FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003672805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAAGAACCCGAGGAAAGCGGGGAGCTCGATGTTGTGATAGCGGTTG23.0303030303030303No Hit
GATCAACGAGAAGTTGTACACCACCGTTGCTGACGGCATGCGTACCCTCG23.0303030303030303No Hit
CCTCGGCCCAACCTCGCGGCAAACGCGTTCTTGGTCGTGAGCCAGGGCCT23.0303030303030303No Hit
GATGCGCACCATGTCCTTGATGCCCTGGCGCAGCAGCTTCGGCGGCAGCC23.0303030303030303No Hit
CACTGCAGCGGTCCACCTCCGGCGTGGATGCCATGCTCATCGGTAATGGC23.0303030303030303No Hit
GAGCGTGGCGGCGCCGCGCGTCACCGTGGTCGACACCATCGGCGCGGGCG23.0303030303030303No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCGGTTGCCCAGCCACTGCTGGTCGCCGCCGCGCTGGCCACAGCCT23.0303030303030303No Hit
CCTTCGCGTCTTACGCGCTCGCGCGCCTAGATTTCCGCGGCAAGGGCGGA23.0303030303030303No Hit
ACCACATTCCGGACAAGCCGCGCATCTACGCCTCTAAGGTGAAAAACGCT11.5151515151515151No Hit
CACCCTCACTGGGCAAGCTACCATCGCGAAGACTCGGGCAGAGGTCCTTA11.5151515151515151No Hit
ACATCGTCATCGCCACGAAAGTTGCTACCCATCCAGAGTTCAAGGGCTTG11.5151515151515151No Hit
ACGGCGGTGACGGGCTGTTCGCAGCTGCGGCTTTGGCCCAGCGCGGGTGG11.5151515151515151No Hit
CAATCACCGTGATGGGTGTTTCGCCAGCCATGTCAGTCTCCAGATCGCAG11.5151515151515151No Hit
GGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCT11.5151515151515151No Hit
GGATCAGGCTGTGGGCTCGCTCGGAGCCTTCTGGACGGACGGACACGAAA11.5151515151515151No Hit
CTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCC11.5151515151515151No Hit
TCATTGGCTCGACCGATCGGTATCACGTCCACTTTGTTGCCAGCCGCGAG11.5151515151515151No Hit
GATCCTTCACATCCCTTGTAAGTTGGATTCCTAGGTATTTTATTCTCTTT11.5151515151515151No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACAATGCTATGATACCACTC11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTGGCCTGCCCCTGCAGGCTTCAGAGACCACTGCGTTTCTTAGAAGG11.5151515151515151No Hit
AGCGTATTCCGTGTCAGGGTATGCCATTCTTCCGAGAACAGGACGATACC11.5151515151515151No Hit
TTGATTTTGTATCCTGAGACTTTGCTGAAGTTGCTTATCAGGTTAAGGAG11.5151515151515151No Hit
TGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACA11.5151515151515151No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT11.5151515151515151No Hit
CAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC11.5151515151515151No Hit
GACTTGGTGGATTTAGCCCGACCCGATCCGTGGCAGACCTGACAGGGGTC11.5151515151515151No Hit
CAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAA11.5151515151515151No Hit
TTTCTAGGAGGTGGAAGAATTGAGGGGAGGAGGACATTTTGCAAAGTCCA11.5151515151515151No Hit
GAGGAGCGCGTCGACCGGCTCGCCCTCACCGCCGAGGCCCTCGCCCTCCT11.5151515151515151No Hit
GTGAGATGTCATCTCAAAAAACAAACAAACAAAAAAGATATACTTCTACA11.5151515151515151No Hit
AGCATCACCCTTACATTAAAACCTGGCAGAGACACAACAAAGAAAATTTC11.5151515151515151No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAATTGGTA11.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCGATAGCTGACCAGTCATTGCGGCTAGCAGTACGTACCTGGCGCAGGG11.5151515151515151No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG11.5151515151515151No Hit
TTTTGGAGACGTGACGAAGTGGATTGGGACAAGCGCGGACAGACCCTCTT11.5151515151515151No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC11.5151515151515151No Hit
ATTTTGGCCTTTTTCATTGAGCTTGTAAGCATTTTAGAAATCTTCTGTTA11.5151515151515151No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ATACAAGCCGTTTGGTTCGTGCGGGTCTACGCCGCTGGTCCTATATCCCA11.5151515151515151No Hit
CCGCGAGACTCTGAGTGCCACCTCTGCATGTCCGTGACCACCCAGGCCGG11.5151515151515151No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGT11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ATGCTGTATCAATATGAAAAGGAGTTTTGAAAACAATCCTACTACTCTAC11.5151515151515151No Hit
ATCCTAGGAAAGAAGTCCTACTGATATTGTCGATAGTCTCCAAAAGGTGA11.5151515151515151No Hit
CTCTAGAGCAGATCTAGAGGAGGCAGGGAAGAAACCCTCAGATTTCTCCG11.5151515151515151No Hit
AGGTATGGCAGGTTGCGCATTGAGAAGCACCCCACCCATGGGCTGGTATT11.5151515151515151No Hit
GTGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG11.5151515151515151No Hit
CGTGTCGTCGGCGCGGGTCAGACGACCCTCACCATCCCAATCCCAGCGGT11.5151515151515151No Hit
CACTGCGGTTGCACAGCCACTGCTGGTCGCCGCCGCGCTGGCCACAGCCT11.5151515151515151No Hit
TCATCACCATGACGGTCGAAGGCGCATTCCGGAGCGGTCATCTCACCCCT11.5151515151515151No Hit
CTTTTCAGGGAAGAATGCCTCCGGATCAGTCTGGGCACATAATGCCTTGT11.5151515151515151No Hit
CTACCGCCTCAACCACCCAGGCTCCAGCGATTCCCCCCACCTCAGCCTCC11.5151515151515151No Hit
GTCCTCGCCGAGCAGACCTTTATCCCAGATTCGCTTAAGACCCCACCACA11.5151515151515151No Hit
CTGCTACTTCAAGTACCAGGTCCTGGCGATCAGCACCAAGAACCCACTGC11.5151515151515151No Hit
CTCCAACTACAACGACACTCCGCTGCCGGTCACGCGATCCATCACAGAAA11.5151515151515151No Hit
GGCTGAGGCGGGTAGATCACCTGAGGTCAGAAGTTCAAGACCAGCCTGGC11.5151515151515151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)