FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTGTAAAGGCTTCTCTTCACAAAGAGTCTAAATAGGACATTGAAT25.405405405405405No Hit
ATCGACCGCGTCGCCAGCCGGCTCGGACGCAGGCTGCACGAGGTGCCGGT25.405405405405405No Hit
TCAGAGATTTGTTCTTGTAACTTTTCAGGCGAAAGTTCTCTTTTCCTGGG25.405405405405405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTAACTTTTTTTTTTTTTTT25.405405405405405Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTACCGCCATGGGCCTCACTATCTCCTCCCTCTTCTCCCGACTATTTG12.7027027027027026No Hit
GAAGGCTCAAGCACAGCCAGTTATGTAAATGTATCTATCCCAATTGAGAC12.7027027027027026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG12.7027027027027026No Hit
ATTTTGTGTCAACTGGAGTTTTTTACAACTCAGGTGAGTTTTAGCTTTAT12.7027027027027026No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC12.7027027027027026No Hit
GTCCCTGCCCAGGGACGAGCGCTGCAACCTGACGCAGAACTGCTCACATG12.7027027027027026No Hit
GGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACT12.7027027027027026No Hit
GGGTGAGTCTCAAGCCAGTGGACCAGTGAGGGGTGAGGGCACGTCCTCCG12.7027027027027026No Hit
GTGTACTTAGCTGCACTTGGAAATCCGTCCAGGTTTAATAAGCAAATACT12.7027027027027026No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA12.7027027027027026No Hit
AAATAGAAATTGGTCTCCCACCTCCCAGCAGTTTTCAGATTCTAGATTAG12.7027027027027026No Hit
CACCTGTAGTCCCAGCCACTCGGGAGGCTGAAGCAGGAGAATCGCTTGAA12.7027027027027026No Hit
GCTGTAGGGTGTGTGGTTAAGGTACAACCACCCCTAAAGCTCAGTTTTGA12.7027027027027026No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGTCGGCCTCCCAAAGTGCTAGGATTACAGGCGT12.7027027027027026No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC12.7027027027027026No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
AGCGCAACCCCTATTGTTAGTTGCCATCATTTAGTTGGGCCTGTCTCTTA12.7027027027027026No Hit
TAAGTGTACAGTTCTGTGGCATTAAGTACATTCATATTTTGCAGCCATGA12.7027027027027026No Hit
CTCTTGAACCCCGTAACTTCTGAAATGTAAAAGCTCGAGGTCACAGGCTA12.7027027027027026No Hit
GCTTCGAGTGCCGGAATCGCGGGGTTCGACACCGTGAGGTCGAGCCAGGG12.7027027027027026No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12.7027027027027026No Hit
GAGTTCAGCATTTCTGCCATCTTGCTATTTTCATTTTCCCTGTGGAAGAT12.7027027027027026No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACACAGAGTCACGCACT12.7027027027027026No Hit
AAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGT12.7027027027027026No Hit
TCTCAGCTCACTGCAAGCTCCGCCTCCTGGGTTCACACCATTATCCTGCC12.7027027027027026No Hit
CCCTCTTACGGGGAAAATTCCTTACTGGAGCTAGTTTGGCTTGAGTGAAC12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)