FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATATTATTCTTCAGATACAACCATAAAGGCAAATAAGCCATAAAGAC31.6129032258064515No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA31.6129032258064515No Hit
CCCTTAAAGGAACCAATGAGTCCCTGGAACGCCAGATGCGTGAAATGGAA31.6129032258064515No Hit
CTTCGTTAGTAATTTCTCATCCTCATGTACAGCCTACTGCGGTCAATGCT21.0752688172043012No Hit
GAATTGACTCATGATTTGGCTCTCTGTCTGTTATTGGTGTATAGGAATGC21.0752688172043012No Hit
GCAGAATATTTTGTAGTATCTCCAGAGGGAAATAATTATGGCCTAAACTG21.0752688172043012No Hit
GCCCGCAGCCCGTCAGCGGCCTGAAGCTCGAGCCGACACCGGCGGAAGCA21.0752688172043012No Hit
GTGCCGGCCGATGGGAGCGGCGCATGAGCTCGATCAGGAAGCTTCGCACC21.0752688172043012No Hit
GCGCTGTAAGTCTCTGATTGGATTTTGCCTTGAATTTTTTCTGGTCAAGA21.0752688172043012No Hit
GTTTGAGACCAGCCTGGCCAACACGGTGAAACCCTGACTTTACTAAAAAT21.0752688172043012No Hit
GCACAAGCTCACGCTGATGCGCCGCAAGGACGCCTCCACCAACAGCTTCC21.0752688172043012No Hit
GGATAAGGTTTGAGCTGATATCTAAAATACCACCTGCTCCTGAAGGTGAT21.0752688172043012No Hit
GAACTCGCGCGGCAACGAAGTGCACCTGATGGTGGTGGACTACCAGATGG21.0752688172043012No Hit
GCTGCAGGCCAAGTCTTCTGGAGTGGCTGTCAGCAACATCTCATCAGTGC21.0752688172043012No Hit
CACGTACCACATTTTCTTTATGCAATTCACCATCGAAGGCACCCAGGTTG21.0752688172043012No Hit
GAATCTGGGTGCTCCTGTACTGGGAGCATATATATTTAGGATAGTTAGCT21.0752688172043012No Hit
ATGCCCGGCTGCACCTGCCCGAGCGGCGTGGAGGGGTCGCAGCACCAGGC21.0752688172043012No Hit
CTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGC21.0752688172043012No Hit
CACTGTTATAGCCAAAATCGGCAATGACACTAAAGAAATCCTCTGTGCTT21.0752688172043012No Hit
CAAGACTACACAGCCAGGTGCAAGCATTGGCAATGCTGATGGGGAAGAGC21.0752688172043012No Hit
AAAATGAAGTTAGCAGAACAGACGGTTGAAAATATTATTAAAATGAGTTT21.0752688172043012No Hit
GTCATTATAAGAGGAGTGAGGAAGCGATATAAAAGAAGAGAAGGAAGCCA21.0752688172043012No Hit
GTTTCTGTTGATCTTTTTGTTTAACATTCTGGGAGATTTACTGAATTTCT21.0752688172043012No Hit
CCCTTTTACTCTGTCATTTAAACAGACTTTGAAACTAGATATAAAGTGTA21.0752688172043012No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG21.0752688172043012No Hit
CTTGCCTACTGATCAGTTACACAACCAATGCATTTCCTGGAGAATATATC21.0752688172043012No Hit
ACCACAAGCAGTGCTTCTCCAATGCCCAACAGTTATGATGCCCTGGAAGG10.5376344086021506No Hit
GCCCAGAGGGGAGCTGGAGCCAAGAAGTTGCAGCAACTGCAGGAAACCCT10.5376344086021506No Hit
ACTAAACTGGAGGCTGAATACCGTGGAGGTCCACATGCAGCTTCCCTGGA10.5376344086021506No Hit
GAACTCGCGCGGCAACGAAGTGCACCTGATGGTGGTGGACTACCAGATCG10.5376344086021506No Hit
GTAGGATTTATTCAGGATCTTCCGACGACCTTGATTGCTGCATTCCGCTC10.5376344086021506No Hit
GAGCCAAGATCGTGACACTGCACTCCAGCCTGGGTGACACAGTGAGATTT10.5376344086021506No Hit
AGCTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGA10.5376344086021506No Hit
GATAGTCTCTGAAGCTTTGCTTAGCCATTAAAACATTTTAATTTTTTTTT10.5376344086021506No Hit
GTATAGCTGAGCTACAGGCTGGACCAACTCGTGAGCAGCAGCAAAGAGCG10.5376344086021506No Hit
CACCAATGCTGGTCTCAAACTTCTGGCCTCAAGAGATCCTCCTGCCTCAG10.5376344086021506No Hit
ATCATCGAATGGACTCAAATGGAATTATCCTCAAATGGAATCGAATGGAA10.5376344086021506No Hit
CTGTTGTTACTGTCCATTACCAAATTTGTTAACTGATCGATATATTTTTT10.5376344086021506No Hit
ATCAACGCAGAGTACATGGGGGAAAACACTTTTTTAAATAAAAAAAAAAA10.5376344086021506No Hit
AGTGACGGCTGGCACTGCAACTGCTAGTCTGGGGGCTTGCCCTGGAACTG10.5376344086021506No Hit
CTTCTCCAAGTGGTAAACTTAATCCATAATCAATTCCTAAGGAACCCCTG10.5376344086021506No Hit
TCCTCCCACCAGGCTACACATCTGCCAGTGAGCTATACTGTTGCTTGTAG10.5376344086021506No Hit
CATTAAGCTAGGTCTTGGACACATAATATAAATAAATTAGACATGTTTGC10.5376344086021506No Hit
CTTTCAGGCTGACTTGTCGGCTACACCTTTCTCAAACCCCAATTCGCCAT10.5376344086021506No Hit
ATTCAACAGGTTACAGGAGGAGCTATGAATATTTATGAAGATCCTGACAC10.5376344086021506No Hit
GGTCTAATATCCAGAATCTATAACGAACTTAAACAAATTTACAAGGAAAA10.5376344086021506No Hit
AATGCATATTTTTGAGTTTATACCTAAGTTGTTTTTCAGTTACAACTTTG10.5376344086021506No Hit
GCATCCGCCTCTTTCACGAAGCTGTCGACATCGCGGCCCTTGACGTCCAT10.5376344086021506No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATACCTTTCTATCGCGTA10.5376344086021506No Hit
AATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCT10.5376344086021506No Hit
TGATAGGGAATACGGAAAAAAGAGGAAAAGAGAAAATAGTTATCCAATAG10.5376344086021506No Hit
AGACGTTAGTCACGAAGCACCGGGGCAAGCGTCGCAAAATATGTTTGGCG10.5376344086021506No Hit
TCCTGGAGGAACACCTGGCCACTGCAGTTTAGGCATTTTGAAGTTCTTGT10.5376344086021506No Hit
ATGGAAAAGCCAGAACTCTTGACTTCCTACTTGAGATTTGATAGCTTTAG10.5376344086021506No Hit
AAACTTTATTTGCCCTAATAGATAATCTGGTGAAGGCTGTGGACCAAAAT10.5376344086021506No Hit
GCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCAAAAAAAAAA10.5376344086021506Illumina Single End PCR Primer 1 (97% over 40bp)
ATTCCATTTGATGATGATTTCAATCAATTTTATTCGATGATTCCATTCGA10.5376344086021506No Hit
ATGCCGATGCGGGGTTGCTGCTGGGTCATGGGGTACTCCGGAAGAGGTTG10.5376344086021506No Hit
GAGCCCATCCGATCGCGCGGCGGGGACTCCGGCCGCGGCCCGGCGCAGCC10.5376344086021506No Hit
GGTCTCGAATGGAATCATCTTCAAATGGAATGGAATGGAATCATCCAATG10.5376344086021506No Hit
GTAATGTATAAGCACTGAATTGCTGTGAGACATGTCAGCCACTCTAGAGT10.5376344086021506No Hit
GCTAACATGGTGAGCGGGCTTAGAGAAGCAACTCGGCGGGGCAACGTGGC10.5376344086021506No Hit
GGACCTGGGCCTGGTGCTCGACGTGGCGCGCGCGAGCAAGTTTCCGCTGC10.5376344086021506No Hit
GATTATCAAGGTCATGTTCAAAATTAAAATGAGCTGTGGGACAATAAGCT10.5376344086021506No Hit
GATGATGACCCCGCCCCTTTCCTGGTGTTGTACCAACATGGCAAGAGTGT10.5376344086021506No Hit
CCCCACATGGTCATGGTCATGATGGAGTCACCATGTAATGCATGCACACA10.5376344086021506No Hit
GTATGGTTACTTGAAGTATGGTTTCTACATGATGTGTTTTGCTTTTGCAG10.5376344086021506No Hit
TCCCTATTGTGTGGCTGATCACTAGATCTTACTGCTTCTATTTTTGCACC10.5376344086021506No Hit
ATAACCAACAGAATGGGAGAAAATATTTGCAAACTATGCATCTGACAAAG10.5376344086021506No Hit
GTTAAAAGGCTGTCATTTCCCCAGTTCCTGTGATTTTGTCAAAGATCAGT10.5376344086021506No Hit
GAGTAGGCGAATGCTATGAAGCCCAGGCAGCAGGGGTTCATGAAGAGGGT10.5376344086021506No Hit
ATTACAATCTACGTGGTCCAGCGGACTCAGCAGCAGAGCATGTGGCTCTC10.5376344086021506No Hit
GTCACCCCACAGTGCAGTCCCATAGCTAAGGGTTCTGCCTTCTTTTTTCC10.5376344086021506No Hit
TCCTTGAAGAGGTCCTTCACTTCCCTTGTAAGTTGGATTCCTAGGTATTT10.5376344086021506No Hit
GTTCAAGGAAGCCATGTCGCCCGAGTTCAAGGCCTACCAGCAACAGGTAG10.5376344086021506No Hit
CTCTGTGATAGTTAACCAAATCACATTGGCAACATGCTCTCTTGTTTAAA10.5376344086021506No Hit
CATATTAACCAACATTTGCTGTTTTCTATTTTCTTCTTTACTTCTTTTTA10.5376344086021506No Hit
TCTTGATCCCTTTCCTTGTTGTCGCTGCACTCTTTTGGTTTCAATGCAAC10.5376344086021506No Hit
GCCCTGCCCAGGCATAGTTCACCATCTTTCGGGTCCTAAAACGTGCGCTC10.5376344086021506No Hit
GAGATGGGGTTTCGCCATGTTGGTCAGGCTGGTCTCAAACCCCTGACCTC10.5376344086021506No Hit
AACCTAGGGGAAGGGAGAAATTAAGAAAGTGATGGAAGATGGAGACCCCT10.5376344086021506No Hit
GCCCTGGACCAGCATGTGGCCCAGGTTTGCCAGGACTGGCAGGCCCAGGG10.5376344086021506No Hit
CACGACGACGCGTCCGTACCAGACCTCCACCGACGGTGACTACAAGCTGG10.5376344086021506No Hit
CTACTGTCAGCATTAGAAAGAGATCATGTAGACAGAAAATGAACAAAGAA10.5376344086021506No Hit
GTGCAGGTGCTCTTCGCGGCCAACGGCCGCCAGCAGTGGCAGTTGGCGGT10.5376344086021506No Hit
TTTCCATATTCTTTCTTACCTGTAGCCTTAAGTGTCTCACAATTATTATC10.5376344086021506No Hit
CAGTCTCGCTCTGTCGCCCAAGCTGGAGCGCAGTGGCACAATCTCTGCTC10.5376344086021506No Hit
GCAAGAACGGGCAGGGCAACTGCTACAAGAGCAACTCCAGCATGCACATC10.5376344086021506No Hit
GTGCGCACCTGCGAGACGATCATGCTCGGCGCGAGGCCACTGTCTCTTAT10.5376344086021506No Hit
GAATTGGTATGTGGGGAATAGACATAAACAAGGGAGAAATATGATGAAAT10.5376344086021506No Hit
GGGCATGAACCACCGCTCCCAGCCCCATTATTCTTATTTTTTAAGAGATG10.5376344086021506No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAAC10.5376344086021506No Hit
TCATGGAGCTTACATTCTGAAACACGGGTTCTAAAAATGTGGTCCCTGGA10.5376344086021506No Hit
ACGTGCCCCTGCTAAGTTCTCTTCAGTTTGTTCTTTGTGGCTCTGATCAA10.5376344086021506No Hit
GACTTACAAAGAGTGTATTTCCAAACTGCTCGATTAAAGAAAGGTTAAAC10.5376344086021506No Hit
CCACTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGC10.5376344086021506No Hit
ACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAAC10.5376344086021506No Hit
GTGGGAGTTCCAGGAAAAAAAACGGGGAAAGAAAAAAGATCAAAAATGTA10.5376344086021506No Hit
CCTATCGGCAACTGTGCTGCTTACAAGTACATGGCTATTCAATCGCTAGA10.5376344086021506No Hit
CATGTACAAGGTCCCTTCTTTTCTAATTTAAACATTTATGAGTAATAAAA10.5376344086021506No Hit
ATTTGGAGAAATGGAATCCTTTGATGATAAGACCTTGGCCGGCTGTTTCA10.5376344086021506No Hit
GCCTTGTGCCGAATTATACAAAATTGGACATCAGATTTATGCGGCCCTGT10.5376344086021506No Hit
AGCTTTAGCTTTGCCTTCTTTAAAAGGTGCCAGGAAGGTGTATTTAAGAA10.5376344086021506No Hit
CGGTGGTGGTCAGCGCCGGCAGGTTGACCGCCGTGCGCGCGCCCCATTCG10.5376344086021506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.5376344086021506No Hit
GGGTACCAACTCTTCTTTGTATATCTGATAGAATTCAGCTATGAATCCCT10.5376344086021506No Hit
GATTCAAAATATTTAAAAGATCTCATATTAAAACAAATCACATAAAGAAT10.5376344086021506No Hit
CCATGGCACCCCACTTCTGGCTGCCCTGGACTACCAGGAGCATTTGTGTC10.5376344086021506No Hit
ACCTGGTAACGCAACCTGCCTCCTCAATGTACCTGTTTTGCCTCTGAACC10.5376344086021506No Hit
GCATTGCACTGCCACTGCTCATCATGACGTGCAGACAGCACTCATATACC10.5376344086021506No Hit
CCATATAACACTTAGAATGAAGGTAGATGTAGAATAGGTAAAATTCTACA10.5376344086021506No Hit
GTGCCTCAGGATCGCTCCTTGTTCCTGGTCGAGCAGCCCGAGCGACGTCT10.5376344086021506No Hit
GGGTCCCCAGGTCCACTCGGTCAGTCTGTGACTGGGCCTTCACATTACGT10.5376344086021506No Hit
GCCCTGCCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTC10.5376344086021506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.5376344086021506No Hit
CTTCAATAATAAAGTTTAAAATCCTCAGTATGGACTAAAAGATCTGGCAA10.5376344086021506No Hit
GAACAAAGCTGGATGGAGAATGACTTTGACGAGCTGAGAGAAGAAGGCTT10.5376344086021506No Hit
GAGCCAAGATCATGCCACTGCACTCCAGCCTGGGTAACAAAGCAAGATTC10.5376344086021506No Hit
GGGCCTGGTGGAGTTCAGGGCAGCAGCTCTTCATCTACAGGATGTTCTAG10.5376344086021506No Hit
CATGCAATCAGGTGACTACGCATCGGACATGTGAGTCACGACTCGGAGCC10.5376344086021506No Hit
GTATCAGAACGTTGCTAGGACATTGTCTACGATGACGTCATGCCTCTACA10.5376344086021506No Hit
GTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTATTGGCA10.5376344086021506No Hit
AGCAACGTGAGTCTCGTCACGTCGATCGGGTGACGATACGCGATGGTGAC10.5376344086021506No Hit
GTACATGGGTGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCAGGCCAA10.5376344086021506No Hit
CCTACATTGTGGGCATTTGCAGCTATGGCTGGTGCGCACAACAGCTTGTC10.5376344086021506No Hit
CTGCTCGCGAAACAGCGGCACGACCGAGCCGCTCGACCCCAGGACGTTAC10.5376344086021506No Hit
ACTTTGGGAGGCTGAGGCGGGCGGATCACCTGAGGTCGGGAGTTCGAGAC10.5376344086021506No Hit
CACCTCGGCAAGCGGCGCAGGCCGCGCGAGGTGATAGCCCTGCACCAGCC10.5376344086021506No Hit
ACTTAATATCTAAAACTGAATAATGAGAAATGAGAATATTTGGTAATAGG10.5376344086021506No Hit
GCGTGCAATGAGGTCATCAAGCACAGAACAAATGTCTATACATGCCACAT10.5376344086021506No Hit
GTCGTTGGCCGTGCGGTCGAGGTGGATCGCGAGCTGCGGCAGGCGCAGGA10.5376344086021506No Hit
GCCAAGCGCATGGCGGGCACGGGCGTGACGGTGAACGCCATTCTTCCCGG10.5376344086021506No Hit
GATACACATAAAGACATGGAGACGTCATAGTAAAAATTTCAAAAGCCAGT10.5376344086021506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC10.5376344086021506No Hit
ACCTCATTGTCAGCCTCCTGCTCCCCATTTTCCTCATTAGCATTCCCGTT10.5376344086021506No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATACCTTTCTATCTCGTA10.5376344086021506No Hit
CGGTCTTAGCACGCTCTTCTGACAACCACTGGTTGTGTGCTGGCTTACCT10.5376344086021506No Hit
TCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCG10.5376344086021506No Hit
GAGCAGGAACCCAGAGTCCTGTGCAGTCACAGGATGACGCAGGGAGGACG10.5376344086021506No Hit
GACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCTTGGAGAC10.5376344086021506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTATTTTTCAATG10.5376344086021506No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAATGCTTTGAAACCACTG10.5376344086021506No Hit
CCCTCCACGCGCCCCGCGCGCGCGGGCGGGCGCGTGCCCCGCCGCGCGCC10.5376344086021506No Hit
CCTGTCACCAGCGTCGCCGAAATGATGGTCCCGGCCATGACCTGGTAAGG10.5376344086021506No Hit
ATATTACCCCAACCCCCCAGTGGGGTACAGAAGCGTATATACTCTTTCTC10.5376344086021506No Hit
GCCTTGTGCCGAATTATACAAAATTGGACATCAGATTTATGCGTCCCTGT10.5376344086021506No Hit
GTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCA10.5376344086021506No Hit
CAACAGAGCGAGACTCCGTCTCAAAAAGAAAAAAAAAGATAGAATGTAAA10.5376344086021506No Hit
GTATCAAGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.5376344086021506Clontech SMART CDS Primer II A (100% over 26bp)
GTATTGCAGAAGCCGGTCGATCATGGTTTTCTTCGACCCGAAGTTGTAGT10.5376344086021506No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.5376344086021506Clontech Universal Primer Mix Long (95% over 21bp)
TCACATGGTCATCCCTCTGTGCATGCAGGCTCCTCATGTTTCTATGATGA10.5376344086021506No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC10.5376344086021506No Hit
GTTTGGCTGTGTATGGAAATGATACAGGAAAATAGGAGAAAATTTAATGT10.5376344086021506No Hit
GAACTATGCAAAGCTACAGAGGACATCACAACGGAAGTTATTAATGTGAC10.5376344086021506No Hit
ATACACCACCAGACTGGCTTGTGCTCTGGCAACTGCAGGGTGCTCTCGCG10.5376344086021506No Hit
GTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTC10.5376344086021506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)