FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003672855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
CGCTCTGCGGGTTCCTCCCTTCTTCCGAGCCTCTCCTCTGGCCGCCGCAC33.4090909090909087No Hit
ATGCTGGTGTGTGTGGGGGGCAGAGGATTTCCACACAGAGGATCAATAAA22.272727272727273No Hit
GCATAAAGCTAAATAACTAACTATAGGTCCTCAATTTTTTACTGTACCTA22.272727272727273No Hit
GTACATTCCAGGGCAACAATGGAGGAAGGGCAGGCAACGCATGAGGAATT22.272727272727273No Hit
GAGCCGACATCGAAGGATCAAAAAAAAGCGACGTCGCTATGAAAGCGGGG22.272727272727273No Hit
ATGGCATCCTGGGCATGGCCTACCCCCGCATCTCCGTCAACAACGTGCTG22.272727272727273No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA22.272727272727273No Hit
GGCAATGCATTGAGACTTTGTCTACTAAAAATCAAAATAATTAGCTGGCT22.272727272727273No Hit
GCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCA22.272727272727273No Hit
TCTTCAGACACTAAGGACAGGAAATAAAATGGGTTAATGCTACTGGTTGA22.272727272727273No Hit
GTGCTGTACTGTTCCGTACGGCAGCCACTACCCACATGTAACTTTTTAAG11.1363636363636365No Hit
CCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGG11.1363636363636365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTCGGGTAAATTGTAATTTTTT11.1363636363636365No Hit
TGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365No Hit
ACACAGGGATGTGGCCCCTGCGGCACCACCCGATGCCAGATTCCCTGCAA11.1363636363636365No Hit
GCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CAACTTAAAGACACTAGCCACATTAGCCACTAACAGGAGAGATGGGGTTT11.1363636363636365No Hit
GAAAAAAAATAAAAGCAATGTACATGTTGCCAAGATAACCTGAAAGACCA11.1363636363636365No Hit
GTACCCTGTGGCCCATTTCATTGACCAAACTTTGAAAGCCAAGGTGGGGC11.1363636363636365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365No Hit
GTGTTGGGACTGCTGATAGGAAGATGTCTTCAGGAAATGCTAAAATTGGG11.1363636363636365No Hit
GATAAAACAACAACAACAAAAAAAAAAACTTCAAGTTTCAGGATACAAAA11.1363636363636365No Hit
ACCTCCACCAACATGATAAGAGACAGAAAGATGGCAAACGTGATCATAAG11.1363636363636365No Hit
GGCGCATCCGTTGTAGCAGCAGCGCCGGTGCCGCGGGCACTCGGAGTCCG11.1363636363636365No Hit
GTACTAGGCAGATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCC11.1363636363636365No Hit
ATCCAGACCTCCAACTCGACATCCGCGACGAAGTCGCCATCGTCCGCCTC11.1363636363636365No Hit
ATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGA11.1363636363636365No Hit
CTGCAACGGGTGCCAAATGTTCGCCGCGCTCGCCGACCTCATCCCGGGCG11.1363636363636365No Hit
CCTTCCAGCCCAGAAGCAAGTGTGGCAATTCTTCCCATCACAGTGCGATC11.1363636363636365No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGCGGCAAAAGCTCGCTTGATCTTG11.1363636363636365No Hit
GTTTAAAGGTATTCCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGT11.1363636363636365No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CAGGTGGCATCTGGTGTGCTGTTCTTGAGTTTTCGTTTAGGATTAGTTGA11.1363636363636365No Hit
GATCTCCTGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGA11.1363636363636365No Hit
CACAAAACCAATTACTAACAAGCACCCAAGAGTGTTCAAACTGAAACAAT11.1363636363636365No Hit
GGCCTCGAACGGGATGGCAGAGGAGGTGAGCACCCTGATGAAGGCCACGG11.1363636363636365No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCACCGTCACATCGCGTATG11.1363636363636365No Hit
CTGCTGGTAATGAATTCCTCAACATTTGTTTGTCTGAAAAGGATGTTATT11.1363636363636365No Hit
TTTGAAAACATGGCAGAACCAGGAAGGTCACTCTCATCATTCCCTCTTCT11.1363636363636365No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTCACCGTCACATCGCGTA11.1363636363636365No Hit
CTACTGTTGCATGGGCTCGGCCGATGGGTATTGAACAGGTGCCGCGCAAC11.1363636363636365No Hit
GCACTGAGGCTGAGTTCGAGGAGCCCAGGGTGATTGACCTGTGGGACCTG11.1363636363636365No Hit
TTGTGAAGCTGCATGTGGGCACTGAGCAGAGTGCTGCTGTCAAAGGCTTT11.1363636363636365No Hit
GTTGATACCACTGCTTCCCGTGTACTCTGCGTTGATACCACTGCTTCCTG11.1363636363636365Clontech SMART CDS Primer II A (96% over 26bp)
GCCTGGGAGACGGCCTCTGTGGTGCCCAGCAGCTTTGCGGCTTGGAACTG11.1363636363636365No Hit
GACAATAACAAACTCGATATCTTCAAGAAGATACGTTCCTTGTGGGTAAA11.1363636363636365No Hit
ATCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT11.1363636363636365Clontech SMART CDS Primer II A (96% over 25bp)
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGT11.1363636363636365No Hit
CTGCAAACTCTGCCTCCCAGCTTCAAGCAATTCTCCTGCCTCAGCCTACC11.1363636363636365No Hit
ATCCTGGAGGTATTAAATGTCTAGTTAAATGTGTTCCTTGGGATTCTTCT11.1363636363636365No Hit
CCATTAAGACAATCTATAACAAAAAAAAGTATATTAGCACACATGGTATA11.1363636363636365No Hit
ACTCTCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC11.1363636363636365Clontech SMART CDS Primer II A (95% over 24bp)
TCAGAGACGTGATCATTTTCCATCATAGGTCTGTCTCTCTTTGGTAAGAA11.1363636363636365No Hit
AGCAGCAGGACGCTGAGCAGAGGCAACCGGCGGGGTCTGGCCGGGGATCC11.1363636363636365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
TCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGC11.1363636363636365No Hit
CCCTTCACAAAATCAATGAATCCAGGAGCTGGTTTTTTGAAAAGATCAAC11.1363636363636365No Hit
ACAATGTGCAATCAAAGTCCTCGGCCACATTGTGAACTTTGGGGGATGCT11.1363636363636365No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA11.1363636363636365No Hit
AGGAAGGAGTGATGAAAACAAGGCACCAGTCCCCTTGACAGGACCAAATC11.1363636363636365No Hit
ATCTCAGCTCACTGCAACCACCAACCTCCCGGGTTCAAGTGATCCTCCTG11.1363636363636365No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAGCTTC11.1363636363636365No Hit
GGCCAAGACCATACCCGCCGAATGTAGGTGAGGAAATCCAAATTGGTCAC11.1363636363636365No Hit
TGACGCTCATGTGCATGCCCTAGGCGAAGGAGCTAGGAGTCGTGTCGGAG11.1363636363636365No Hit
CCTCCAGAGTGCTGGGGTTACAGGCATGAGCCACCACACTCAGCTGGTTG11.1363636363636365No Hit
TGACTTCCCTCAAAGGCGTTTTTAAAAATGCTTTGAAGATCTTGAACTGA11.1363636363636365No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA11.1363636363636365No Hit
TTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCAT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
ATATCTCAAAAGTTGTTTTTAAAAATCTTGGTAACATCACAATCTTACAT11.1363636363636365No Hit
CATCTGTCCACTGAGACAGCATGAGTGCAGTGACACCCCAATAGTAATGA11.1363636363636365No Hit
GCGACGGCGGGCGCGGCCGTCGTTCCCGGCGGCGAACCGGGCATGGCCAC11.1363636363636365No Hit
TTCCTTCAGTTCTTTGACTATCTCATCCTATTCTCTCCTGTCCTTTAAGG11.1363636363636365No Hit
GAGAAATGCAAACCAAAACCACAATGAGATACCATCTCACACCAGTTAGA11.1363636363636365No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)