FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003673305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTGCCCTGTGTGATCCTCCACGTTTTCCTGGAATTAGGAGAGCCCAC318.75No Hit
GTCGATGAGCTGGTGGCGGACGTTGTCTTTCCACCACTGCACTCCGCGCG212.5No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG212.5Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGACCCGGTTGAGGCTGAACGCGAGGCTATCGAGCGTGAGCTGGAG16.25No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT16.25Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGGGCTGCGATGGTGGCCAGGTCATCGGTGGACTCGACGAGGACTA16.25No Hit
GCAGCCCGGCAGAGCCGTTGCGCCACGCGTGGCACGCCCTGGTCTCCGAG16.25No Hit
ACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCG16.25No Hit
CCCGCACATAGCCGCAGCAGGGTAGTTTTCCCCGCTCCATTAAGACCCAA16.25No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16.25Clontech Universal Primer Mix Long (96% over 26bp)
GGGTGGCCAACGGCGAATCGGACATCGGCTTCTGCCTGCGCCGCAAGCCG16.25No Hit
GGTCTGATCAACGACCCTGACCTGGACGGCTCGTTCAAGATCAACTCGGG16.25No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)