FastQCFastQC Report
Tue 10 May 2022
EGAF00003673250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58471
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24314.157616596261395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5130.8773580065331531Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG3540.6054283319936379No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3460.5917463357903918No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3180.5438593490790307No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT2580.44124437755468526No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2520.43098288040225063No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2410.41217013562278737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG2400.4104598860973816No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC2390.4087496365719759No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2370.40532913752116434Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2160.3694138974876434No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT2070.35402165175899164No Hit
ATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCA1970.3369191565049341No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1940.33178840792871683Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA1920.3283679088779053No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1920.3283679088779053No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1880.32152691077628226No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT1610.2753501735903268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.266798925963298No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1520.25995792786167504Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1510.25824767833626927No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1460.2496964307092405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1390.23772468403140018Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1380.23601443450599444No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1260.21549144020112532No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1240.2120709411503138No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA1210.20694019257409654No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1150.196678695421662No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG1120.19154794684544474No Hit
GCACAGATGCGAGCCCTCGTAATGTGCATCAGCTAAGGGCGATATTATAA1100.18812744779463322No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC1090.18641719826922748No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG1060.18128644969301022No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1060.18128644969301022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1050.17957620016760445No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA1030.17615570111679293No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1010.17273520206598142No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG980.16760445348976416No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT920.15734295633732961No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT920.15734295633732961No Hit
GTACATGGGCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGC920.15734295633732961No Hit
CCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCC910.15563270681192387No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT910.15563270681192387No Hit
CCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATG900.1539224572865181No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA900.1539224572865181No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT890.15221220776111233No Hit
GACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGA870.1487917087103008No Hit
GTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGCCGAGCTTA870.1487917087103008No Hit
GCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGT860.14708145918489507No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT860.14708145918489507No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG850.14537120965948933No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT850.14537120965948933No Hit
GCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCC840.14366096013408355No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT830.1419507106086778No Hit
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA830.1419507106086778No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA820.14024046108327204No Hit
CCCTGGCACAGAGCACTTCCTGATCAACCCCTACGGGATGATGTTCGACG820.14024046108327204No Hit
GTACATGGGAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGAT810.1385302115578663No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA800.13681996203246052No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC790.13510971250705478No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA780.133399462981649No Hit
GCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGA770.13168921345624326No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC770.13168921345624326No Hit
CATATAACCACATTCAAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA750.12826871440543175No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA750.12826871440543175No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA750.12826871440543175No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT740.126558464880026No Hit
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA740.126558464880026No Hit
CGTTTATTGTAAGCCGTCGGCATCGGGATATCATCTGCTTCAAGCTCCTC730.12484821535462025No Hit
GAGTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC730.12484821535462025No Hit
GAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATA730.12484821535462025No Hit
GTGGATGCCTTGGCAATGATAGGCGACGAAGGACGTGATAGCCTGCGATA730.12484821535462025No Hit
GCTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGC730.12484821535462025No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG720.12313796582921449No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC720.12313796582921449No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA710.12142771630380873No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT710.12142771630380873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.12142771630380873No Hit
GTACATGGGTTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGAC700.11971746677840298No Hit
GGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGC700.11971746677840298No Hit
TTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGG690.11800721725299722No Hit
CCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGCCGAGCTTAGAGCG690.11800721725299722No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC680.11629696772759146No Hit
GGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCC680.11629696772759146No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA680.11629696772759146No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA680.11629696772759146Clontech Universal Primer Mix Long (96% over 26bp)
ATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCA680.11629696772759146No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG670.1145867182021857No Hit
TTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGAT670.1145867182021857No Hit
TCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGG670.1145867182021857No Hit
GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG670.1145867182021857No Hit
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA670.1145867182021857No Hit
CCCTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAA670.1145867182021857No Hit
CTTGTTGTGCGCTCGTATCAGGCTATTCCTATAGCAGTTCACGACCGAGC660.11287646867677995No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG660.11287646867677995No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG660.11287646867677995Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG660.11287646867677995No Hit
TATCAACGCAGAGTACATGGGACATGTACGGAATTTGCCAGAGATGGCTT660.11287646867677995No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG660.11287646867677995No Hit
GTACCACACAGGTCATTTCACGTACGGGGCTATCACCCGCTATGGCCAGC650.1111662191513742No Hit
AGCCAAACGCGCCCGATGAGACGATCACCTCACGGCGCGCGCGCAACGTT650.1111662191513742No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT650.1111662191513742No Hit
GTACATGGGAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCA650.1111662191513742No Hit
GAATAAGAGCACGTGGTGGATGCCTTGGCAATGATAGGCGACGAAGGACG640.10945596962596844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG640.10945596962596844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC640.10945596962596844No Hit
GTACATGGGAGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC640.10945596962596844No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA640.10945596962596844No Hit
CGATCGAGGAGCGCAACATGGTGAGCGGGGTGCTCTCGCTGGCTGAGCGC640.10945596962596844No Hit
GAATTGGGGACATTCAAATGAGCATCCTTCACCGCGTTTAAAATGAACCT630.10774572010056266No Hit
GTCATATTAACAACCGTTGCGACGGGTCCGAGACATTATACACTTATGTA630.10774572010056266No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA630.10774572010056266No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC620.1060354705751569Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCTACATCGCGGCTATCATTAAAAATTCCCCTTTAACAAAGGTTGTGG620.1060354705751569No Hit
GCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGC620.1060354705751569No Hit
ACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAG620.1060354705751569No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA620.1060354705751569No Hit
ACAGGGTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTA610.10432522104975114No Hit
GAGTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCT610.10432522104975114No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA600.1026149715243454No Hit
CCATAACACGATCTAGTGAAACCTTCAAGCATGAATTGTAGCTGACGGCA600.1026149715243454No Hit
ACCCAGGCCGTAGATTGAAGACACCGAAGCGACGACGATCACGTCACGAC600.1026149715243454No Hit
CAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGC600.1026149715243454No Hit
TATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCA590.10090472199893964No Hit
GTGCAAACCCGAGAAAATCGTCTACATCGATGAGCTCGACCCCAACGACT590.10090472199893964No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA590.10090472199893964No Hit
TGATCACACGCTGTACCGGCTTGACCCCGGACTTCTTGCTCTGCACTGCG590.10090472199893964No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA590.10090472199893964No Hit
GGGTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACC590.10090472199893964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)