FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGTATGGGCTTCTGCGCACCAAAGAAAATTCCATCATTTTGCTACA31.6129032258064515No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT31.6129032258064515No Hit
CTTGGGCAGCGCGGCCGCCTTCTGCGGCGGCTGGCTGAAGTTCGTCAGGA31.6129032258064515No Hit
CCTTTTTGAGTGGGTATCAACCAGAGGGAGTGAATCCAGATTAGTTTCCC31.6129032258064515No Hit
CGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGA21.0752688172043012No Hit
CACCACACCAGCGCGCCGCAGAAGAACATCGGCAGATCGACCGCCTTCGC21.0752688172043012No Hit
GTATTCCATGGTGTGTATGTGCCACATGTTTTTAATCCAGTCTATCATAC21.0752688172043012No Hit
TCTTGGGGTCGCGGTACAGGCCGATGTGGCCCACGCGCGCGCCGGGCACC21.0752688172043012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21.0752688172043012No Hit
GCACCGCAGATAGCCTCAGTGACACAGTGGCTCTGCAACTCAAAGTGCTG21.0752688172043012No Hit
TTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAA21.0752688172043012No Hit
TGTTCACATCCGTTGTTTCATTTGAGCTTCTCAGTAGCCCGTCTCTGTGA21.0752688172043012No Hit
CCCTGAGGAGGACCTGTCCAAACTCTTCAAATCACCACAGCCGCCTGCCA21.0752688172043012No Hit
GCCTGTTTGTTTTATTTTGTTTGTTTTTGAGACAGGTTCTCCTTCTGTCA21.0752688172043012No Hit
CCAAATGGAACTCTTATCCCTTAAGCCAGGTCATTCAGTTGTTGTAGCCT21.0752688172043012No Hit
GGGTACAGCTTGCTCAGCTGAAGTTGATGGCAGAACAGGCCTGGTCTCTG21.0752688172043012No Hit
GTCTTGTAACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTC21.0752688172043012No Hit
AATTCATCCGGCGCATCACGACCTGGGTGGCGCTCGAAGACCGGGGCGGG21.0752688172043012No Hit
CTGTCCATGAGTTAAGGCAATCCATGACTCATTTTTTTAGTCACCCTGAA21.0752688172043012No Hit
CTGTTGCCCAGGGTGGAGTGCAGTGTCACAATCTCGGCTCGGCTCGGCTC21.0752688172043012No Hit
ATTCAGGACGACAAGGCTGATGCTGTTGATGAAGATGCACTGCAGAGTTT21.0752688172043012No Hit
CAACTAATGTACTAAAGCCCAGCAGTACACGATTTTTAAAAAATTAATTT21.0752688172043012No Hit
GCCGGGGGCATTCGTATTGCGCCACTAGAGGTGAAATTCTTGGACCGGCG21.0752688172043012No Hit
CCAATTAAAAGACACAGACTGGCAAATTGGATAAAGGGTCAAGACCCATT21.0752688172043012No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGATATCATCTGCTT21.0752688172043012No Hit
GAGAAAATATGTGCAAACTCTGCATCCTACAAAGGTCTAATATCTAGAAT21.0752688172043012No Hit
GTGGTGCCGCACCTCAGGATACCACTTTGCACGGACATTTTCAAATGATG21.0752688172043012No Hit
CCTTAAAGAAGGAAGGGGATGGAATGCAACAAGAGCTTGTTTCTTCTTCA21.0752688172043012No Hit
ATCAGTGATCTTCACCACCATTCTACTGTCCACTACGCAGTTGGTAGATT21.0752688172043012No Hit
GTCACACTCTCCCTTAAGCTTTTCATTACCATCAGCAATGCCAATAACTT21.0752688172043012No Hit
GTTTCTTGGGTGGGAAAATTCTGAGACATGTGTTCTGCAATATGCCCCAG21.0752688172043012No Hit
GCGCGCGGTAGCGTTCGAGCGAGGCCATGTCCGATTCGCTGCGCGCGATC21.0752688172043012No Hit
CCGCCGCTCCTGCGCCTGCCGCAGCTCGCGATCCACCTCGACCGCACGGC10.5376344086021506No Hit
GTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCCCG10.5376344086021506No Hit
CATTAGATTCCATTTGATGATGATTCCATTCGATTCCATTTGGTGATGAT10.5376344086021506No Hit
CATCGCATAGAATCGAATGGAATCATCATCGAATGGAATCGAATAGAATT10.5376344086021506No Hit
GTCTTCACTGGCCACCCTATGCTTACCCACAGCACCTATTAATATCAAAT10.5376344086021506No Hit
ACGCTATTAAGTTGAGGCTGAGAAGTGCCAGAAAAATGTCCTTTTTTTCT10.5376344086021506No Hit
CAAAAGATAGACTGGTTACTAATTCAATGCTACTTGGAGATTTTGTTCTT10.5376344086021506No Hit
CGGTAAGATGCGCCGCCCGGGCAAAGTCAGAAGGGCGAATGGGTCAACTG10.5376344086021506No Hit
GTGGCTGTGCTGGGGGCGCCCCACAACCCTGCTCCCCCGACGTCCACCGT10.5376344086021506No Hit
GCCTCGAGCCTGCTGCGTCGAGGTCCCTTACACACGGCACAGATCTGTAG10.5376344086021506No Hit
ACTTGGTGGTGGCCCCTCTCAAGTGCCCTTGGTGAGAGCCCTAGTGGTGG10.5376344086021506No Hit
GTATATTGAGAAAACCCCATCATCTCAGCCCAAAATCTCCTTAAGCTGAT10.5376344086021506No Hit
GTGTAGAAACAGCAAAAATGTCATAAAAATTCTTATATTTAAAACAAAAA10.5376344086021506No Hit
CGCTGCCCCGTGTCGCCCGACACGCCCGTCGCGCTGGCCTCGCCCGGCAA10.5376344086021506No Hit
CTGCCAAATCTATACACCTACTTGCATCTGTACCCATACTCTCCAGATTC10.5376344086021506No Hit
GTCTCACTCTGTCACCCAGGCTGCAGTGCAGTGGCCTGATCTCAGCTCAC10.5376344086021506No Hit
GACCGGGCCGCACCGGCAAAACCCAACGCAAACTCTTCACCGCCACGATG10.5376344086021506No Hit
GCACAAAGGGACGGAAGAGGACCTTGCTTCCAGACTGTCTGTGGTTTGTG10.5376344086021506No Hit
GTGCTGCTGCTCGTGCCCGCTACCGGCTTCCTCGGCGGCCTGCTGCTGCT10.5376344086021506No Hit
GTAGAGCATGTGACTCTTGATCTCGGGGTTGTGAGTTTGATCCCATGTTA10.5376344086021506No Hit
ATTGAGAGAGCTGGCATTTAAACTAGAATGCAAAGGAAAGCAAGGGCTAA10.5376344086021506No Hit
CAGCACATGCAGATGGCGCAGCTCATATAGTCGCATGCCTAGCTCCTCTG10.5376344086021506No Hit
ACATTAGAAAAAGCTCCAAAAATTAAATTCTGGCCCTCAAACCCCACAAC10.5376344086021506No Hit
GCCACACACGCTGCAGGTGCTGACCGGCGCGCCCGCGCGCCAGACCTGCA10.5376344086021506No Hit
CATTAAGATAACAGAAAACTGCCTATAAGAAATTTCAGGATCACGTTTAG10.5376344086021506No Hit
ACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAACCTCCCGTGTA10.5376344086021506No Hit
CCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCG10.5376344086021506No Hit
GGACATGCAAGGGACGCCAGCAAGCAGAAATCGTAGGCATAGTTTGTCCG10.5376344086021506No Hit
GCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGT10.5376344086021506No Hit
CTTACAGCCTCTGTAAAGTTGCCATAACTATAGCTTTGATTGTACAAGAC10.5376344086021506No Hit
ATATCGCCCAGGCTGGTCTCAAACTCCTGGGCTCAAGTGATCCTCTTGCC10.5376344086021506No Hit
GCTAGCAGTGAGCCAACATCACGCCATTGCACTCCAGCTTGGGCTAGAAG10.5376344086021506No Hit
TTCTATGACGATTCCATTCATTTCCATCTGATGATGATTCCATTCCATTC10.5376344086021506No Hit
GAAGAGGTCGCGCTCGACACCCACGCCACGCGCGGCCGGCTGAGCGCGCT10.5376344086021506No Hit
CCATAGGTGCAGTCGAACACTTACAGTGCCTTGACTTTGAAATTGATGCG10.5376344086021506No Hit
CCGGTGATCCGATGCAGGGGATTAAAAATAAAGCGAGAGTTCAGGGACTC10.5376344086021506No Hit
AACAAAGTAAGCACATTTTCTCTTGCCGGGCAGGAGAATGTCAATGCCGT10.5376344086021506No Hit
GTTGAGTACCATCTCAACCTTACTTTTGAGATAAGTCCTTTAAAGAAGTA10.5376344086021506No Hit
GAGTCAGGATACCCCTCAAGTCTGCACTGTTAATCCCTGTCCAGATGGGC10.5376344086021506No Hit
GGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGA10.5376344086021506No Hit
GCTTAAAGAGTTATGTAGTTGGAAAAAGGAGAACATTACATATCTCTGAA10.5376344086021506No Hit
GTACAGGCTGTGTACCAGATGTTTGGCAATGCTACACGGTGCCAAATACC10.5376344086021506No Hit
GGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGT10.5376344086021506No Hit
TCAACCAGAAGTACAAGCAGATGGGTGAAGCGTCCGACACCCTCAAGCAG10.5376344086021506No Hit
GCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACC10.5376344086021506No Hit
CTACGCGTGCGTGCTGCCGCCGCTCGGCGAGACGCAGTCGGACAGCGGGC10.5376344086021506No Hit
GGCTCACTGAGCCTCAGAGGCAGAGCTTCCCAGGCTAGGGGAGTTAGGAG10.5376344086021506No Hit
AGTCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCACAAGGTCAGGAGG10.5376344086021506No Hit
GCTTCAGGCAATGGGGGAAGGGCAGGCACTGGTACAGACTGGAGGGTGCA10.5376344086021506No Hit
ATAAATAACTGTATAAAACACAAAATTTCAGTCATCAGAAGACAGATTGT10.5376344086021506No Hit
GAATATTACAATATTACAGGGACAAATGGGAATATACTAATTGGGTCAAG10.5376344086021506No Hit
TGGCCTCGCGCCGAGCATGATCGTCTCGCAGGTGCGCACCTGTCTCTTAT10.5376344086021506No Hit
CTTCAAGAGTTTCTGATGAGAAATCAGCTGTTAGTCTCATTGAGGATTCC10.5376344086021506No Hit
TTCTACAACTTTGAGAAAGTATCTCTTATGATTTTAACTTGGAGAGTGAT10.5376344086021506No Hit
GCCCATTTATTAAACTGCAAGCCAGACAGCTCTGCCAGAAAGATCTGCAT10.5376344086021506No Hit
GCCCTTGGTCGAGCACAATCCACTGGAGGGATTGCGCAACAATGTCATCG10.5376344086021506No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.5376344086021506Clontech SMART CDS Primer II A (100% over 26bp)
TCTGGCTGCAGTTCACCACCAGCAGGCCCGTGGCGTCCTTCTGGCATCCG10.5376344086021506No Hit
TCATGTGGGCGCTGCCCAGCACGGCTTCGGCTTCCTTGCCGGTGATGCCC10.5376344086021506No Hit
GCAAAGCGTTAAACCACAAGCAGGGGCAGCATCTACCTATGCGACAGCAA10.5376344086021506No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.5376344086021506No Hit
CTTTCCAACTCTGCTTTGTCTTCGGCCAATCTGGCTTTTTGCTCTTCAAT10.5376344086021506No Hit
ATCAACGCAGAGTACTTTTTCTTAAGTTTAGCTGCTTGTTATCATCTTTT10.5376344086021506No Hit
NTAAATAAAGATGTCTTACAACTCAACAACAACAACAAAAAAAAACCAAA10.5376344086021506No Hit
ACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGA10.5376344086021506No Hit
ATCTTTACTTAAATTCTATTCACACTTCAAAACCAACCTCAAATGATACT10.5376344086021506No Hit
GGTGAATTCAAGATCAACATAAGCAAGTTTTTCTGGAAATGACCAAAAAG10.5376344086021506No Hit
AGATCCTTGAGGTCGTGCAATCCGGAATCGGCGGCAGTGCGGTCTCCACA10.5376344086021506No Hit
GTAGGAGGCAGTGGGGAATAGTGTTTAGAGACTTGGATTGAATTCCCAGC10.5376344086021506No Hit
GCTTCAGCGCATTTTCAGCAAGCCATGTGGCAAATTGTTGCGCGTGTTCA10.5376344086021506No Hit
ATTACAATGTTAGGACCTGGAGAAGAAGGGTTTTCCTTAAAATTTTATAC10.5376344086021506No Hit
CTTTACATCACCAAAAGTGACGTTAAAAAGCAATTTGCTTCTTTCCTTGA10.5376344086021506No Hit
GCACCGTGGTTGGCAAGGGAATAGTCTATAGACAGCAGTACCAACATTAT10.5376344086021506No Hit
GTCTTAGGCAGGAAATGTGTAAAAGTTTATGTAAACACCCAGAATCCTAC10.5376344086021506No Hit
TAGCTAGACTAATAAAGAAGAAAAGAGAGAAGATTCAAATAAACACAATC10.5376344086021506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.5376344086021506No Hit
CTGCGCTGCCGCCGGCCGCCCCGCAGCTGGGTGAACCGCTGCCGGGCGAC10.5376344086021506No Hit
TTCCTGATATGACCAAGAAAGCTTCTTATCAAAAGAAGCACAATTGCCTT10.5376344086021506No Hit
CCCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGCCGCGGG10.5376344086021506No Hit
AGATAAAAATGCACGAATTGCCATTTGTGCTAAATAGGGGATTCCAAAAG10.5376344086021506No Hit
GGCCTTCTAGCTCTGTCTAATGCCTAGAATTTCTCTCCTGGGGCATCATT10.5376344086021506No Hit
GGAAGGAAGAGGGAAGAGGCAGCTGTGGAGAGGATGAGGTTGAGGGAGGT10.5376344086021506No Hit
GTGTAAAAGTGTTCCTATTTCTCCACATCCTCTTCAGCATCTGTTGTTTC10.5376344086021506No Hit
ATTTTGGGTCGCATCCCTGGGCTATGGATTCCTGTTAATCTGTCCCAGCC10.5376344086021506No Hit
CCTAAGGACTTGAGCAACTCTGGCCGCGAAGTCGGCCTGGCTCAGGGCCT10.5376344086021506No Hit
TCACATCGGCTCCCGAGGCTTCTGCATTCTTCACGTAGTTCTGGAGCCTT10.5376344086021506No Hit
ACCATAACCTGTTTTTGGCCATGCCTCGAGGGAGTTTCCATTCTTTCTGT10.5376344086021506No Hit
GCATGAAACACAAGACACAAAGTTGTAAATTGCGATGTCGCTTGACGTAG10.5376344086021506No Hit
ACAATAACCTTTCCGATGAGAGCCCTTTTGACAAAGCAGAGATGTCGGAG10.5376344086021506No Hit
ACCTGGAGCTGGCCAGTGCTGTGAAGGAGCAGTATCCGGGCATCGAGATC10.5376344086021506No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAAAAAAGCTTAC10.5376344086021506No Hit
CCTTCTTTATCCCGTTTTTCACTTTGCCAAAGCTTGTTCCCTCCCTCCAT10.5376344086021506No Hit
GATTCAAATTGGTCAGCCTTTTCATCAAACTCTTCATCCTCGTCGTCATG10.5376344086021506No Hit
AGTCTGAATATCATGTTCATTTCACGTGCGAGTATTTGCCGGCACGCGTG10.5376344086021506No Hit
AGTCGAAGTCGGCCAGCTGCTCTCAGATGGTCCGGAACGGAATCTCCGAG10.5376344086021506No Hit
AGGCGCGGGCACCGGTGCCCAGGCCGGCCAACTGATGGGCTTCTTTAAGG10.5376344086021506No Hit
CACTGAAGGCTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGA10.5376344086021506No Hit
ATTCTCAGTAACTTCTTTGTGTTGTGTGTATTCAACTCACAGAGTTGAAC10.5376344086021506No Hit
GTTTCCCTATTATTTATTTCTAGTTTTATTTCATTGTGGTCAGATAAGGT10.5376344086021506No Hit
CATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACT10.5376344086021506No Hit
GCGTTATTTCCCACATTATCTGGGAGTCAGGGTCGCGCCTAAACCCTAAA10.5376344086021506No Hit
AATAACCCACTGTTGAAGTGCTTTTAATCAGTCACCCCACACATGAAAAA10.5376344086021506No Hit
AGTTGACTCTTTAAGTTCGCTCACCAAGTCCGTTCGCCGTGTCGGCTCAC10.5376344086021506No Hit
GCCCAATAAACCCTGAGCAAAAACCACAAAGCTGGAAGCATCATATTACC10.5376344086021506No Hit
CTCGCGCATTGGTCGTGCTGGCCGCCCTTTCCAATCGGGATGGGACAAAC10.5376344086021506No Hit
GTCCTGGTGGACCTGCAGATGTTCAATGTGCGTGACAACCCAGGCAAGTA10.5376344086021506No Hit
AATCCAGAATATGTGAATAATTTACAGGTGGTCTTTGACTTACAATGGTT10.5376344086021506No Hit
TAACAGCTCTTTGTATTTATTCTATATGAAGTTTGTTGAGCTTCTTGGAT10.5376344086021506No Hit
GCTGTGAAGGAATTTGTTACTACTCCAGTATCTGTATTCTTCAATCTCAA10.5376344086021506No Hit
GGGGAAAAGTTTTGTTTAATTTGTGCCTGGAACATAATATGCCCTCAATA10.5376344086021506No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.5376344086021506Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5376344086021506No Hit
CTCTTTTGCAAATGGGCATATCCCAGTGAGGACCCTGAAAGTTATGCCCA10.5376344086021506No Hit
CTGTAGAGCTTCTCTCCCTGTTTTCCTTCCCCTTCACAGCTTTTGAATTC10.5376344086021506No Hit
GCCTGAGGATGGCCGCCAGGGACAGAGGCCTGAAGGCATCATCTGGGCAG10.5376344086021506No Hit
CATGGGCCCTGTGAGAGGCTCCACACTGCAGCCTTGACCAGGCGCGGCAC10.5376344086021506No Hit
AAAATAGGTGGCATTTATCCCGTGAGTAAAATCCTTCATTGGCTTCCTAT10.5376344086021506No Hit
GTCGAGCAGGTCGCGGCCCAGCGCCGAGAGCTGTCCGCTGCGCGGCGTGT10.5376344086021506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)