FastQCFastQC Report
Tue 10 May 2022
EGAF00003673249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58471
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9121.55974756717005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4300.7354072959244754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3640.6225308272476955Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3270.5592515948076825No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2560.4378238785038738No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2270.38822664226710674Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2060.35231140223358587Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1850.316396162200065No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1700.29074241931897865Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1600.27363992406492105Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1500.2565374288108635No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1460.2496964307092405No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1340.2291734364043714No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1310.22404268782815415No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT1310.22404268782815415No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1290.22062218877734263No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1250.21378119067571957No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT1250.21378119067571957No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC1190.20351969352328503No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1170.2000991944724735No Hit
GCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT1130.1932581963708505No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1120.19154794684544474Clontech Universal Primer Mix Long (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1110.189837697320039Clontech Universal Primer Mix Long (96% over 26bp)
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG1100.18812744779463322No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC1100.18812744779463322No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC1070.18299669921841596No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1000.17102495254057568No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG980.16760445348976416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.16760445348976416No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG970.16589420396435842No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG950.1624737049135469No Hit
GGATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT940.16076345538814113No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT930.1590532058627354No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT920.15734295633732961No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG920.15734295633732961No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG900.1539224572865181No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA880.15050195823570658No Hit
ACGTTATAACGGCCACTCAGTGGTTCGTCACGCGCAGCCCCGGGTTCGTC880.15050195823570658No Hit
TTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGACGCGGC870.1487917087103008No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG870.1487917087103008No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT850.14537120965948933No Hit
GTACATGGGCACGTGGTGGATGCCTTGGCAATGATAGGCGACGAAGGACG850.14537120965948933No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC840.14366096013408355No Hit
CCTTTAGGCTAGGCCTTATTAACCTCTCTCTCCTACATTTGCTATGCGTC840.14366096013408355No Hit
GACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAG830.1419507106086778No Hit
AGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAG830.1419507106086778No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA820.14024046108327204No Hit
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG820.14024046108327204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCT800.13681996203246052No Hit
CTCATCCCCAGGTTGTTATGGTCTACCGTGGAAGCCTCGTCAATGAATAC800.13681996203246052No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA800.13681996203246052No Hit
CTTTAAGCAGTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCA790.13510971250705478No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT790.13510971250705478No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT780.133399462981649No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG770.13168921345624326No Hit
GAACCATGCAGGCGGCCTCGAACCGATGCATGGCGACGAACGCGTCCGCG760.12997896393083752No Hit
ATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCA760.12997896393083752No Hit
GTACATGGGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACC750.12826871440543175No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG740.126558464880026No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA740.126558464880026Clontech Universal Primer Mix Long (96% over 26bp)
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA730.12484821535462025No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA730.12484821535462025No Hit
GAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGT720.12313796582921449No Hit
GAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATA710.12142771630380873No Hit
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA710.12142771630380873No Hit
AGTGGGAAGTGCTCACGCGCGGAGCCCACTGGGCGAACAGCAACGTTATA700.11971746677840298No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA700.11971746677840298No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC690.11800721725299722No Hit
GATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAG690.11800721725299722No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG690.11800721725299722No Hit
CAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGC680.11629696772759146No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC670.1145867182021857No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT670.1145867182021857No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT670.1145867182021857No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG670.1145867182021857No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT670.1145867182021857No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC670.1145867182021857No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC660.11287646867677995Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTC660.11287646867677995No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT640.10945596962596844No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT640.10945596962596844No Hit
GGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCA630.10774572010056266No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA630.10774572010056266No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG630.10774572010056266No Hit
GTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGA630.10774572010056266No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT630.10774572010056266No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC630.10774572010056266No Hit
GTACATGGGAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGAT620.1060354705751569No Hit
CTCTCCTACATTTGCTATGCGTCTCAAGATAAGGATATTATCTCATATGG620.1060354705751569No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC620.1060354705751569No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG610.10432522104975114Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG610.10432522104975114No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA610.10432522104975114No Hit
GCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGG610.10432522104975114No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT600.1026149715243454No Hit
GAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCG600.1026149715243454No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT600.1026149715243454No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC600.1026149715243454No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG600.1026149715243454No Hit
CAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGC590.10090472199893964No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC590.10090472199893964No Hit
GTACATGGGCGACCAGCGCTGACGAGCTGGCGCAATGACGACCTAATTGG590.10090472199893964No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC590.10090472199893964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)