FastQCFastQC Report
Tue 10 May 2022
EGAF00003673140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276401
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71022.569455247991143No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41311.4945676752254877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18020.6519513315798423Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16630.6016620779230176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16050.580678072800026Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11700.42329803437758906Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9760.35311015517309996Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8650.3129511108859953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.3100567653517896No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5920.21418156953122458No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5760.20839287846281307No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5450.19717728951776586No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT5260.19030321887402724No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5210.18849425291514865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.18813245972337292No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4960.17944942312075574Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4640.16787204098393277Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4420.159912590764867Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4200.15195314054580122Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.14580265628561404No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3900.1410993447925297No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3860.13965217202542682Clontech Universal Primer Mix Long (96% over 26bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3860.13965217202542682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3740.13531065372411824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT3730.1349488605323425No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3410.12337147839551955Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3370.12192430562841669Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3350.12120071924486525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG3340.12083892605308953No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG3310.11975354647776237No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3130.11324126902579948No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3100.11215588945047232No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT3000.10853795753271514No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC2840.10274926646430367No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2820.10202568008075225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)