FastQCFastQC Report
Tue 10 May 2022
EGAF00003672964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003672964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13952
Sequences flagged as poor quality0
Sequence length100
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362625.989105504587158No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2551.8276949541284404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG930.6665711009174312Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG900.6450688073394496Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG640.45871559633027525Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT580.415711009174312Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC510.3655389908256881No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA460.32970183486238536No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT420.30103211009174313No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT410.2938646788990826No Hit
GTTTATCATAGTTTGCAACTTGGCCTACACGAGTGTTCTCTACCTGATAA410.2938646788990826No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT400.286697247706422No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG390.27952981651376146No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT380.2723623853211009No Hit
TGCCGGGACGTCGCGAGTCTCGAAGGTCTCGCGAGCGATGACGATCGGGG380.2723623853211009No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT380.2723623853211009No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT370.2651949541284404Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA370.2651949541284404No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC370.2651949541284404No Hit
ATAATGGCGCAGGCCAAATCAATCTTCTTGGTCATACCCGCGGAGTAGTC330.23652522935779818No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC320.22935779816513763Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG310.22219036697247707No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT310.22219036697247707No Hit
GCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTAT290.207855504587156No Hit
GCTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGG290.207855504587156No Hit
GGCGTTGATAGGCTGGGTGTGGAAGCGACCCTTCAGGGGGCTCAAGGCCC280.20068807339449543No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG280.20068807339449543No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC260.18635321100917432No Hit
GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT260.18635321100917432No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA250.17918577981651376No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA240.17201834862385323No Hit
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT240.17201834862385323No Hit
GATAAGATACACGGGAAACTGGCGTATAGATAGAATCGATCGGAATCACG240.17201834862385323No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA240.17201834862385323No Hit
AGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACG230.16485091743119268No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG230.16485091743119268No Hit
GCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCG230.16485091743119268No Hit
GAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCA230.16485091743119268No Hit
GTGCTTCCATCAGTCCAAACATCAAGTGTAAGTTTATCATAGTTTGCAAC230.16485091743119268No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG230.16485091743119268Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA230.16485091743119268No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG230.16485091743119268Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC230.16485091743119268No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG220.15768348623853212Clontech Universal Primer Mix Long (96% over 26bp)
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG220.15768348623853212No Hit
GTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCA210.15051605504587157No Hit
TGCTTGCGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTC210.15051605504587157No Hit
GTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGG210.15051605504587157No Hit
GAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATAT200.143348623853211No Hit
CTAGCATCTCGCACGGCAAGAGCGATTGCCGAGCTTAGAGCGTATCTTCC200.143348623853211No Hit
ATGGCACTCTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATC200.143348623853211No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC200.143348623853211No Hit
ATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCC200.143348623853211No Hit
CCTTTATATCCATCTATAACAACTGCGTCAGCATCACTTGTTGCTATTCC190.13618119266055045No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT190.13618119266055045Clontech Universal Primer Mix Long (100% over 23bp)
GTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCT190.13618119266055045No Hit
ACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTG190.13618119266055045No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT190.13618119266055045No Hit
TATCAACGCAGAGTACATGGGACTGCCCCCTTTCAGCCTTTTGGGTCCTG180.12901376146788993No Hit
CCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGA180.12901376146788993No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT180.12901376146788993No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC180.12901376146788993No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC180.12901376146788993Clontech Universal Primer Mix Long (96% over 26bp)
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA170.12184633027522937No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC170.12184633027522937No Hit
TGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACAC170.12184633027522937No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA170.12184633027522937No Hit
CAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGC170.12184633027522937No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT170.12184633027522937No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC170.12184633027522937No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA170.12184633027522937No Hit
AGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGC160.11467889908256881No Hit
GTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGTACAAAG160.11467889908256881No Hit
GCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTT160.11467889908256881No Hit
GTGGAGGAACTGGTCCTATTCGGCCGTAGCCTATGAGGGGCGGGGCTTCG160.11467889908256881No Hit
GGCGGATGCTCACGTCGCGGATCTGCGCGGCGAGGTCCGGGCCAATGCGG160.11467889908256881No Hit
GCGCGTATCATATAGGATGCGGCGATCTCATACAGCGTTACGGTCGCTGC160.11467889908256881No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT160.11467889908256881No Hit
ATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTG150.10751146788990826No Hit
GGTCAGGGCTGGCTGTCGGTTGGTTTTTTGGCTGCCAAGGCGCTTGATTC150.10751146788990826No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC150.10751146788990826No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT150.10751146788990826No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC150.10751146788990826Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT150.10751146788990826No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG150.10751146788990826No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG150.10751146788990826No Hit
GCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACT150.10751146788990826No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA150.10751146788990826No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC150.10751146788990826No Hit
TTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTG150.10751146788990826No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT150.10751146788990826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10751146788990826No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA140.10034403669724772No Hit
TTGCTATTCCAACAGCAATAGCTGGAGCATTATGGACAGCTGCAATTTTA140.10034403669724772No Hit
CTCTAACATTTGATGCTCTAACTCTGACTTTTTAGGGTGGCATATCTGTA140.10034403669724772No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA140.10034403669724772Clontech Universal Primer Mix Long (96% over 26bp)
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC140.10034403669724772No Hit
ATGGGGGAATTCTCCAGATTACTTCCATTTGCGCCCAAGCTGCTCACAGT140.10034403669724772No Hit
CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA140.10034403669724772No Hit
CCCTTATGCAGACCACATAATTACCCAAGTTGCAAGTGGAAGATTTGGAG140.10034403669724772No Hit
GTATCAACGCAGAGTACATGGGACGGCCGATTGTCGCAGCCCGGGTCGAT140.10034403669724772No Hit
TGTTAAGACACACTGCCCCAATCTCTCTAACATTTGATGCTCTAACTCTG140.10034403669724772No Hit
GAGTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG140.10034403669724772No Hit
AGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCT140.10034403669724772No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA140.10034403669724772No Hit
GTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCC140.10034403669724772No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC140.10034403669724772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)