FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCGCGCTGCCCGAGCGCTACCGGCTGATCCTGTGCGACCTGTGGGGCT43.3057851239669422No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC32.479338842975207No Hit
GTTGTAGGTGACGAGGCAGCCGGTCTGTCCGATGCTGCGGCACACCGGGA32.479338842975207No Hit
CGCGAGCACGATGCCGGCCTCGGCTCCGCCCTCGCCGAACGCCGACACGA32.479338842975207No Hit
AATCAGCCTTATGCATATGATGCTTATGAAGATGAAACAGATAAAATAGT32.479338842975207No Hit
GCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGAC32.479338842975207No Hit
GTCTTCAAATTTTGGACCCATGAAGGGAGGAAATTTTGGAGGCAGAAGCT32.479338842975207No Hit
GCGATATCATCGCAACGACGCTGGCAAACCGGTCTGGCGTCCTAGCCGAT21.6528925619834711No Hit
TTATTAAACAAACTGTCACAATGGACAAACTGCATGCCTTTCCATGAATG21.6528925619834711No Hit
ACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTA21.6528925619834711No Hit
CCACTGCACCTGGCCGGTATTTCTGTGGGAAAGTTGCTCTTCACCTATTG21.6528925619834711No Hit
TATCAGTCTAGAGTTATCCAGGGCCTCGTGGCCGGAGAAACTGCCCAGCA21.6528925619834711No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA21.6528925619834711No Hit
GATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTC21.6528925619834711No Hit
CGTATACCCAGCTGGTAACTGAGTGGCTCGGTCAACGTGAGTACCAGGAA21.6528925619834711No Hit
ATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCC21.6528925619834711No Hit
AACCGGCAGCGGCAGCGAGTGGAAATTAAACCCAACCCAAGCGGCCAATG21.6528925619834711No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC21.6528925619834711No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCA10.8264462809917356No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC10.8264462809917356No Hit
GCCTACTTGCCGCCGATCGTCAGGCTCTCCGCAGGCGGTGCGCCCTCGGG10.8264462809917356No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10.8264462809917356No Hit
ATGTATCTCATGAAAGGAAGGCTCTGAATCTTGTAGTGTAGAAAACCACA10.8264462809917356No Hit
AACAACGTGCGTACGTACACCCCGAAGCCCGGCGACGCCGATCGCCAGTG10.8264462809917356No Hit
ACCCGGAGGTGCTGGGCCCTGAGCTTCTACCCTGCGGAGATCACACTGAC10.8264462809917356No Hit
AGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATT10.8264462809917356No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG10.8264462809917356No Hit
CGCCCAGCTCGTGGCCCTGCTCGGCGACGGGCTGTGGCTGCGCTCGGCCC10.8264462809917356No Hit
AGAAGAGACGATGGAAGAGAGAATGTCATTAGAGGAGACCAAGGAACAAA10.8264462809917356No Hit
GTTTTCCGGCCATTTAAAGGGGAGGTCGTGGATGCTGTTGTCACTCAGGT10.8264462809917356No Hit
ATCCACCACAGATGGCCGCAGTGGTAATGAAATAATTCTAGCTGGGTCCA10.8264462809917356No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10.8264462809917356No Hit
TTGTGATTCATGGCGACGTGGAACCGCTTTGCGCACGACCACGATGACGT10.8264462809917356No Hit
TGCCCATATTTATCACCAGCACTTTGATTCTGTGATGCAGCTGCAAGAGG10.8264462809917356No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10.8264462809917356No Hit
GGCTTATGATGTGTAGCTGGAGCTGCACTGACCCAGACGATCTCCCCAGC10.8264462809917356No Hit
CAAGTGGACTTGGTGCTTCTCTTTGACAGCCAGGATTGAAAAGCCTAGTT10.8264462809917356No Hit
GCGACTCGTCAGGCACGTAACCCCACAGGTCCCGACGCACCCGCGCATGC10.8264462809917356No Hit
TCTCTGAGACACAGCAAAGCCATGTTAAGAGTAAAGTTTTGGCTGGGTGC10.8264462809917356No Hit
TTCCAGGATGAACCGACGACAACGCCTAACCAACCGAACTCGCCTTGAGG10.8264462809917356No Hit
CCGAGCTGCTGGGCCGCTCGCCCGCGTACCGGTTCATCATCTCCAGCCTC10.8264462809917356No Hit
TCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAA10.8264462809917356No Hit
CCAGAGAGCCTACCTGGAAGACACATGCGTGGAGTGGCTCCACAAATACC10.8264462809917356No Hit
AGTCAGCTCCACTGAAGCTGTGGCCCGGGATGCGGCTCGTAGCATCCGCA10.8264462809917356No Hit
GCAGATGTCCCCGCCCATCTGGAGGTCCGGGCGGGTTCTGCCGGCCGCGC10.8264462809917356No Hit
TAAAGAAGAAGATTTCAGCCAGGAACCGGCGCAAGAGTCGCACGAATTCA10.8264462809917356No Hit
GATCCTGGTATCCGCTAACAGGTCAAAATGCAGATCTTCGTGAAAACCCT10.8264462809917356No Hit
CCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG10.8264462809917356No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG10.8264462809917356No Hit
GGCTAACTGTCCTTGTCTCAGGAGCCCTACCTGGCCAGTCTTGGGCCAGG10.8264462809917356No Hit
ACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGT10.8264462809917356No Hit
CCATAGCTTGTTGCAGAAGTTCCTATACCCTTCGCAACGCTTTAAATCGA10.8264462809917356No Hit
ACTCTCGAAACAGCCGGGCCCGGAAGGCCCCCCATTTGTCCAGCAGGTCA10.8264462809917356No Hit
GTACAGAGCAAGAGGATAGCTGAGACCCAGCCCAGGTAGAAGGACCAGGA10.8264462809917356No Hit
CCTTAATATCGCCGAGCTCGGAGGTGGATGCTGACACCAGGCTTTGCAGA10.8264462809917356No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT10.8264462809917356No Hit
CAAGGAAGAAACAGCGGGTCTGAACAAGGGCAAGGATGCGAAGGCCGAGA10.8264462809917356No Hit
GCATGATGCCGATGCTTCCCAGCAGCCCAACCATGCCGAGCACCATGCCG10.8264462809917356No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10.8264462809917356No Hit
CACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTA10.8264462809917356No Hit
GAGAAACTGGAATCATAATACACTGTAAAATCAGGTATGCAAAAGGCTAT10.8264462809917356No Hit
GTGCTGAGCCAGGGTTATGTCACAATCTTCCCTGACAGCATGCACCAGCA10.8264462809917356No Hit
ACCAAAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTAC10.8264462809917356No Hit
ACTAAGGACTTCTGTAGCGGCCTCTAAATCTTCGATCTCGACTTCTTGAG10.8264462809917356No Hit
TTCCAACAGTTGTTACTACATTGGTAAGGAAAGAAGAACTTGGGAAGAGA10.8264462809917356No Hit
GCTCCCCCATCCCAAAGAATTAAGCAAAAGAACATCAAAATAGAGACAAC10.8264462809917356No Hit
CATTCCAGGGGACTGGGGACTGTTCTTCCCCACTTCTGATGGCCTTCAGC10.8264462809917356No Hit
GCGTGGAACGGGAGCCGGAGTCTGGCGGTGGCCGGCTGGTCCTGGACGCC10.8264462809917356No Hit
TCCGGGGCCTCAGGCAGGTGCGCCCGGTGCATCGCGACCTCATGCACGTC10.8264462809917356No Hit
ACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGC10.8264462809917356No Hit
TCTCTCAGTTGCATCTTAGTAGCCTTAAATTACAAGTAGATTATATCACT10.8264462809917356No Hit
GAAAATGCAGACCCGCAGGGCAGGAATTCCGAGTCCGGGCTGGAGCGCGA10.8264462809917356No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC10.8264462809917356No Hit
GTCATTAAATCTTAAAGCTCTAAAATCTCCTTTGACTCCATGTCTCACAT10.8264462809917356No Hit
TGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTC10.8264462809917356No Hit
GTCGTAGCCGATGCGCTTGATGGTGTTGCGCGCGACCTCGATGTAGTCGA10.8264462809917356No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC10.8264462809917356No Hit
CAGGTAGTCCGGGTCCGGCAGGGACTCCGGCCGGTGCAGGTCCCACGCCC10.8264462809917356No Hit
GACCAGGACTACGTCTTTATGCCGACTCACTCCGCAGGTCCATCGCCAGA10.8264462809917356No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG10.8264462809917356No Hit
CATCTATCTGGCCGTGTTCGGCTGGCTGATCGTGGTGTTCGACAAGTCCG10.8264462809917356No Hit
AAGCCCACCTCAATCATCAGGTTTTCGTCTGCAAATGCTGCAGCAATCAG10.8264462809917356No Hit
GTCTTGGGTCTTGGCCTTATTGTAGGGAGCTTTTCTATCATAGTTCACTT10.8264462809917356No Hit
AATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGG10.8264462809917356No Hit
AGTGTAATGGCGTAATCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCA10.8264462809917356No Hit
CGCAGCCCCGGGTTCGTCCCCTATAAGGGCCTAGTACCTTTCGAGCCCCG10.8264462809917356No Hit
ACGCTCCACCCCGTCACCTCCCTTACACCCTTTTCTCCGCTTCTCCCCAC10.8264462809917356No Hit
GCCGTGACCAGCACCAACATCCGTGAGCTTCTTGTCGAGACCCGCAGCTT10.8264462809917356No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACC10.8264462809917356No Hit
GAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGG10.8264462809917356No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA10.8264462809917356No Hit
GGCTCACACCTATAATCCCAGCACTGTGGGAAGCCGAGACAGGTGGGTCA10.8264462809917356No Hit
GTTTTCTATGTGCTTACTAATTTACCCCCAGACTCTTCCCAAGCTGTCTG10.8264462809917356No Hit
CACGCGGAGCGTTCGACCGGGGATGTCGAGCACCTGGTCGTCGTCGAGCA10.8264462809917356No Hit
CTCAAGGAAGTGGGGCTTCACCAAGTTCAATGCTGATGAATTTGAAGACA10.8264462809917356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)