FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003672715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAGCCGATCGCGGAGCCGACCTTTTCGCCGATGAACAGCATGGGG47.6923076923076925No Hit
CTACCTTAGCGGGATTCACTGACCAAATAAAATATTCATACCGATTCTGG23.8461538461538463No Hit
GTCCGGGTCCATGTTCACAAAGCCGACCTCGAATCGGACCTGATCGTTGC23.8461538461538463No Hit
TCGTCGATAAGCGGATAGGCACAAAGACATTGACCACGTGCGTGCAGGAC23.8461538461538463No Hit
GTCGTGCTCGACGCCGACCCCGGGCCGGGCGGGGCCTGGCAGCACCGGTG23.8461538461538463No Hit
CACCAGCGCCATGCCGAAGGGGCTAGCGAGGTCGAGGCCCTGACCGTAGC23.8461538461538463No Hit
GATGTAACTGCCGCCGAAGGCATCCGGCGCGGTTGCCGCCCACAGTTCGG23.8461538461538463No Hit
GAACAGGATCATGACAGTGTCTTCTCCCAGGGGAAAAAAAATTAAAATAG23.8461538461538463No Hit
TGTAGGTCTTGAGTCCAATCCACAAAATCCTGCATACTAGACAAGTTTCT11.9230769230769231No Hit
GGGCTGATGGCTAGGCATCGCCTGCATGACGATCTGATCGAGCGTCTCCT11.9230769230769231No Hit
TTTGCGCTGAGAGCGATTGCCCAGCGGAAGAAATGGGCGAAGAATGCCGT11.9230769230769231No Hit
CCAATATTGTACCTGCTCTGGATTTATGGGATAACATTAGAAGCATGCTT11.9230769230769231No Hit
GTCCCGCAGCGTGTACACGGTCAGCCCGTTGGCGAGCCGCCGCGTCTTGA11.9230769230769231No Hit
GGAAATGGATCTCGGCCGCGCCGTCGCAAGCGTCATGGGCCTGGAATTCG11.9230769230769231No Hit
CACTCCGACGAAGAAACTGGGGCAGAATTTCGTCCATGATCCCAACACGG11.9230769230769231No Hit
CACGATTGGACGCGGGTCGATTCGGCCATCTTTCACGAGTTCCATACCCG11.9230769230769231No Hit
GCCCGACACAAGAACTTCCACGTCCACTTCACCCCCTGTCTCTTATACAC11.9230769230769231No Hit
CTCGTGAACCGACCGGAACGCACTCGATCGCGCGCACGAAGCCCGGAAGG11.9230769230769231No Hit
CATCAACGCCGCGCGCGAGCCGGAGATCGTCGACTTGGCGCGGCTGCTGG11.9230769230769231No Hit
CCATCAGATCAGTACGTGAGACGTCGGTTGCGTAGGCGTGTTGCCCCCAA11.9230769230769231No Hit
GCTCCATCTGGCAGGTCTCCCCCGTGATCGACCCCGCCACCCGGCAGGGC11.9230769230769231No Hit
GTACTCGATCGTGCACGACCCAGACAGCCCCGATGCCACAAGCACACCGC11.9230769230769231No Hit
CATAAATAGTCAGAAATTCAGCATCATTACCTGCTGTTTTAAATAAAAAT11.9230769230769231No Hit
TACTTATGCAATGTCATTCACATACCAAAGGGAATCCTACTTGCAATGAA11.9230769230769231No Hit
GGAAATTTGCAATCATGGCAGAAGGCAAAGAGGAAGCAGGCACATCTTAC11.9230769230769231No Hit
GGTCCATCATCATCGCCGACGTGTGGAAGACGGCCACCTACATCGGCCTG11.9230769230769231No Hit
GCATTTGTGTATTTAACTTCAGAGATTCTACACTTTTAGTTTTTACCCTT11.9230769230769231No Hit
GGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAACGTCAATG11.9230769230769231No Hit
GTTGGGGAACAGGGCGACGCACGGAATGCCGAGCGCCGCGGCCGCCTTCG11.9230769230769231No Hit
GTCAGGTTGCGTGATGAATACGTTCGTGCCATTCTTCGCGGACACAATCC11.9230769230769231No Hit
GTCAAGGAGGAGTTCCGGCTCCGCACCCAGACACCGCCCTACGGTCGGCT11.9230769230769231No Hit
GACTTGGAGGGCTCAGAAGACAGGAAAATGTGGCGAAGTTTGGAATTTCC11.9230769230769231No Hit
TGATAATTGCGCTCATCACTGCCGGATCCGGTATGCGGTGCATGTATGCA11.9230769230769231No Hit
GCATATTTGCCACAAGACTTCATCAACAATGCAGTGCTTTTCACTCTGGA11.9230769230769231No Hit
CACCTATGCCATGTTCGCCTGGTTGCCGGTGATCCTCAGCGAAGCCGGGC11.9230769230769231No Hit
CAGGATGACGATCCTGACGGGCGGCAATAGGCTAGCGACGGCAAGAGACA11.9230769230769231No Hit
CCGTTAGACACGGCGTTTACAAAACCCGCCCACATGTTATTGGCGGCAGA11.9230769230769231No Hit
CCGAAGTGGCCGACACCTTCGTCGAGATGACGGCGGCGCCCTGGTTCGCG11.9230769230769231No Hit
GGGAATAGGACAAGCTGAAGATTACAGGCTGAAGGTGCTGTCTCTTATAC11.9230769230769231No Hit
GGTGTGACACTCTCCTTCCGTTCTGGCCGCACGGGAGCGCGCGGGCGGTG11.9230769230769231No Hit
CTTTGGTGAAGTAGAAAAGTGTTCAGACAGCTGCAGTGAGTTTTTGTGTT11.9230769230769231No Hit
GTTATGAGCCCAGCGAGCTACCGAGCTGCTCCACCCCGCGTCGGGCGACT11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)