FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGTGGCGGCGCTGCGGGGGGCGTTGGTCACGAACAGCACCGTCCGG43.3057851239669422No Hit
CCTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGG32.479338842975207No Hit
GAGCTCGCCTACGCCGACGTTCTTGCCGCATGGAAGCACTACCTGGCCCA32.479338842975207No Hit
GTCCCGTCATCACCACGTTCGTCTCGGTGCAGATCGGCACCGTGTGGGGC32.479338842975207No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA32.479338842975207No Hit
GTATTTGTCTAAAACATGTCTTCTTTGTAGCAGCAAGGCCCTGCCACCAC32.479338842975207No Hit
CATCAAGGGCACTGTTTGGTTTTTCATTTGCTCTGTAAAATTATTATGGG32.479338842975207No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA21.6528925619834711No Hit
GCCCTGTTCCAGGGAGCTTTCGGATCCTGGCAGGCTCTTACTGCGACGAC21.6528925619834711No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC21.6528925619834711No Hit
GGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAA21.6528925619834711No Hit
GGAATATTCTTTATATACTTCAAATTTAGAACTTAACTTTAAAAGTTTTT21.6528925619834711No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT21.6528925619834711No Hit
CAACGCAGAGTACATGGGGGATTAGACCGTCGTGAGACAGGTTAGTTTTA21.6528925619834711No Hit
ACTGTAATCTCTGCCTCCCTGGTTCAAGCTATTCTCCTGCCTCAGCCTCC21.6528925619834711No Hit
GGTTTAGGTAACTGCGCAGCAACCTGCTCCACCTTCGCGGATTTTAGTTC21.6528925619834711No Hit
GGTTGGCGGTGCCATCGCCGTAGATATCGGTACTGATCTCGTCGACAAGC21.6528925619834711No Hit
GATGTGTGCTGGTGAATGTGAGTACAGGGAAGCAGCGGCCGCCATTTCAG10.8264462809917356No Hit
GCACAAGCGGCCAAGCAAGCCGCTGCTGCGCCTTGTGCAGCAGCGGCTTG10.8264462809917356No Hit
GTCACTGCTTGCAGACTGAGAGTAGATCCCTACTTTAATAAATTAGCTCT10.8264462809917356No Hit
ACATGTAGCATTGCACAGTTACGTTCAGCATGATCTGAGTCTTTTATCTC10.8264462809917356No Hit
GCGGCCGAGGTGATCGACTTGCCGAGGCCGCCGACAGGCCCGCCGAAGTA10.8264462809917356No Hit
GATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTT10.8264462809917356No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG10.8264462809917356No Hit
CCGGTCCACGCAGTTGACGGCGACGTTGAGGCGGCCGCCCGCGAACCACT10.8264462809917356No Hit
ATAGAATATTATTTGGATATACCACATTTAATTTATCTGTTCATTAGTTA10.8264462809917356No Hit
ACATCCTGGTCGGGGAAGTCCAGCACCCGGTCGATGAGCACGGACAGCTC10.8264462809917356No Hit
GAGCCGTCCTCCTCGGTTAGAATCCCACCTCAACTGACTAGCTGTGGGAC10.8264462809917356No Hit
GGTACGGGCAATTCTCTGCCTCAGCCTCCTGAGTTGCTGGGATTACGGGC10.8264462809917356No Hit
TGGACGCCTTGCTCGAGAAGCGCACTAGCGGGCTGATGAATCCAAAGCAG10.8264462809917356No Hit
GGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCCGGGGCGTGGCGCCACAAG10.8264462809917356No Hit
AAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAA10.8264462809917356No Hit
CATTATACACTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAAT10.8264462809917356No Hit
GTCCCCACTGTGGCACTGCCTAGTGGAGCTTTGAGAAGAGGGCCACCATC10.8264462809917356No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10.8264462809917356No Hit
AGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGC10.8264462809917356No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG10.8264462809917356No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT10.8264462809917356No Hit
AACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACAT10.8264462809917356No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC10.8264462809917356No Hit
ACATGAGAGAGAGATGACAGATGCCCCAGCTGATGGTGAAAGAGCACAGG10.8264462809917356No Hit
GTTCAATGCTGATGAATTTGAAGACATGGTGGCTGAAAAGCGGCTCATCC10.8264462809917356No Hit
CCCCTGCATGCTGAGGAGATGAGTTCCTGTGTGAACAGTCAGGCTCTGCT10.8264462809917356No Hit
GCCGATGAGACGAGCAGCGGTTCGCGGAGGAGCACGTCGCTCAGGGCGTG10.8264462809917356No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGG10.8264462809917356No Hit
AAGTACCTGTTCCCTGCCGCGATGTTCTTCATTGCGACCGGCGTGTACAG10.8264462809917356No Hit
TGGCCGACTATATCGCGCCAAAGTCCATCGGAGTCGCCGATTACATCGGC10.8264462809917356No Hit
GGAGTCGCACCGGCCGTACCTGGACGCGCTCGGGGTGACGGCGGCGGAGG10.8264462809917356No Hit
AGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTT10.8264462809917356No Hit
ATCAATTGATGCGGCACGCGGATCAACGGTCTCAAGAGGCTGGGCGTGTG10.8264462809917356No Hit
ATTCTCATTCCAATCTCAAAAAATCAGGATTGCTATCTCTCCTTCACAGA10.8264462809917356No Hit
NGAGGGACCCAGTGGGAGATAATTGACTCATGGGGCGCTTTCCCCCATAC10.8264462809917356No Hit
GTACGGATGCATGACGATCTCAACCAGCGGACGGTAAAAGACAAAGGCCA10.8264462809917356No Hit
CCGCCGCCCGCAGCGGGCCGGTGTCCTCCGGCAGCTCGAAGTCCGTCAGG10.8264462809917356No Hit
GCCCGACGGGCTTTGGCTTGAACCATCGGAACGCGGGCTTTCTGTCACTG10.8264462809917356No Hit
TCCGTGAGCCCTTCCAGGGCCAGCTCGCAGGTGCGCAGGGCAGTGAGGGC10.8264462809917356No Hit
ATTTAATGATATTGGTACTTAAGATTTTCTGATGTAATGGTATGAGTATT10.8264462809917356No Hit
CCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCA10.8264462809917356No Hit
AACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATC10.8264462809917356No Hit
AAGGGTGTAAATTAGGATCCTCACTGAAGTGGCGGGACCCTAAGAGGCTT10.8264462809917356No Hit
GATCAGCCCCGTCGCAGCGCCGCCTCCAGCGCGGTCACGAAGCCGTCCAC10.8264462809917356No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10.8264462809917356No Hit
TACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAA10.8264462809917356No Hit
ACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTC10.8264462809917356No Hit
ACAATATCCTCATCCATTGTCTTGTAACATGGTGGGTTGGAGTTAGGATC10.8264462809917356No Hit
ACTCTGGCCCAGGCCTGTTCCTGAACCCCCAGTTACTGGAACCCCCAGGA10.8264462809917356No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA10.8264462809917356No Hit
GGCTCGGGGACGACTGGGGCTGGCGCGCCTCCTTCCTCGTCAACGTCCCC10.8264462809917356No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA10.8264462809917356No Hit
CATCATATCAGGCTACATCCACGTGACGCAGACCTTCAGCATTATGGCTG10.8264462809917356No Hit
GTTGATGATCAGGATGTCATTGAGATAGTGCAGGTGGTCTAGGTGCTCTG10.8264462809917356No Hit
TTGCTGCCATGATCTGTCGTAAGTTTTGTTCCAGTTACTTGAGAGTTAAA10.8264462809917356No Hit
GTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGT10.8264462809917356No Hit
CAAATGTTCTGTCCAATAGCTTGTACTATCCTAGGCTTACCCAGCCCTAA10.8264462809917356No Hit
GTTCCATCCCCTGCAGGCCTGGTCTCCACGAGCTCCGTGTCCTGGGTATG10.8264462809917356No Hit
GTCGAGAGGACGAGAACCTCCCGTTGCTGCGCGAATCTGGTGCCGACGCT10.8264462809917356No Hit
CTCTACTTCTACCTGCCTTCTTCCCATAGAGAAGATTGTGATATATTGCT10.8264462809917356No Hit
CCTCGCCCCGCGTGCCGAGCAGGGTGGGCGGCTGGCGGTCCTGGCGGCGG10.8264462809917356No Hit
CAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG10.8264462809917356No Hit
GGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGTCCCC10.8264462809917356No Hit
ACCAGGAGGCGGCCAGGAGGCTGGCCGAGGCGGAACCCAGGCGCACGGCC10.8264462809917356No Hit
ACCGCGGGCGTCTGGTCGACGAGGCCGAGAACCTCACCACCTTAGAGATT10.8264462809917356No Hit
ATTCTCATTCCAATCTCAAAAAATCAGGATTGCAATCTCTCCTTCACAGA10.8264462809917356No Hit
CCCCATGAAAGCATCACCATTAATCTGACCAGTCACTGCAGCATGAGTAT10.8264462809917356No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA10.8264462809917356No Hit
CTTCTGTTTCTCGTGGATTGTAACCTCCTGAAGGGATCCCACCAAGCCAG10.8264462809917356No Hit
CCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAAC10.8264462809917356No Hit
CAGGAGTTTGAGACCAGCTTGGCCAATATTGTGATGGGGTTTCACCAAAT10.8264462809917356No Hit
GTGTCACTGGGCTCCACTTCCAGGGTGATGGTCTTGCCGGTCAGGGTCTT10.8264462809917356No Hit
CGCACGGCCTTCTCCACGGCGCGCACCGGGTTCTTCTCCAGCAGCTCCGC10.8264462809917356No Hit
AGTTCTAGCAATAGATGAAGCAAGGGGGTAACTGTGTTCTAGAAGAAACA10.8264462809917356No Hit
AAGTCCATCTGCTCAATTCTCCACACTTTCCCAAGCTGGGAGATGGGGGA10.8264462809917356No Hit
TGCGTGTAGTGACGAGTACAGTTGCGTCGATTTAGGAAAAATCTTAGATA10.8264462809917356No Hit
GGCAACGCGAGTTGCAACTTGATGGACTGGAGTTTAAGAGTGGCGAACGA10.8264462809917356No Hit
ATCAAAGACCCGGCTGCCAATCTGCACATTTCGTTTTATGCGCAACGCAA10.8264462809917356No Hit
CCTGTGAAGTCCGTGCCCGTGTCCGCAAACGCCTGAGCCATGAGCAGGGC10.8264462809917356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)