FastQCFastQC Report
Tue 10 May 2022
EGAF00003673335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135998
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA6120.45000661774437856No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4720.3470639274106972No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT4070.29926910689863084No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT3660.2691216047294813No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA3040.22353269901027956No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT3040.22353269901027956No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC2810.20662068559831764No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2740.20147355108163356No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG2230.16397299960293532No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA2120.1558846453624318No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT2080.15294342563861232No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT2070.15220812070765746No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2060.1514728157767026No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG2020.14853159605288313No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1990.14632568126001852No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1950.14338446153619905No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1860.13676671715760524No Hit
GTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCT1810.1330901925028309No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1810.1330901925028309No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT1620.11911939881468846No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1610.11838409388373358No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT1610.11838409388373358No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1590.11691348402182386No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT1580.11617817909086899No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG1530.11250165443609464No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1520.11176634950513978No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1510.11103104457418492No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1470.10808982485036546No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT1450.10661921498845571No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1440.10588391005750084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.10588391005750084No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC1380.10147208047177166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)