
GGCAATACCCAGGGACGCGACCAGATGAAGCCGGAAGTGGTCAACACTCC12.7777777777777777No Hit
GGCAATACCCAGGGACGCGACCAGATGAAGCCGGAAGTGGTCAACAATCC12.7777777777777777No Hit
GTCAAAAGTCTTGGTGACGGCCTTCTTCCAGTTGCGGAACAGACGCTCGC12.7777777777777777No Hit
GTCGGGTTCCTGGCGTCATCGCACAGCTTCTGACGCAGACGGCTCACCCG12.7777777777777777No Hit
ACTCAAGCCATTCAGCAACTCACAAAAGAACAACCCCACTTGAAGAAAGA12.7777777777777777No Hit
GGGTTAACCTCGCGGTCGGCCGGACGGTGAGGAGCCAGAACCAGGGCGAG12.7777777777777777No Hit
GACGTTTTGCGGCCTTGCCGCAAAACGAGTCGGCATGAGATATAATGGAG12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)