FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003672806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCATGCACAGGTCCCATCGCACCGGATGGGCCACCTGCTGGACCAG34.545454545454546No Hit
ATCTTGAGCATGCCGTCGTAGTTCTTGAACATGTGCTCAACAACCGGACG23.0303030303030303No Hit
GAGGGCGACCGCGCGAAGTCGCGCTCCAGTTCGGGCTGGCGCTGGCGGGG23.0303030303030303No Hit
CATGGTGACGGCGACGAGGAACTCGTTCCACGCACCAATGAAGACGATGA23.0303030303030303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCCCAGGTGGTCTATCTTGATCATCGCGAGGCCGCAGCAGGACACACC23.0303030303030303No Hit
GGGACAACGTGCGCGATGCGCCCAGCCTCGACGACGAGGTGATCCGTCCG23.0303030303030303No Hit
CTACTGGTGTGGCTGTTGGGTCTGGCCGTCGGCATCGCAAACGCGTGCGC23.0303030303030303No Hit
GACATGTTCAAGACTCTGCAGAAGATCGGTAAGGCGTTCATGCTACCGAT23.0303030303030303No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG11.5151515151515151No Hit
GTGTGGACTAGGGCCGGGGATGCTGCGAGTGGCGCGGGGGCATTATCGTG11.5151515151515151No Hit
GGCGAGGCTCATAGCGGTGGCCCCGACGACCGTTTTGCCAGCGCCGCAGG11.5151515151515151No Hit
TCACCCTCCCGAGTAGCTGGGATTACAGGTGCCCACCACCACACCCGGCT11.5151515151515151No Hit
GCATCGTCTTGCCTGCCTTCAGCGTCACGGCGGCAAAGCCGGTGATGAAC11.5151515151515151No Hit
GCTCTAGAGACTGCGGAATACCTGGACGGCCTCCAATGCCGAGAACAGCG11.5151515151515151No Hit
CCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCA11.5151515151515151No Hit
ATGCAGGGGTTCCGCGTCGACCGCAAGGCAGGCTGGGACTGTCACGGTCT11.5151515151515151No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA11.5151515151515151No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCACACACCCCGAGGGCCTGGCAGGTGGTGTGGGCATCCCAGCCCCT11.5151515151515151No Hit
GGCTCCGCTTTGTATAATCCCCTATGTCCACGTAGTGGATTCCTGTTATT11.5151515151515151No Hit
CACCGAGACTTTAAAGGCACCTACCTTGATTTTATCAAGCCGAGCATCCT11.5151515151515151No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
TCACAGAGGACTTCTTTCTGGAAACACCGTATATCTATTTCTCCACTGGA11.5151515151515151No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG11.5151515151515151No Hit
CTGTAGTATGGTTCAAGTCAGAGGAAAGGAAGATCCCTGGAACTCCATCA11.5151515151515151No Hit
GTGAAGAAAGGCTTTGAAATGTTTCTAAAGCCCTTTCCAAAATAAACACA11.5151515151515151No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC11.5151515151515151No Hit
ATCTGCGGCACTAGCAGGTGGGGCATGACGGATGTGAACCACGACCCGGG11.5151515151515151No Hit
ATGGGAGACTGTCCGGGGTGAACGGCACACTGGAACCATTGATTGCAAAC11.5151515151515151No Hit
GTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAA11.5151515151515151No Hit
GTTTCCACCATGTTGGCCAGGCTGGTCTGGAACTCCTGGCCTCAAGTGAT11.5151515151515151No Hit
TTCTGCATCTATTGAGAGAATCATGTAGTTTTTGTCATTGGTTCTGTTTA11.5151515151515151No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11.5151515151515151No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATCGGAAGAAGACGTA11.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGGTGAAGTGATAACCACGGCCCTGCCACTTCTTGTCGGGACCGCAGAC11.5151515151515151No Hit
CCTACCTGGTTTGGGTTCCACGAGGTTTTCCACAGTTGCACGGCGGTGGC11.5151515151515151No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11.5151515151515151No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTT11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTTAGCTCAGGAGTTCAAGACCAGCCTGGGCAACATAGCAAGATCTC11.5151515151515151No Hit
CCATAGCCCTGCGGATCCTCAACGTCTGCGCGATGCAACAAGTTGTACTG11.5151515151515151No Hit
CCCTTGTTACCCCTGCACATAATCCGGCGAGGGTGACCGGATTGAACTCT11.5151515151515151No Hit
GAGCTACACTGAAGGTTCGGCTACCTCTACTGCATACTTTCTATGGTGGC11.5151515151515151No Hit
ATAAATATCTCTGTGAATAAATTAAGGGCTTAGGAAGGATATACAGACAC11.5151515151515151No Hit
GTCGGAGACCAGCGCCCAGACCGCCAAGGCCGTCATGGAGGCTGCCCACG11.5151515151515151No Hit
CCCTGCCAGGCATGTCGGGGGACTGGAGCTCGGGCTGGTACCGTTCCGCG11.5151515151515151No Hit
TTTCTGCGCGTCATCGGAAGCTCCTTCGTCGACGGACTCGACGTAAGCGC11.5151515151515151No Hit
NATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGTTTTTTGAT11.5151515151515151No Hit
CCATTGACGGTCAGGTTTCTACCCGCAAGTGGTGGGACCTAGAGGCTCGC11.5151515151515151No Hit
TTTTAATACATGGGGGTCTCACTATATTGCCCAGGCTGGTCTCAAACTCC11.5151515151515151No Hit
AATTGTCCCTGTTTGCAGACGACATGATTGTATATCTAGAAAACCGCATT11.5151515151515151No Hit
GGCCGACGGGGGCGTCGTCGTCGAGGAGGGCGAGGGCCTCGGCGGTGAGG11.5151515151515151No Hit
CCTCCACCATGCCCGGCTGATTTTTGTATTTTTAGTAGAGACAGGGTTTC11.5151515151515151No Hit
AACAAGAGCTATTTATGACAAACCCACAGCCAATATCATACTGAATGGAC11.5151515151515151No Hit
GCTCAGTAATACGGCCTACGCAGATCACTCTCTCACCGGAGTAAAGAATG11.5151515151515151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)