FastQCFastQC Report
Wed 8 May 2024
EGAF00003673121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4640361
Total Bases464 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA123130.26534573495467273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG69680.15016073102933156No Hit
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGG60340.1300329866577191No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59330.12785643185950404Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAGAGTCGCCCTGGCATGAGTCCCTCCGGGTGTTCCCGGCACACAGC50590.10902169033831637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)