FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGACAACCGTCTTGATATTCTCAGACAGCGAGCTCATCACATCCTG22.5974025974025974No Hit
CCATAAGGTCACCCACATCCACCAGCAGGCGCGGGGCGGTGATGATGTTG22.5974025974025974No Hit
GTACTGGGATTACAGGTGTGAGCCATTGCACCCAGCTGAGTTCTAGTTTT22.5974025974025974No Hit
GTCTTTATGGAACCCATTCAGGGTGAGGCTGGGGTCGTCCCCTTGTCGGA22.5974025974025974No Hit
CTACCCGGTGGTGCGTCTGAGCTTTGCCGAAGGACGGCTGCAAACGGCCA22.5974025974025974No Hit
CTCCACCCCGCGCGAGTTTGCGGATAAGTACCTGCCGAACTTGGACGAGT22.5974025974025974No Hit
GTTTCGTTGGCCTCCACTGTGGGATCAGTGCGGTTAGTGGTGTCTGCGGC22.5974025974025974No Hit
TCTGCCGTGTGACTGGTGGGATGAAGGTAAAGGCAGACCGAGATGAATCC22.5974025974025974No Hit
TCTCCGGCTAAGGTAACGAAGAGGTCACTCTGGTGTAGCTGCTCGACGGG22.5974025974025974No Hit
GCGTAATTCTTGAGCGTACTCATCTGTGAGGCCGGTGGACCGCGCAGAGA22.5974025974025974No Hit
ACCTAGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGCGTG22.5974025974025974No Hit
GTAGGAAGGAGGCCCAGGCCCCATCCGCACAGCAAGTCCCGGTGAGGGGC22.5974025974025974No Hit
AAGAAAATGTGTATTGGCCTAGCTTTCAGCCGGTGTTGTTGTGCATTTAC22.5974025974025974No Hit
GAATTGTAATCCGTTCCTTCTGTAGCTTCCGCACTCGGATCTTTAGCTCC22.5974025974025974No Hit
GGCCCTGGGTGACGTCGTCTTTGTCGACGTCCCCGAGGTCGGCAGCGATG11.2987012987012987No Hit
ATTTCCTCTTTGGGCATGCAGATTAGTAGGAAAAAAAACAGTCTCAAATT11.2987012987012987No Hit
AGCTCGGGGTCTGCTTGGGCCTGAGCCACCCACAGGTCCTTTTCTTCCCC11.2987012987012987No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
AAGGCCATCGCCACTGGCGATTTTTTTGGCACTCTCGATCTGTCTCTTAT11.2987012987012987No Hit
CACTACCGCTGTGATATTTATAATTATTTCCTGATGCTCTGTCTTCCCTT11.2987012987012987No Hit
ACTCAAACCCCATCGCATTACGCTGACGATCGTCGTCATCATGGCCACAA11.2987012987012987No Hit
GTAAATTCCTCATCATGGCGCACAAAGACTCTTGGTGATCTGGACCCACC11.2987012987012987No Hit
GTTTTGATACATGCCACAACATGGATGAACCCTGAGAACACTATGTTCAG11.2987012987012987No Hit
TTACACCAATGACCCATCTGATGAAGAAAAAGATCAGGGACAGAGAGGGC11.2987012987012987No Hit
CCATGCACCGTCCGTCTGTAATCCCATTCCCGGTTCCGGCAGCGAAGATG11.2987012987012987No Hit
GTTCTCGTGTTGATCGACCTCTTGCAGCGATACCTGGTTCGTCGTGGCTA11.2987012987012987No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAGTTGGAACATCTCGTATG11.2987012987012987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCACAGG11.2987012987012987No Hit
ACCTATGGCACGCCGGACCGTCCCATCCACGTGGCAGAGATGATTTCGAA11.2987012987012987No Hit
TCCCAGGCCAGTGAGCTTTACTTGCCGTGTAAAAGGAGGAAAGGGGTGGA11.2987012987012987No Hit
GTATCGCGTCAGGCAGTTGCGTTGGCGCGACGCAATGTGCCGGGCGGCCG11.2987012987012987No Hit
CCAGCAACCCGACGACCATCCCAAGCATGATGTTGATGATCTTGTCCATC11.2987012987012987No Hit
AGTTTACGGAGAAGCTTTTCCGAGTCGCCAGGTTTCACAGGTTCGTTTTT11.2987012987012987No Hit
TTCGTATATTGTGAATTTATGTAAACAGTCAAGCCTTTTTGCTAAGAATA11.2987012987012987No Hit
CTTCCATACTGGCCTGTTGTTCCTTGGCCTCTTTTAATTTGGTCAACAGA11.2987012987012987No Hit
CTTCCTTGTCGCCGTCGCCCTCGGCACCGCCGGTCAGGTTCCACAGCAGC11.2987012987012987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACAAAATG11.2987012987012987No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA11.2987012987012987No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA11.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCAAGTACACTGGTACTTTGCACCGACCAATTATGTTTGCGCAAACGCC11.2987012987012987No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2987012987012987No Hit
GTGCAGGCGCGCTGGCGCAGTACATCGGGCGGCAGGATCTCGCCGTCGGC11.2987012987012987No Hit
GCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGATTA11.2987012987012987Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTAAAGACACTGCTCCCAGTACCAAGTTTTGAAGATGTTTCCATTCCT11.2987012987012987No Hit
TTCCAGGGCAGCGTCTCTCTCAATGTGCTTGTGGTACTCATCCATGGTGG11.2987012987012987No Hit
GGCGAGTACGCCGAGCGGCAGATGTTCACCGAGGTCGCCGCCGGTGGCCA11.2987012987012987No Hit
AGGCTTGCATTCGCTCCCTCCTCCTGGAAGGTCCTTTCCGAAGAGTCCGA11.2987012987012987No Hit
ATTCCAACCAAAACACACAGAATACTGTTCATGAGAGGGTACAACTTGAG11.2987012987012987No Hit
GTCAACCCCATCACAGGCAAGAATCTCAATGCCGAAGACGACGACTGATC11.2987012987012987No Hit
CTGGTACGCCCGCAGACGTGATGAGCCACGGGCTGGCCGCGGCTACCACC11.2987012987012987No Hit
CCTGCTGGGCGATGAAGAGGAAGAAGGTCTCGACAAACTCTGGCTTGCCC11.2987012987012987No Hit
ACATCGATAAGCGCAGGGCCGTTATACGCCAAGGCCTTCTTGATCTGCTT11.2987012987012987No Hit
GTGCCACACTGACCAGCGAAGAGAGAGAGCTGTAGGGCCAGCTGCCGGGC11.2987012987012987No Hit
ACCTAGGCTTAGCCTCACATTTTTACACATGGTATCAGTAAAAAATCTAC11.2987012987012987No Hit
ATAGTGATTAACGTCGTTGTAGTAGGCTTGTGCATTCTCCTGTGGCCACT11.2987012987012987No Hit
GTCTGGTAGAGAGCAGCTGGGGCATCTGCAGAGGGCTCATTTAAGATGAC11.2987012987012987No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC11.2987012987012987No Hit
GGGACACGAGGGCCTCACGCGCGCCACGTCCACCGCCGTGCTGTCGGCGA11.2987012987012987No Hit
GTCGGCATCCATACCATCAAGGGTCAGCGCGCCAATCAGCGTCTGTCCGG11.2987012987012987No Hit
ATGACAGAATGATGTGCTGTGGTGAAACCATGACCTGTCTCTTATACACA11.2987012987012987No Hit
CTCCTGAACCGAGGTGGCGCGCTTGGCCGTGAACACGAGGTCCACCGCCG11.2987012987012987No Hit
GACTGACACCGAGCGCATGGAGGCTGTCCTCGAGGATCCCTATATCCTTA11.2987012987012987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)