FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT24.444444444444445No Hit
CATTTTATCTGTTCTAGAAATGACAGAAGATGGGTCCCTCTGGGATTCAC24.444444444444445No Hit
CCTTGGGAAAGCTTCAAGTTTCTCATCATTAAGTATAATGCTAGCTGTAA24.444444444444445No Hit
ACATGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGT24.444444444444445Clontech Universal Primer Mix Long (95% over 23bp)
GTGATGATGAGCATTTTTTCATGTGTTTTTTGGCTGAATAAATGTCTTCT24.444444444444445No Hit
GTGCTACCATGCCTGGCCAATTTAAAAAAATATTTTGTAGATACAGGGGC24.444444444444445No Hit
TCGGTGGTCTGACCGCGTTGTTCGCCTTCGCTGAAGCTCCAGAACTGTGC12.2222222222222223No Hit
TTCCAGAACTTTGTCATCACCCAAACTGAACTTCCTTTTCTCCAGCCCCT12.2222222222222223No Hit
TTCCATTCCTTTCCATTAGAAGATGATTCCATTCGAGACCATTCGATGAT12.2222222222222223No Hit
AGATTATAATTTTTAGTACAAATTAGAGGTTGAAGGTATTTTCTTCTTCT12.2222222222222223No Hit
TTGGCGGTCTTGCCTTCGTAGAACTTCAGCAGTTCCTCGCGGGTGACTTC12.2222222222222223No Hit
GCCATGCGTGTATATAATTTGTTATATACACTCAGTATAGACGAGAAGTT12.2222222222222223No Hit
CAATGGGAATCACAAACAATAAACTTACCAACGCACTAACATACAATCGG12.2222222222222223No Hit
GGATGAAAGATTAAGTGGTAAATGCCCTCTTCCAAGGCAACAGGTAGCCC12.2222222222222223No Hit
GAACCATGAGAGGACTTCGAGAAAAGTTTCTGTTTTTATGTATGATCTGT12.2222222222222223No Hit
GCACCAGTTCGGCCCTGGCGGTGGGGCCGGCCGCGGTCACGTCCCTGATG12.2222222222222223No Hit
ACATGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATT12.2222222222222223Clontech Universal Primer Mix Long (95% over 23bp)
CTTTTCTTTTCATTCAGCGGTTTGGAAACACTGTTTGTAAAGTCTGCACG12.2222222222222223No Hit
GGCTTCGCGCACTTCGTGTCGCTGCGCCTACCCCCCTCGCCGGGGGCGAC12.2222222222222223No Hit
GCAAAGATTGCCATGACAAGTGAGCTGGGCCAGCTTGCTAGATAAATTCA12.2222222222222223No Hit
CTGAAGGCACTGTTCCACTGAATCATCACACCAGCATTAAGTGGAACAGT12.2222222222222223No Hit
GATACTTGAAGAGATATCTGAGGAAACTAGAGTCCTCTTGGAAGCTGGAG12.2222222222222223No Hit
CCGGCAATGGAGCGCTGCAGTCGGCATTCCGCTCGGCGCGAGCATCGCGA12.2222222222222223No Hit
TGATAGAGTGGACAGAGCGAAGGATGGTGCATGAGCACACAGGGAAGATA12.2222222222222223No Hit
GAATCATCCAATGGATTTGAGTGGAATCATCATTGAATGGAATCGAATGG12.2222222222222223No Hit
ATTTTGGATTTTTTTTTAACGAATAAGTTAATTAGCAGCAGACAAAACCT12.2222222222222223No Hit
AAATTAATGGGCTATTCTACTTTTTTTGCATTCTTTGAAATCTGAAGATT12.2222222222222223No Hit
AGGTTAAACTCCTTGAGTTGAACACACACATCACAAAGTAGTTTCTGTGA12.2222222222222223No Hit
CCGCCCGCAAGACCGAGGCAGGGGAAGAGGGCGCCCGCCAGCTCATCGAC12.2222222222222223No Hit
CCACATAGATCTTCTCGTGCGAATTGACCAGGATGTCGGAGACCGAAGGG12.2222222222222223No Hit
TTATTACACTTTAAGTTCTGAGATACATGTGCAGAATGTGCAGGTTTGTT12.2222222222222223No Hit
ATAGTGATGTGGATAATGAAATCCAGGCTGAGGTGGTCTCAGATGGAGAT12.2222222222222223No Hit
GCTCGAAGAGCAAGTCGGCCAGCCGCTGTTCCGGCGCAAAGGACGCGGCG12.2222222222222223No Hit
GGGCAAGCTTAATCGCGTGAATCAGGAGTAAAAAAATGACAACCCAGACT12.2222222222222223No Hit
ATGTAAATGAGTTCTAAATCCAATGACATCCTTTTAAGAGAAACACAGGA12.2222222222222223No Hit
CTATAAGGGTCAAATGAGAGAGTTTTCCCATTCAAGCAAATTGGGGCTAT12.2222222222222223No Hit
GAGGAAGAGGATGGAGATGAAGATGAGGAAGCTGAGTCAGCTACGGGCAA12.2222222222222223No Hit
CTATAAAAACTCGACAGAATCTTTCTCAGAAACTGCTCTGGGATGTGTGC12.2222222222222223No Hit
GTTTATGGTGGGCACGCCCCTGGGGGTATTGGCCGCCGCCCTGGCCTGGC12.2222222222222223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)