FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAAGGTCATTGTTGCCTGTTTGAATCCAGAAAAAAATGCCTGGCCC24.3478260869565215No Hit
GCAGTGTCGTCCCGGCGTTATTGCGGTTATGGCACTCATCGCCCATATGC24.3478260869565215No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC24.3478260869565215No Hit
GTCTGGATCAAGGACTGGTCCGAGCACGAAGGACTGACCAAGGAGCTGGT24.3478260869565215No Hit
GTAAAATGTTGTTCCAAAAAATGTCAAATAAGTAACAAATTTATTTTCGT24.3478260869565215No Hit
GCCTGGCGTGGGCTCAGTTGTGCCAGCAGGCAGTGGTGGAGTTTGGGCTC24.3478260869565215No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG24.3478260869565215No Hit
GTTCCAGGCTTCCGGTCCTTAGCCTTCCCAAGTGGGACTTTAGGCATGAT24.3478260869565215No Hit
GAGAAGAACACGCTGAACAGCGCGGCCATGACAAAGACCATGATGGTGGT12.1739130434782608No Hit
TCCCAAGACCAAGGGCGAGTGGCGCGCGATGGAGTTGCCGGCCTGGAAGG12.1739130434782608No Hit
GTGCCCTGGTTGTTGCCTGCGAAGAGAGCGACTGCCGCCGCGACGGCGAA12.1739130434782608No Hit
ATTTTAGAGTGAAATCATCTCTCTTTTTTCTGGCAAATCTTTGAATTCCA12.1739130434782608No Hit
CAAACGTCTCCAGCAGCGACGACAGATTCGGGTCGTCAGGCCGAAGGTGG12.1739130434782608No Hit
TACTAAGCTCATTCTGGAGAAGACTTGTCAGGTAGGATAGAGAAAAGAAC12.1739130434782608No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG12.1739130434782608No Hit
GTTCCTCGACATCACCGGCTGCGCGCACTTGTTCGGCGGCGAGCGCGCGC12.1739130434782608No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTGTG12.1739130434782608No Hit
CAGGATGACTTACTCAAGAAATAGTCCAATCCTGTTTTTTCTTGACTGAG12.1739130434782608No Hit
TGACCACCGATCTCCTCGGCGGGGCCGCTGCCCTGCGCCTGGCACCATCG12.1739130434782608No Hit
GAATGAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGG12.1739130434782608No Hit
CATGATGTCAGGGACAAGCCTGTCAGGCGGTCAGATACCCGTGCCCCGCA12.1739130434782608No Hit
GGTGCAACTGAGCGTCGGCGACGACCCGGCCGCGAACCTGCCCGTGACGC12.1739130434782608No Hit
GGTCGCTGGAGTGCTCTTCTAAAACCAGAGCCCCAAGAGGCCCTGGGCAA12.1739130434782608No Hit
ATCCGGGACTGGTCGGTGTCGCCCTGCCACAGCCCGGCCAGCACATCGTC12.1739130434782608No Hit
TCCGGAGGCTGAATGGACTTGAATCTCGGCGACCCCCCGAGAACTCAGCT12.1739130434782608No Hit
CCATGAAATTGTTTCGGCGGTTCGATATCGATCTCAAGCTGGCCTACCCG12.1739130434782608No Hit
GGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCG12.1739130434782608No Hit
CTCTTATCCTCACTCCCTGGTGCCGCTGTAACATCAATCCAGATAGATGG12.1739130434782608No Hit
CTATCATACAACTGATCACCCAGGCATTTAAAAGTGCATAACGACACAAG12.1739130434782608No Hit
CTACTAAAAATACAAAATTAGCCGGGCGTGGTGGCGCAAGCCTGTAATCC12.1739130434782608No Hit
ATGGGATACAAATCGGAAATTACAGTTCCTAGTCCGTATTCATCCACTCA12.1739130434782608No Hit
GTGTGAATCAGTGCCGAGACATCTTTCGGGAAGCAGGAACCACCCCAGCC12.1739130434782608No Hit
CTGTCGAACAGGGTGATCTGGCGCTCGTCGGTGCGGCCGGGGGCCTGCCC12.1739130434782608No Hit
GGTATATTGTTTTTATTTATGTCTGAGGCTCAGTGAAAACCAACAAGTCT12.1739130434782608No Hit
ATTATTTGCTATACCCCAAACGGTGATGCTTATGAGTTGCCGCGCGGCTC12.1739130434782608No Hit
GTGTCGAGCCAGGTTCCGTTGTGCCAGTCGGCCGCCTCGGCGAAGAACAT12.1739130434782608No Hit
CAGGAATACTTAAGAGGATTGGCAAACCGGAGAAACGCTGGGAGAGGTGC12.1739130434782608No Hit
GGACCGCAGTCCCCCGAACAGGGTCTGTCGGCAACGATGACCGATCCCGT12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)