FastQCFastQC Report
Tue 10 May 2022
EGAF00003672837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396813
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG11650.2935891717257247No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG11330.2855249197984945No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT10070.25377192783502556No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA9760.2459596837805213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.23764342398056518No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC9250.23310728227149816No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG7380.18598181007174663No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT7110.17917759750814616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.17791755814451643No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT6840.17237338494454568No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCTACCCAGTATCGCGTATG6460.1627970857809598No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG6290.15851295194461876No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG6230.15700090470826308No Hit
CCTTTAACCTCTCCGCCCTTATCACAACACAAGAACACCTCTGATTACTC6230.15700090470826308No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG6180.15574086534463336No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG6030.1519607472537442No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA6020.15170873938101825No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC5950.14994468427193666No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC5930.14944066852648477No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA5890.148432637035581No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCTACCCAGTATCTCGTATG5730.1444005110719659No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC5370.1353282276538319No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG5130.12928003870840923No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT5020.12650795210842386No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT4980.1254999206175201No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC4930.12423988125389038No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG4900.12348385763571254No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT4840.12197181039935688No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT4780.12045976316300122No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC4740.11945173167209742No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA4740.11945173167209742No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT4570.11516759783575638No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA4480.11289952698122291No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT4350.10962342463578562No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT4230.10659933016307428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.10659933016307428No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG4220.10634732229034835No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC4140.1043312593085408No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG4120.10382724356308891No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT4090.10307121994491107No Hit
GTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTC4080.10281921207218514No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC4010.10105515696310352No Hit
ATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAA3970.10004712547219975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)