FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAATTTCGGGCTGTGGTTCCGCCACAAAGGCGCCGACATGTTCCAGG23.9215686274509802No Hit
ACTCCGAAAGGGTTGGAAGGTGGGCCGATGCTAAAAGCATCGCCATAAAA23.9215686274509802No Hit
GAGGAGGCGTTCGAGCGGGAGCGGAGCGGCGCAAGCGGTGCTTGGACCAA23.9215686274509802No Hit
CTGGTACATATCGACCGGCCCGGCGTGGTGCCGGACGGACTCACGGTCGA23.9215686274509802No Hit
CGACGACGCCGCGCTGCTGCCGCTGACCCGGCTGTGCACGGCCTGGGCCG23.9215686274509802No Hit
GCTGATCGCCGGGCGTTGCCCCGTCCATCAGACCTTGACCTCGGAGGTCC23.9215686274509802No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATCAGCAGATCCGTGACGGCCTCCGTGCCGGCGGTGCGGCGGTGCGCG11.9607843137254901No Hit
CTACAGCCAATGCCTTGGCGACGCACTTCTAGCCATTCGGCTTGACTTAA11.9607843137254901No Hit
GGTGTGGTCCAGGCCATCAGCGGAATGGCGGCTGTCTCTTATACACATCT11.9607843137254901No Hit
AGGAATAGGTACCAAGAAAAAAAACGAACCTTTGGGTTCCAGAGCTGTAC11.9607843137254901No Hit
GAGCAGAGTTATATGTGTTTTCATAAAAAAATCTGAGCAGCAACCCATTA11.9607843137254901No Hit
GGGTAGGAGGAGTGCTGCGGGGGGTTGTGGGAGTCCGCGAGGGAGATGTG11.9607843137254901No Hit
CACCGCGCCTGGCCTTGGATTTGCATCGTTTAAAGACACCTCTGGCTGCT11.9607843137254901No Hit
GCTCACGGCCGCGGAGGCCGCGTGCACCGAGGCCGCGGACGGCCTCGCCG11.9607843137254901No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCT11.9607843137254901No Hit
GTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTT11.9607843137254901No Hit
CCCTGGCACGTGGTGGAGGCAGACGACAAGCGCACGGCGCGGCTGAATCT11.9607843137254901No Hit
CGACGACGCCGCGCTGCTGCCGCTGACCCGGCGGTGCACGGCCTGGGCCG11.9607843137254901No Hit
CCCTGCAGGCGCGCATCCCGGCGCTGGATGCACACCTTGACGTGCCGTTG11.9607843137254901No Hit
GATCTACCTCTTCCCACTGATGTACCTGTTCAGTGGTGTGAGCTTCCCGA11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCTGCACGCAGGTGGCGATCGTGATCCAGAACGGACCGATCACCGACC11.9607843137254901No Hit
AGCCGCGGCCCCTACTTCGACCAGGGCGCGCGCTGGGGCCAGCGCATGGG11.9607843137254901No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA11.9607843137254901No Hit
ACGCTCGTGGTGTGGTGCGGAAGGCGAGCAAGATTGCGCGGCGCATTCAC11.9607843137254901No Hit
GGTTCACCCGGTCGATCGCCTCGTCCGCCAGGTCGTTGCAGGTCACAGAG11.9607843137254901No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGCATAACCTCGGCGAAGCCCACGGTCCCCGCCGCTGGCAGGACTAGCAT11.9607843137254901No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTCCAGTCGCTATCGGAGTAGCCGCGCTGGTGCCACACGACTACAACC11.9607843137254901No Hit
GGCGACCGTGTTCGCGACCGAATCGACCCCCCAATGCGTGTTCCAGCCGC11.9607843137254901No Hit
TCCTTGTACAGCGCCTCGTACTGGCGCGAGACCGCCCCCAGCGTCGCCTT11.9607843137254901No Hit
GTACAAAAGCGGGACCTGCGGCGCATGCCACGCGGTGCCTTTGATACTGG11.9607843137254901No Hit
GATCCGGTCGGCCTGGCAGTCCTCGCAGCCGCGCGATACCGACGCTGATC11.9607843137254901No Hit
CTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGC11.9607843137254901No Hit
GACTGATCCAGTGGGCCGACAGGCCCGTCCCCTCGGTACCCATGAGCAGA11.9607843137254901No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGG11.9607843137254901No Hit
GTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCG11.9607843137254901No Hit
GGAACGGATCAAGGACAACATGGCCGGCCTCGGGGTCGACGCCCTGGTGG11.9607843137254901No Hit
ACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATC11.9607843137254901No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA11.9607843137254901No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)