FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGGACTAGGACTCCTTGCTTGACCCCATCTCTGGTTCCTGCCCTGGC22.5974025974025974No Hit
AAGGAGAACTGGCGGTCGACATCGCAGCTAAACCACACCGGTTCGCCGGC22.5974025974025974No Hit
TCACAAAGTAGGGGTGGGGGTGGGGTGCAACTACTAGCATCTAGTGGGTA22.5974025974025974No Hit
TCGATGAGCCGCTTGAAGCGCAGGTGGGTGTCCATCGGGGGCGGTTCGAT22.5974025974025974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
GAGGGCCGACTGGGCCCCAGGTCCAGGGGTCTTGGTCCTATTTCCTCCTG22.5974025974025974No Hit
TTGCTGGGGCATAGCGCGACGTGCCGCGGGCGGGTAGGCGAGTGGCATGG22.5974025974025974No Hit
GGGGATGCTTTGCCCCCACCCACCCTGCAGCCTTCTCCACTCTTCCCTCC22.5974025974025974No Hit
AGGTAGTCCCGTGCTGCCCGGCTGCGAGGATCCCAGATACGTCCGATCCG22.5974025974025974No Hit
GGCGCGGACGACAACGCCTGTCGAGTCGATGCGGGAGGCAAGGATGCGAG22.5974025974025974No Hit
AGAAAAAGGAATAATCTATAGAGTAGACAGTAGGTCTGGGGTCTTTGCCT22.5974025974025974No Hit
GTCTTGGTTTGTTGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACT22.5974025974025974No Hit
CTCCAGGCCTAGGTAAATGCACAACAACACCGGCTGAAAGCTAGGCCAAT22.5974025974025974No Hit
ACACCGGCCAGCAGGTGACGCTGGCCTGGGCGCTGCCGTTCAAGCTTGGC22.5974025974025974No Hit
GAAGAGTTCGGCTTCGCCACGGCGCCCCTCGTCGTGAGCGGCTGCGTGAT11.2987012987012987No Hit
GTACGGGCCGTGAAGACTTTAATATCGACTGGCTGGATGATCAACTTGCT11.2987012987012987No Hit
CCCAGAAGGAGGCCAAGAAGGACGAGCCCAAGAGCGGCGAGGAGGCGCTC11.2987012987012987No Hit
GATATCGACTCCGTCGGTATCACCAACCAGCGCGAAACCACCGTGGTGTG11.2987012987012987No Hit
GTGGAGAATAATGCAATCTTGTGTACATGTAGATTTTTTACTGATACCAT11.2987012987012987No Hit
GTCATTGCCTATCATGGGTGCGACGCATCTGTACGGGACAAGGTCATACA11.2987012987012987No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATTGGTTGGTTCAAGTTGAGAGAAGATGGTCAGGGAAGGTTCTTTAAA11.2987012987012987No Hit
GTCATGGTTTCACCACAGCACATCATTCTGTCATCTGTCTCTTATACACA11.2987012987012987No Hit
TGTCTCGGCTGTCATGCCCCGGGCTGCGGGAATGTGCTTGCCTATCTGTT11.2987012987012987No Hit
GTGTGGAATTACAGCAGTAATCAAGCTAAACTTCCTGCCCTCATGTAGTT11.2987012987012987No Hit
CCTTGGCGGCGCCCCTCGACGCCGGGGGCCACCGACACCCCGCGCCCCAG11.2987012987012987No Hit
GTGAACTTCAGTTAAAATTGGATGAACTTCAAGCTTCTGATGTTTCTGTT11.2987012987012987No Hit
TCGGCGTCCTGCAAGTGCTGAAGTTATTGACTCGGTAATGGAGGTGTCAC11.2987012987012987No Hit
CATATTTGCTTTGTGTCTGCCTGCTAATATCTCTGAACATAGTGTTCTCA11.2987012987012987No Hit
GGCGATGTCATTGAGCATAGCCTTGCGACGATCGCCAAAGCCTGGAGCCT11.2987012987012987No Hit
AAGCTACGGTAAACATCTCGCAGAATATCTTGGGGGGAATTTAATAATCT11.2987012987012987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2987012987012987No Hit
GGCTGACAACCTGTACATTGACGTCGCCGCCGGGGTGACCTGTTTTCTGC11.2987012987012987No Hit
TCTGTGGATGGCCCTCAGGGTCCTGGGAGACCCTCTGGGCTGAGGAGGAG11.2987012987012987No Hit
CCCTGGCACTGGGAATGACATCGTCGGGGCGTGAATCCCGAAGTCGATGA11.2987012987012987No Hit
GAGTCAGATAGCGCACGATGGCCTGTCCGACGCTGAGCCTCACAGTCTGC11.2987012987012987No Hit
GATGAGCTTCAGATTTAGCTCAAGTTGTACCCTCTCATGAACAGTATTCT11.2987012987012987No Hit
GGCTGACACCCTGTACCTTGACGTCGCCGCCGGGGTGACCTGTTTTCTGC11.2987012987012987No Hit
GCTCAAGAACCAGCGCAACGATAAGAACTCTGGAAAGGTCGACGAGGACG11.2987012987012987No Hit
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACAT11.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT11.2987012987012987No Hit
GACATAAAGATAACAGCCTAAAAAGTGAAATTTGAGACTGTTTTTTTTCC11.2987012987012987No Hit
CCATAGAGCGGTTGATTGTCAGGCGCATCATTTCAAAGTGGCCCCAATGC11.2987012987012987No Hit
GTCGGCGGACGTGTCTGCGGTGAGGTCACTGCCCCCTCCTCTCGGGACGT11.2987012987012987No Hit
GTATCGCAGGTCGTCTTCGACGGATCCAACGTGGCGGCTCGAGAAAGGGT11.2987012987012987No Hit
GACCGGTACCACGACGAGCGCGGAGAGGTACAGCACCACCACGAGCCGCC11.2987012987012987No Hit
GTCAAGATGTTCGTGTTCAATAACTCCTCGCTGGGCATGGTCAAGCTGGA11.2987012987012987No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2987012987012987No Hit
GAAGTAAATAGCTGCCAGCGCTTGCTGGTAGCGGGCTGGAGGCCAATTTC11.2987012987012987No Hit
ACTGTATGTTCTATGTAGGGCCTGATACAGATGTTACTTGATGTTATTTA11.2987012987012987No Hit
TATATACACACCTGTATGTGTATTGTACATGTGTGTGCCAGTCAAAACCC11.2987012987012987No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTTGGTCGGCGCTTTGCCCTGGAAGGACACCCGCGGCTTGAGGCGAT11.2987012987012987No Hit
GGACTCATCGTCACCCAAGGCATCCTCACCAAGCTGGCGCCTCATGCCCA11.2987012987012987No Hit
TTCCAATATATATCAGCCCTGGGCACTCTCGCCCTGCTGCTCACAGCATG11.2987012987012987No Hit
GCTCGGGCTTCGGGCGCGCACCCACCAAGCCTCCCCCCACCTTCCCCCCA11.2987012987012987No Hit
GGCGGGAGTAGCAGCAATGGCAGCGAAGGGGAGACGGTGTGGAAGAGCAC11.2987012987012987No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA11.2987012987012987No Hit
CCCGTTGACGTCGAACAAGTGCAGATCGCGAGCCAAGGCGGCCGGGTCAC11.2987012987012987No Hit
CCTCTGCACCATCACGCGGGCGGGGCGGTACTCCGTCTACGTCCCTTGTT11.2987012987012987No Hit
TTATCAAGGTGTTTGTTCCGGAAGGCTACGACGAGAGCATTTTCCTTAGT11.2987012987012987No Hit
ATCGAGAGTGCCAAAAAAATCGCCAGTGGCGATGGCCTTCTGTCTCTTAT11.2987012987012987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)