FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003673306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCACCCAGGCTCCAGAGCCACAGCCTTCTAATCTCACGGTTGGTCTT318.75No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT212.5Clontech Universal Primer Mix Long (96% over 26bp)
CTATTACCGCGATGTGGCAGAGGCGGGGATTTTTGTCACCAACTCCCGGA212.5No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTCGATCTGACTGGGCAAGAGGAAGCAGCACTAGAAGTCATTGGCCCG16.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT16.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTAGCCTCATGAAGAATCCAACGGCTGCAAGCCTGACATTAGCGGTCG16.25No Hit
TGGTTAGTCGGTTCGTCGAGCAGCAGCAGATTCGGCCGCTGCCAGACAAT16.25No Hit
CGGCTGACTCGATGCGCGGATCGCATCGACCGCCACGCCAATGCCACCGT16.25No Hit
CTTCAGCAGTCTCAACTACAGGCTCTTCAGCAGCATCGGCAGCTACCTCA16.25No Hit
CTCCACCCGAGGCGCTCGGCCATCTCGCCGACGCGGGTGGCCCCGCCCTC16.25No Hit
TTCCACCAGCGGGCGCACCTTGCGGTGCTCGCGGTGGTCGATCTGGTCGA16.25No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)